简介：不要悲哀,不要糊里糊涂地迷失了自我;世界上没有绝对的事,只要努力,一切都会好起来的。

简介：作文要求：关键词：感悟。处处留心皆学问，极平常的一件小事，或者一个现象，只要我们认真的思考，就会有所发现。请你就生活之中的某一点，说说你的感悟吧。

简介：这是一款做工十分精美的解谜类游戏,但是情节与大多数同类游戏不同。以往有关于机器人救援的游戏大都是某地发生灾难,人类的搜救人员无法进入这些危险的地方,所以派出了钢筋铁骨的救援机器人拯救人类安危。但是这款游戏则反其道而行,救援的对象变成了机器人,而玩家需要做的就是利用机智的头脑,控制机器人顺利逃离苦难,拯救机器人于水火之中。

简介：一天，我在课室里看书，正看得入迷，突然，我的好朋友小三跑过来张口就问了我一个问题：“1＋1等于几？”我轻轻地叹了一口气，平静地回答：“等于2。”

简介：在Saccharomycescerevisiae，必要基因CDC13编码telomeric遗传上并且身体上与Stn1p和Ten1p交往的搁浅单人赛的DNA有约束力的蛋白质，并且为telomere结束保护和telomere长度控制被要求。Ten1由参予telomere长度规定和染色体结束保护的分子的机制留下逃犯。在这个工作，我们用净化的recombinantCdc13p和Ten1p在胶化过滤分析观察了Cdc13p和Ten1p的一个弱相互作用。Ten1p本身展出一项弱DNA有约束力的活动，但是提高telomericTG1鈥吗？Cdc13p的DNA有约束力的能力。Cdc13p是有Ten1p的co-immunoprecipitated。在变异的ten1-55或ten1-66房间，在Ten1p和Cdc13p之间的损害相互作用与telomericDNA导致长得多的telomeres，以及Cdc13p的一个减少的协会。一致地，Ten1-55和Ten1-66异种蛋白质没能刺激telomeric在vitro的Cdc13p的DNA有约束力的活动。这些结果建议Ten1p提高telomericCdc13p到的DNA有约束力的活动否定地调整telomere长度。

简介：摘要目的在体外转录快速获得原核生物来源核酶P的核心催化亚基M1RNA。方法以大肠埃希菌DH5α菌液为模板，应用聚合酶链式反应的方法扩增M1RNA基因，并将该基因置于T7启动子下游，其聚合酶链式反应产物经电泳及测序鉴定。以M1RNA基因为模板，以32P标记，在T7 RNA聚合酶的作用下体外转录M1RNA。将产物以尿素变性聚丙烯酰胺凝胶分离并观察结果。结果应用聚合酶链式反应的方法从大肠埃希菌基因组扩增M1RNA基因，经凝胶电泳观察及测序鉴定，证实成功在体外扩增M1RNA基因，并置于T7启动子下游。在T7 RNA聚合酶的催化下，应用尿素变性聚丙烯酰胺凝胶电泳分离产物，结果显示377nt处可见与预期相符的条带，证实成功在体外获得M1RNA。结论成功在体外快速获得具有独立催化功能的M1RNA，基于此的基因沉默技术具有发展成新型反义核酸药物的良好前景。

简介：Hebert报道55例 ALL中22例有MTS1/P16基因缺失,43例ANLL中有 6例MTS1/P16 Exon2纯合缺失,43例ANLL MTS1/P16纯合缺失率为14.0%(6/43)

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简介：摘要目的对1例超声影像提示左足足内翻的胎儿进行遗传学分析，探讨其染色体拷贝数变异与临床表型的相关性。方法选取2020年10月14日于台州医院就诊的1例5p缺失综合征胎儿为研究对象。采集胎儿的羊水及其父母的外周血样，进行G显带核型分析，应用拷贝数变异测序(CNV-seq)检测胎儿染色体的微缺失与微重复，进一步用荧光原位杂交(FISH)技术进行家系验证。结果胎儿及其父母的G显带核型分析均未见明显异常，CNV-seq发现胎儿5号染色体存在23.12 Mb的拷贝数缺失，7号染色体存在21.46 Mb的拷贝数重复，FISH进一步验证胎儿母亲为隐匿性t(5；7)(p14.3；q33)携带者，胎儿的拷贝数变异遗传自母亲。结论CNV-seq联合FISH技术能有效诊断出隐匿性5p缺失综合征，避免患儿的出生，为产前遗传咨询提供理论依据。

简介：摘要目的分析1例p表型个体的血清学特征和分子机制。方法选取2021年5月在嘉兴市中心血站进行血型鉴定的1例p表型个体为研究对象。用血型血清学方法鉴定其ABO、RhD、P1PK血型和意外抗体。采用PCR-直接测序法(PCR-SBT)对编码P1和PK抗原的α1，4-半乳糖基转移酶基因(A4GALT)的编码区进行测序分析。结果该个体的血型为A型、RhD阳性、P1PK系统为罕见的p表型，血清中存在抗-PP1Pk。测序结果显示其A4GALT基因编码区存在c.343A>T纯合变异。结论A4GALT基因c.343A>T纯合变异很可能导致了p表型个体。

简介：AbstractObjective:The objective of this study was to investigate the expression levels of microRNA-141-5p(miRNA-141-5p), MAPK1 and neutrophil elastase in patients with and without preeclampsia (PE), and the relationship between miRNA-141-5p and MAPK1 with respect to the secretion of elastase by neutrophils in patients with PE.Methods:Thirty patients with PE and 30 healthy pregnant (HP) women were recruited from The Second Hospital of Shanxi Medical University, Taiyuan, China, between February 2017 and July 2018. Neutrophils were isolated from 8 mL peripheral blood samples and cultured. We recorded neutrophil count and morphology during culture. Apoptosis was detected by flow cytometry in different groups at 0, 24, and 48 h. The expression levels of elastase were detected in neutrophils by enzyme-linked immunosorbent assay, whereas the expression levels of miRNA-141-5p in peripheral blood neutrophils were detected by real-time polymerase chain reaction. We used TargetScanHuman Release 7.2 to analyze the target genes of miRNA-141-5p. The expression of MAPK1 in peripheral blood neutrophils was detected by western blotting. Data were analyzed by SPSS version 21.0 software, and comparisons between groups were carried out with the Student t test.Results:There was no significant difference between the PE and HP groups (P > 0.050) with regard to age or body mass index. The weight of newborns in the PE group (2846.00 ± 600.00 g) was significantly lower than that in the HP group (3055.00 ± 230.68 g). The number of neutrophilic granulocytes(NGs) in blood samples from the PE group was significantly higher than that in the HP group (P = 0.003). There was no significant difference between the groups with regard to morphology. Apoptosis in the PE group was delayed when compared with the HP group at different time points. The P value of apoptosis in the PE and HP groups were respectively 0.790, < 0.001 and 0.030 at 0 h, 24 h and 48 h. The expression levels of miRNA-141-5p in the PE group were significantly lower than those in the HP group (P < 0.050). The expression levels of MAPK1 in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050) by western blot. The expression levels of elastase in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050). Furthermore, the number of NGs in peripheral blood from the PE group was higher than that of the HP group; however, the levels of apoptosis were lower. The expression levels of miRNA-141-5p in NGs decreased, the expression of MAPK1 increased, and the secretion of neutrophil elastase in the NG medium increased in the PE group than those in the HP group.Conclusion:Collectively, our analysis suggested that miRNA-141-5p may be involved in the pathogenesis of PE by regulating the MAPK1 signaling pathway to activate neutrophils and increase the secretion of elastase.

简介：HIV-1p24察觉提供一个工具帮助HIV-1感染的早诊断，追踪疾病的前进并且估计antiretroviral治疗的功效。在对HIV-1的现在的学习，三monoclonal抗体(mAbs)p3JB9，p5F1和p6F4，p24被产生。所有mAbs能检测HIV-1B，HIV-1Ada-M，HIV-174vmAbsp5F1和p6F4的p24能检测HIV-1KM018，当p3JB9不能时。三mAbs没与HIV-2ROD，HIV-2CBL-20和SIVagmTyo-1反应。p5F1的公认的epitope位于Gag氨基酸区域DCKTILKALGPAATLEEMMTAC。p5F1被用来与兔子anti-p24浆液建立修改三明治ELISA并且显示出好特性和高敏感，它被用来在研究测量HIV-1p24抗原层次。

简介：目的：探究新辅助化疗对宫颈鳞癌中Bub1、p53的影响及其机理。方法：在我院接受治疗的宫颈鳞癌患者40例均采用新辅助化疗治疗。根据组织摄取部位不同分为癌旁组和癌中组。观察治疗前后癌旁组和癌中组的Bub1、p53的含量。结果：癌中组的Bub1均增高,p53均降低,接近癌旁组。结论：新辅助化疗治疗宫颈鳞癌临床疗效显著,其机理可能是通过升高Bub1、降低p53含量进行调节,对肿瘤细胞的生长起到了抑制的作用。

简介：WereportedinthismanuscriptthatTGF-β1inducesapoptosisinAML12murinehepatocytes,whichisassociatedwiththeactivationofp38MAPKsignalingpathway.SB202190,aspecificinhibitorofp38MAPK,stronglyinhibitedtheTGF-β1-inducedapoptosisandPAI-1promoteractivity.TreatmentofcellswithTGF-β1activatesp38.Furthermore,over-expressionofdominantnegativemutantp38alsoreducedtheTGF-β1-inducedapoptosis.Thedataindicatethattheactivationofp38isinvolvedinTGF-β1-mediatedgeneexpressionandapoptosis.

简介：1．a^1p=p√a(p≠0，a>0)例1、为多少?分析已知x^1/2+x^1/2=8，则x+1/x的值为多少？分析指数为分数，考虑用a^1p=p√a。

简介：Thespectrumofisospin3/2Δ++(1232)excitedstatesisoneofthemostimportantissuesinhadronicphysicsandisattractingmuchattentionbecauseitisthemostexperimentallyaccessiblesystemcomposedofthreeidenticalvalencequarks.However,ourknowledgeontheseresonancesmainlycomesfromoldNexperimentsandisstillverypoor[1].Intheenergyregionaroundorabove2.0GeV,therearestillmanytheoreticalpredictionsofmissingΔstateswhichhavesofarnotbeenobserved.Searchingforthesemissingstatesfromotherproductionprocessesisnecessary.ApossiblenewexcellentsourceforstudyingtheseΔresonancecomprisesthe+p!K+Σ+(1385)andpp!nK+Σ+(1385)reactions,whichhaveaspecialadvantage,i:e:,thereisnocontributionsfromisospin1/2nucleonresonancesduetotheisospinandchargeconservations.

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简介：Objective:ToinvestigatetheeffectofsubstanceP(SP)ongeneexpressionoftransforminggrowthfactorβ-1(TGFβ-1),transforminggrowthfactorreceptor-1(TGFR-1)andtransforminggrowthfactorreceptor-2(TGFR-2)infibroblastsculturedinvitrofromrat'sgranulationtissues.Methods:Thefibroblastsfromthegranulationtissuesintheskeletalmuscleofrat'shindlimbsinjuredbyformaldehydewereculturedinvitro.Whendifferentconcentrations(10-9-10-5mol/L)ofSPwereaddedintotheculturemedium,thechangesofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2intheculturedfibroblastswereobservedwithreversetranscriptionpolymerasechainreactionatdifferentintervals(0,3,6,12and24hoursafterincubation).Results:ThegeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblastsculturedfromrat'sgranulationtissueswasup-regulatedbySP.ThepeaklevelofthemRNAexpressionwasfoundat10-8mol/LSPandtheup-regulationeffectwasnotfoundat10-5mol/Land10-6mol/L.ThepeaklevelsofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblaststreatedwithSPwereachievedat6and12hours,respectively.Conclusions:SPhasup-regulationeffectonthegeneexpressionofTGFβ-1,TGFR-1andTGFR-2infibroblastsfromrat'sgranulationtissuesinvitro,andtheeffectisrelatedtodifferentstimulatingconcentrationsofSP.Itmaybeconcernedwithproliferationanddifferentiationoffibroblastsandformationofscartissuesduringwoundhealing.

简介：为什么企业少收或不收目标顾客的钱．甚至倒找钱给顾客还能赚钱7是当今时代网状经济的特质造就了企业零价格销售照样赢利的奇迹!北京大学光华管理学院王建国教授独创的1P理论突破传统4P营销思维，提出了与当今网状经济时代匹配的崭新商业模式，为企业的经营管理者带来了新的思路．出路与财路。

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