简介:Undisturbedsoilcorewithmanymacroporesanddisturbedsoilcorewithonlyonemacropore(diameteris10mm)wereprobedbyx-raycomputedtomography(CT).Thesize,number,shapeandcontinuityofmacroporesinthetransverseandverticalsectionsofsoilwerecharacterizedusingCTscanningimages.TheprobabilitydensitiesofmacroporesinthetransversesectionofsoilcoreexhibitedalogarithmicΓdistribution.ResultsindicatedthatCTscanningwasapromisingnondestructivemethodforcharacterizingmacroporesinsoils.
简介:InordertoexplorethefeasibilityofusingX-raytomeasuremoisturecontentinthedryingprocessandhaveafurtherstudyontheeffectofprecisionofmoisturecontentwhenscanningthroughdifferentgraindirections,X-rayscanningmethodandweightingmethodwereusedtomeasureaveragemoisturecontentofCunninghamialanceolataduringhotairdrying.Theresultsshowthatthemoisturecontentvaluesoftwomethodswereveryclosetooneanother,thedeterminationcoefficientsofbothwerehighlycorrelatedwithavalueover0.99,andtheabsolutedeviationwasbelow2%;whenscanningalongradialdirectionandlongitudinaldirection,theprecisionsofaveragemoisturecontentwerehigherthanthatofthetangentialdirection,butthedeviationcanbenegligibleintheactualmeasurement;inthepracticalapplication,theeffectofgraindirectiononprecisionofmoisturecontentmeasurementcouldnotbetakenintoaccountwhenusingX-raytomeasurethemoisturedistributionofwood.
简介:油菜素类固醇参与了植物节间的发育过程,类固醇5α-还原酶基因(GhDET2)是调控该物质生物合成的一个关键基因。为了明确棉花不同株型种质GhDET2在基因组中的异同,本研究依据GenBank中该基因的mRNA序列设计引物,对11份适宜机采紧凑型和1份松散的棉花材料基因组DNA扩增及PCR产物测序,采用GeneiousPro软件对编码区序列进行分析比较。结果显示DET2基因在参试材料间一致性为98.9%,在编码区发现18个碱基易突变位点,其中涉及到编码氨基酸变化的碱基位点6个,其中松散型材料特异位点1个。依据氨基酸序列相似度的聚类结果与果节长度数据符合程度很好,可以推测GhDET2基因碱基序列变化引起的氨基酸的改变,可能影响油菜素类固醇的合成代谢,进而调节棉花果枝上果节的伸长。
简介:Genotypingbysequencing(GBS)istherecentapproachofnext-generationsequencingtechniquefordiscoveringandgenotypingsinglenucleotidepolymorphisms(SNPs)incropspecies.Genotypicvariationstudies(SNPsandinsertion-deletions/InDels)wereperformedusingfourricelinesbasedonGBSdatabyaligningtothereferencegenomeNipponbare.LocalaromaticricelandraceTulaipanjiwascrossedwithRanjit,andtwodistinctlineswereidentifiedfromtheprogenies:onelinewithawnsandaromatraitsandtheotherwithoutawnsandaroma.TotalnumberofSNPsandInDelsidentifiedwere52810and4327atreaddepth10,respectively.OutofthetotalpolymorphicSNPs/InDels,16490wereintergeneric,7812wereinsidegene,and4435wereintronic.Phylogenetically,Tulaipanjiwasclosertothereferencegenomenipponbare.Basedonrecurrentparentgenomeanalysis,outof10013alleles,92.52%wasintrogressedintoprogeny-awnfromTulaipanjiand7.48%fromRanjit,whereasprogeny-awnlesscarried89.19%allelesfromRanjitandonly10.81%allelesfromTulaipanji.Inaddition,progeny-awnwasthehighestheterozygous(83.88%)andprogeny-awnlesswastheleast(2.24%)atthisfifthgenerationofrecombinantinbredlines.TheseSNPvariationsmaybelinkedtothephenotypictraitsandcanbeutilizedincropimprovementthroughlinkagemapping.TheseresultssuggestthataddingahighdensityofSNPmarkerstoamappingorbreedingpopulationthroughGBShasagreatvaluefornumerousapplicationsinricebreedingandgeneticsresearch.