简介:CD3-specificmonoclonalantibodywasthefirstoneusedforclinicalpracticeinfieldoftransplantation.Recently,renewedinterestshaveelicitedinitscapacitytopreventautoimmunediabetesbyinducingimmunetolerance.Inthisstudy,wetestedwhetherthisantibodycanalsobeusedtotreatanotherkindofautoimmunediseasemyastheniagravis(MG)andexploredthepossiblemechanisms.MGiscausedbyanautoimmunedamagemediatedbyantibody-andcomplement-mediateddestructionofAChRattheneuromuscularjunction.WefoundthatadministrationofCD3-specificantibody(Fab)2toananimalmodelwithexperimentalautoimmunemyastheniagravis(EAMG)(B6micereceived3timesofAChR/CFAimmunization)couldnotsignificantlyimprovetheclinicalsignsandclinicalscore.Whenthepossiblemechanismsweretested,wefoundthatCD3antibodytreatmentslightlydown-regulatedtheT-cellresponsetoAChR,modestlyup-regulationthemusclestrength.AndnosignificantdifferenceinthetitersofIgG2bwasfoundbetweenCD3antibodytreatedandcontrolgroups.ThesedataindicatedthatCD3-specificantibodywasnotsuitablefortreatingMG,anantibody-andcomplementmediatedautoimmunedisease,afterthisdiseasehasbeenestablished.TheroleofCD3-specificantibodyintreatingthiskindofdiseaseremainstobedetermined.
简介:摘要目的探讨真菌细胞壁(1-3)-β-D葡聚糖体外检测的影响因素。方法实验组将白色假丝酵母菌分别用正常血浆、无热原蒸馏水、0.9%的生理盐水、0.02%两性霉素B生理盐水和0.75%氟康唑生理盐水稀释为0.05麦氏单位(含白色假丝酵母菌1×107CFU/ml),对照组将上述各液稀释100倍,采用免疫沉淀试验(G试验)检测其10次(1-3)-β-D葡聚糖含量,并进行体外培养,以此探讨生理盐水、抗真菌药物对(1-3)-β-D葡聚糖体外检测的影响。结果实验组中,正常血浆葡聚糖含量为(92.5±14.2)ug/L,蒸馏水组含量为(94.5±15.2)ug/L,生理盐水组含量为为(93.6±14.8)ug/L,两性霉素B组含量为(95.5±16.2)ug/L,氟康唑组(97.3±16.8)ug/L,经统计检验组内结果间无显著性差异(P>0.05)。对照组中正常血浆含量为(6.8土2.7)ug/L,蒸馏水组含量为(6.1±2.4)ug/L,生理盐水组含量为为(7.3±2.0)ug/L,两性霉素B含量为(6.8±2.5)ug/L,氟康唑组(6.7±2.8)ug/L,经统计检验组内间结果无显著性差异(P>0.05)。实验组与对照组比较有显著差异(P<0.05)。结论体外真菌细胞壁(1-3)-β-D葡聚糖含量变化与液体介质无关,与真菌治疗药物无关,与真菌数量密切相关。
简介:摘要目的分析高危儿2-3岁步行周期中踝关节异常姿势情况。方法运用直接目测观察法结合间接录像观察法对我院高危儿门诊随访的160例2-3岁小儿进行步态分析,重点观察踝关节在步行周期中的运动姿势。并根据一岁前是否曾因运动落后或姿势异常进行过早期干预分为干预组(90例),未进行过康复训练的患儿为空白组(70例),对两组患儿踝关节异常姿势发生率进行比较。结果①160例小儿在步行周期中出现踝关节姿势异常的总发生率占15%,干预组姿势异常发生率(10.0%)较空白组(21.4%)显著降低,两者差异具有统计学意义(P<0.05);②其中首次着地前脚掌着地占3.7%,站立相足外翻占5.6%,站立相足内翻占1.3%,迈步初期足趾拖地占3.1%,迈步相过度跖屈占1.3%;③干预组高危儿踝关节姿势异常最主要表现为“首次着地前脚掌着地”,空白组高危儿踝关节姿势异常最主要表现为“站立相足外翻”。结论高危儿在2-3岁独走步行周期中踝关节姿势异常发生率较高,早期干预能有效的降低踝关节姿势异常的发生率,因此在高危儿随访门诊中应加强小儿步态的随访监测,对异常姿势给予及时康复干预。