简介：Theexpressionofglucoseregulatedprotein94(GPR94)duringthetreatmentofhumancolorectalcarcinomacelllineCloneAcellswithcodiumbutyratewasstudied.Dodiumbutyrate(SB)cancausefunctionalandmorphologicaleffectsonCloneAcellsincludinggrowtharrestatG0/G1stageandcelldifferentiationasobservedbymorphologicalchanges,MTTandflowcytometryassays,aswellasreducedGrp94geneexpressionasshownbyNorthernblotandWesternblotassays.ThepossiblemechanismofthecorrelationbetweenGrp94geneexpressionandtumorgrowthinhibitionandcelldifferentiationisbrieflydiscussed.
简介：Thenorepinephrinetransporter(NET)isamemberoftheNa^+/Cl^-dependentneurotransmittertransporterfamilyandconstitutesthetargetofseveralclinicallyimportantantidepressants.TodelineatethecriticalaminoacidresiduesandthefunctionofC-terminalinregulatingtransportactivityofNET,hereweconstructedtwositemutants(V70F,F72V;V70I,F72V)andoneC-terminaltruncatedmutant(Δ611-617).ThewildtypeandmutantsofNETwereexpressedinXenopusoocytesbyinjectionoftheircRNA.Wefoundthatallofthesemutantslosttheirtransportactivity.TheseresultsindicatethattheaminoacidresiduesofV70andF72,andthelastsevenaminoacidsofC-terminalareessentialtothetransportactivityofNET.
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