简介:摘要近年来免疫检查点抑制剂是癌症免疫治疗研究的一个重要里程碑。靶向程序性死亡受体1(PD-1)、程序性死亡受体-配体1(PD-L1)或细胞毒性T淋巴细胞相关抗原4(CTLA-4)的单克隆抗体在临床试验和临床应用中发挥着巨大的作用,并且当治疗有效时可产生持久的临床反应,已经成功获批应用于多种肿瘤的治疗。但是,因为其总体的应答率不高,仅使部分患者受益。因此,亟需探索新型的免疫检查点抑制剂,并且探究其作用机制进一步发展肿瘤免疫疗法。CD112R是免疫细胞表达的免疫球蛋白超家族受体,与肿瘤细胞表达的 Nectin-2/Necl 家族配体(CD112)相互结合,在肿瘤免疫治疗中具有很大的潜力,是新一代的抑制性免疫检查点。本文将对CD112-CD112R轴的分子结构与在肿瘤免疫反应中的作用进行阐述,探讨在癌症免疫治疗中的潜在应用。
简介:AbstractBackground:The mortality rate among patients with nasopharyngeal carcinoma (NPC) has improved significantly with the advent of chemoradiotherapy strategies. However, distant metastasis remains problematic. Tumor-specific reactivity in cancer patients has been detected exclusively in CD39+ T cells, particularly in CD39+CD103+ T cells. Circulating cancer-specific T cells are important for protecting against metastasis. This study aimed to evaluate the predictive value of circulating CD39+CD8+ T cells for metastasis in patients with NPC.Methods:We performed a cross-sectional, longitudinal study of 55 patients with newly diagnosed NPC of stage III-IVa. All patients were initially treated with standard combined chemoradiotherapy. Blood samples were obtained from 24 patients before and at 1 month and 6 months after treatment. T cell expression of CD39 and CD103, together with the markers of T cell exhaustion programmed death-1 (PD-1)/T cell immunoglobulin and mucin domain-containing protein 3 (Tim-3) and markers of cell differentiation CD27/CC-chemokine receptor 7/CD45RA, was examined by flow cytometry. The Wilcoxon rank-sum test analysis was used to analyze the differences between two groups. Kaplan-Meier analysis was used for analysis of progression-free survival (PFS).Results:The expression of circulating CD39+CD8+ and CD39+CD103+ CD8+ T cells was significantly higher in patients without distant metastasis (CD39+CD8+: 6.52% [1.24%, 12.58%] vs. 2.41% [0.58%, 5.31%], Z=-2.073, P=0.038 and CD39+CD103+ CD8+: 0.72% [0.26%, 2.05%] vs. 0.26% [0.12%, 0.64%], Z=-2.313, P = 0.021). Most CD39+ T cells did not express PD-1 or Tim-3. Patients with high expression of CD39+CD103+CD8+ T cells had better PFS than patients with low expression (log rank value = 4.854, P = 0.028). CD39+CD8+ T cells were significantly elevated at 1-month post-treatment (10.02% [0.98%, 17.42%] vs. 5.91% [0.61%, 10.23%], Z = -2.943, P = 0.003). The percentage of advanced differentiated CD8+ T cells also increased at 1-month post-treatment compared with pre-treatment (33.10% [21.60%, 43.05%] vs. 21.00% [11.65%, 43.00%], Z =-2.155, P = 0.031). There was a significant correlation between elevated CD39+CD8+ T cells and increased effector memory T cells (intermediate stage: r = 0.469, P = 0.031; advanced stage: r = 0.508, P = 0.019).Conclusions:CD39+CD8+ circulating T cells have preserved effector function, contributing to an improved prognosis and a reduced risk of metastasis among NPC patients. These cells may thus be a useful predictive marker for a better prognosis in patients with NPC.
简介:摘要目的探究CD44.CD24在乳腺癌中的表达情况及意义。方法收集2008年1月1日至2013年1月1日本院本科室收治的160例乳腺癌患者,作为对照组;20例单纯性乳腺小叶增生和20例单纯性乳腺小管增生患者,作为实验组。应用双染免疫组织化学方法检测其CD44和CD24的表达情况。通过对检测结果进行分析,探究CD44和CD24在乳腺癌中的表达情况及意义。结果对本次研究分析后,对照组单纯性乳腺小叶增生和单纯性乳腺小管增生患者,CD44、CD24细胞的阳性率分别为10.00%和15.00%、15.00%和20.00%,经χ2检验,P>0.05,差异无统计学意义;实验组乳腺癌患者CD44、CD24细胞的阳性率均>65.00%,经χ2检验,P<0.05,差异有统计学意义。结论CD44、CD24在乳腺癌中具有比较高的表达率,其较高的表达率与乳腺癌的发生有关。
简介:AIM:ToassesstheutilityofanautologousCD34+andCD133+stemcellsinfusionasapossibletherapeuticmodalityinpatientswithend-stageliverdiseases.METHODS:Onehundredandfortypatientswithendstageliverdiseaseswererandomizedintotwogroups.Group1,comprising90patients,receivedgranulocytecolonystimulatingfactorforfivedaysfollowedbyautologousCD34+andCD133+stemcellinfusionintheportalvein.Group2,comprising50patients,receivedregularlivertreatmentonlyandserveda...
简介:CD4^+CD25^+调节性T细胞是一类具有特殊免疫调节功能的T细胞亚群,它所介导的免疫抑制在移植免疫耐受的诱导和维持中起关键作用。本文对近年来CD4^+CD25^+调节性T细胞的作用机制及免疫耐受等方面的研究进行了综述。
简介:[摘要]目的 探讨CD38及CD138在宫腔粘连患者子宫内膜组织中的表达情况及其临床意义。方法 选取本单位收治的80例宫腔粘连患者作为研究组,另选取同期在本单位行宫腔镜检查的非宫腔粘连患者80例为对照组。免疫组化法检测两组子宫内膜组织中CD38及CD138表达情况,分析CD38及CD138表达与宫腔粘连患者临床病理特征的关系,并采用ROC曲线分析CD38及CD138对宫腔粘连的诊断价值。结果 研究组CD38与CD138阳性率均高于对照组(P<0.05)。CD38与CD138表达与宫腔粘连患者闭经、AFS分级有关(P<0.05)。CD38与CD138联合诊断宫腔粘连的AUC为0.919,敏感度、特异度、准确度分别为88.65%、73.80%、85.50%,均大于二者单独诊断(P<0.05)。结论 宫腔粘连患者子宫内膜组织中CD38与CD138表达上调,与患者临床病理特征密切相关,可作为诊断该疾病预防的生物标志物。
简介:本研究通过检测大肠癌患者外周血CD4+、CD25+、CD127lo/-Treg的水平,Ⅳ期大肠癌患者外周血CD4+、CD25+、CD127lo/-Treg高于Ⅱ期大肠癌患者(P<,方法流式细胞术检测36例大肠癌患者及30例健康人外周血中CD4+、CD25+、CD127lo/-Treg的变化
简介:目的初步探讨CD4Cr细胞表达的腺苷CD39和CD73在过敏性哮喘患者外周血中的水平变化及与叉头蛋白3(FoxP3)、GATA结合蛋白3(GATA3)和维A酸相关孤儿受体(ROR)-γtmRNA相关性。方法:选择54例对屋尘螨过敏的慢性持续期哮喘患者[间歇状态和轻度持续哮喘组(间歇.轻度组)29例,中重度组25例]和45名健康志愿者.通过荧光实时定量PCR方法检测并比较研究对象外周血CD4+T细胞中CD39、CD73、FoxP3、GATA3和ROR-γtmRNA水平。结果:CD39mRNA水平正常对照组明显高于间歇-轻度组(P=0.000),间歇.轻度组明显高于中重度组(P=0.001)。CD73mRNA水平正常对照组明显高于间歇-轻度组(P=0.001),哮喘亚组间差异无统计学意义(P=0.126)。哮喘组CD4+T细胞表达的CD39mRNA与FoxP3mRNA水平呈显著正相关性(r=0.607。P=0.000),与GATA3mRNA水平呈显著负相关性(r=-0.505,P=-0.000),与ROR-γtmRNA无显著相关性(r=-0.287,P=0.054)。哮喘组CD4+T细胞表达的CD73mRNA与FoxP3和ROR-γtmRNA水平无显著相关性(r=0.110,P=0.450;r=-0.227,P=0.138),与GATA3mRNA水平呈显著负相关性(r=-0.286,P=-0.040)。结论:CD4+T细胞表达的CD39和CD73在过敏性哮喘免疫反应过程中起到重要作用。
简介:目的研究乳腺浸润性微乳头状癌(invasivemicropapillarycarcinoma,IMPC)的干细胞表型,从干细胞和上皮间质转化(epithelial—mesenchymaltransition,EMT)角度探讨IMPC高侵袭、高转移恶性生物学行为的原因。方法选取术前未经放化疗治疗患者的IMPC82例和乳腺非特殊型浸润性导管癌(invasiveductalcarcinomanototherwisespecified,IDC—NOS)80例石蜡包埋组织标本切片,通过免疫组织化学双染技术检测两组肿瘤组织中CD44^+/CD24^-/low(CD24^-)和CD24^+的表达、定位和分布情况,并分析其与各临床病理学特征之间的关系。定量资料采用Student’st检验,定性资料采用Mann-WhitneyU检验、Kruskal—Waliis检验、x^2检验或校正x^2检验。两组之间相关性采用Spearman's秩相关分析。结果(1)IMPC组肿瘤细胞的CD44^+/CD24^-/low阳性表达率(48.8%,40/82例),明显高于IDC—NOS组(31.3%,25/80例)(x^2=5.180,P=0.023)。(2)53.7%(44/82例)的IMPC微细间质组织内见单个散在的CD44^+/CD24^-/low肿瘤细胞,且该细胞免疫组织化学染色Vimentin及α—SMA阳性,E—Cadherin阴性。IDC—NOS间质内罕见CD44^+/CD24^-/low肿瘤细胞。(3)IMPC微乳头结构中CD44^+/CD24^-/low与间质内的CD44^+/CD24^-/low阳性表达细胞呈明显正相关(r=0.516,P〈0.001),并且IMPC微乳头结构及间质中CD44^+/CD24^-/low阳性表达在有无淋巴管侵犯和有无淋巴结外软组织浸润方面的差异均有统计学意义(P〈0.050),即有淋巴管侵犯及淋巴结外软组织浸润者CD44^+/CD24^-/low阳性表达率较高。(4)IMPC组中CD24^+细胞阳性表达率79.3%(65/82例),明显高于IDC-NOS组(60.0%,48/80例)(x^2=7.126,P=0.008),且IMPC中淋巴结转移阳性组CD24的表达高于阴性组,差异有统计学意义(x^2=8.834,P=0.003)。结论IMPC肿瘤细胞中干细胞
简介:Usingtwo-colourflowcytometry>200antibodiessubmittedtothe8thInternationalWorkshopofHumanLeukocyteDifferentiationAntigens(HLDA8)havebeenanalyzedfortheirreactivitywithrestingandactivatedCD203c+basophils.Fourantibodieseithernon-reactiveorweaklyreactivewithrestingbasophilsexhibitedanincreasedreactivitywithbasophilsactivatedbyanti-IgE-mediatedcross-linkingofthehighaffinityIgEreceptor(FcεRI).TheseincludeantibodiesagainstCD164(WS-80160,cloneN6B6andWS-80162,clone67D2),aswellastworeagentswithpreviouslyunknownspecificitiesthatwereidentifiedasCD13(WS-80274,cloneA8)andCD107a(WS-80280,cloneE63-880).Theactivationpatternsfollowedeitherthe'CD203c-like'or'CD63-like'activationprofile.TheCD203cprofileischaracterizedbyarapidandsignificantupregulation(ofCD13,CD164,andCD203c),reachingmaximumlevelsafter5-15minofstimulation.ThePhosphoinositide-3-kinase(PI3K)-specificinhibitorWortmannininhibitedtheupregulationofthesemarkerswhereas12-O-tetradecanoyl-phorbol-13-acetate(TPA)inducedarapidandFcεRI-independentupregulationwithin1-2min.IntheCD63profile,maximumupregulation(ofCD63andCD107a)wasdetectedonlyafter20-40min,andupregulationbyTPAreachedmaximumlevelsafter60min.Insummary,ourdataidentifyCD13,CD107a,andCD164asnovelbasophil-activationantigens.Basedontimekineticsofupregulation,wehypothesizethatmoleculesofthe'CD203cgroup'andthe'CD63group'arelinkedtotwodifferentmechanismsofbasophilactivation.
简介:目的检测急性脑梗死患者CD19^+-CD25^+、CD19^+-CD25^-B淋巴细胞、免疫球蛋白和补体C3的含量并探讨其临床意义。方法根据病史及头颅CT或MRI明确疾病诊断。抽取69例急性脑梗死、115例脑出血患者、41例正常对照者静脉血各4mL,采用流式细胞仪检测CD19^+-CD25^+、CD19^+-CD25^-B淋巴细胞百分比,采用散射比浊法检测免疫球蛋白和补体C3含量,并结合不同的病程、不同影像学评分和不同的神经功能评分进行分析比较。结果脑梗死和脑出血急性期CD19^+-CD25^+、CD19^+-CD25^-B淋巴细胞、免疫球蛋白和补体C,的差异无统计学意义(P均〉0.05)。脑梗死急性期CD19^+-CD25^+B淋巴细胞百分比、IgG、补体C3含量均较恢复期及对照组显著增高(P均〈0.05)。脑梗死恢复期各项体液免疫指标与对照组之间差异无统计学意义(P均〉0.05)。不同影像学评分患者之间CD19^+-CD25^+、CD19^+-CD25^-B淋巴细胞百分比差异有统计学意义(P均〈0.05)。脑梗死急性期神经功能评分与各体液免疫指标间无相关(P均〉0.05)。结论脑梗死与脑出血存在着同样的体液免疫功能改变。这种改变可能与应激、病变部位及病变范围有关。脑梗死病灶越大,体液免疫改变越明显;随着应激的消逝,体液免疫功能逐渐恢复。
简介:TheeffectofCdionsonsalmonspermDNAwasstudiedbymeansofcirculardichroism(CD),Ramanspectroscopy,X-rayphotoelectronspectroscopy(XPS)andfluorescencespectroscopy.TheCDspectralandfluorescentprobe-acriflavineresultsindicatethattheDNAunderwentaconformationchangeupontheadditionofCdions.XPSandRamanstudiesrevealthatthereexistedinteractionsbetweenCdionsandthephosphategroupsoftheDNA.Inaddition,anewbandappearedat803cm-1intheRamanspectra,whichcanbeattributedtocharacterizing"marker"bandofA-DNA.ItisconcludedthatCdionscanbecoordinatedbythephosphategroupsoftheDNAandinducetheconformationchangesoftheDNAfromB-DNAtoA-DNA.