简介:Rabbitlimbalcornealepithelialcells,cornealendothelialcellsandkeratocyteswereculturedonamnioticmembrane.Phasecontrastmicroscopeexaminationwasperformeddaily.Histologicalandscanelectronmicroscopicexaminationswerecarriedouttoobservethegrowth,arrangementandadhesionofcultivatedcells.Resultsshowedthatthreecornealcelltypesseededonamnioticmembranegrewwellandhadnormalcellmorphology.Culturedcellsattachedfirmlyonthesurfaceofamnioticmembrane.Cornealepithelialcellsshowedsingularlayerorstratification.Cellboundarieswereformedandtightlyopposed.Cornealendothelialcellsshowedcobblestoneorpolygonalmorphologiccharacteristicsthatappeareduniforminsize.Thecellulararrangementwascompact.Keratocyteselongatedandshowedtriangleordendriticmorphologywithmanyintercellularjointswhichcouldformnetworks.Inconclusion,amnioticmembranehasgoodscaffoldproperty,diffusioneffectandcompatibilitywithcornealcells.Thebasementmembranesideofamnioticmembranefacilitatedthegrowthofcornealepithelialcellsandendothelialcellsandcelljunctionsweretightlydeveloped.Thespongylayerofamnioticmembranefacilitatedthegrowthofkeratocytesandintercellularjointswererich.Amnioticmembraneisanidealbiomaterialforlayeringtissueengineeredcornea.
简介:Tworecentstudieshavediscoveredasurprisinglinkbetweenthreeseeminglydifferentclinicaldiseases:onethatdestroysbonemarrow,anotherthatpreventssundamagefrombeingrepaired,andathirdwhichcauseschildrentoshowsignsofagingextremelyearly.Ithasbeenfoundthatallthreediseasesarecausedbymutationsinthesamegene.Mutationscomeinanumberofdifferentflavors,
简介:
简介:客观:调查运动人工制品的影响在上三维(3D)重建体积和保角的放射疗法计划。方法:能沿着头部尾的方向模仿肺肿瘤的片断运动的一个幽灵被步进马达构造,聚乙烯和土豆的小球。十个不同扫描协议被设置,幽灵的CT数据被使用一台商业GELightSpeed16CT扫描仪获得。CT数据的3D重建被采用GEAdvantageSim6.0系统的显示卷的技术实现。在不同扫描协议的每个目标的重建的体积通过测量工具的3D被测量。因此,在动人的目标和静态的之间的重建体积的相对偏差是坚定的。三维的保角的放射治疗(3DCRT)计划和保角的地与计划系统(TPS)的WiMRT处理为一个静态/动人的目标被创造并且比较。结果:为一个静态的目标,当CT数据与不同程度和片被获得时,在3D重建卷之中没有明显的差别。3D重建体积和3D的外观一个动人的目标的保角的地与静态的的相当不同。最大的相对偏差为与不同扫描协议扫描的一个动人的目标是将近90%。相对偏差在不同目标之中是可变的,关于从-39.8%到89.5%为一个更小的目标并且从-18.4%到20.5%为更大的one.Conclusion:运动人工制品在3D-CRT计划和重建体积上有大效果,它将极大地为一个动人的目标导致弄歪的保角的放射域和假DVH。
简介:Reasonabledenturedesignwillaffectthehealthoforalsupportingtissueandrelatetothesuccessandfailureofprosthodontic.Inordertoinvestigatethestressdistributeoforalsupportingtissueswiththreevariousdesignsunderconditionsofverticalloading,wemeasuredthemodelofthelossoflowerfirstmolarwith3-Dco-ordinatemeasuringapparatus,anddidanaloguedenturedesignwithauto-CADsoft-waresystemincomputer.AllmodelswereanalyzedwithSuper-SAPfiniteelementprogram.Ineachcase,150Nverticalloadingwasappliedonthesecondpremolar,
简介:Havingawiderangeofmotion,thecervicalspineisratherpredisposedtotrau-maandotherdisorderswithseriousconsequences.Themotionofcervicalspineisacomplexonecomposedofmicromotions,includinghorizontaldisplacementsalongallthreeaxesandrotationsaroundthemwithsixfreedomdegreesofmovement.Reportonprecisemeasurementofthethree-dimensionalmotionofthecervicalsectionof
简介:Diastemaoftenoccuredinmaxillarycompletedentureresinbaseintheclinic,although"Lucitone199"hadbeenusedwithmoretensilestrength.Mechanicalex-perimentshaveprovedthehightransversetensilestress(σ_x~+)concentratinginanteri-orteethregionandtheirpalatalsurfaceofmaxillarycompletedentureresinbasewhileitwasexposedtoverticalloadingincenterocclusion.Settingstrengtheningnetinresinbasehasachievedbetterresults.Forevaluatingpurpose,twentykinds
简介:Areaswithhighfrequencyactivitywithintheatriumarethoughttobe'drivers'oftherhythminpatientswithatrialfibrillation(AF)andablationoftheseareasseemstobeaneffectivetherapyine-liminatingDFgradientandrestoringsinusrhythm.ClinicalgroupshaveappliedthetraditionalFFT-basedapproachtogeneratethethree-dimensionaldominantfrequency(3DDF)mapsduringelectro-physiology(EP)proceduresbutliteratureisrestrictedonusingalternativespectralestimationtech-niquesthatcanhaveabetterfrequencyresolutionthatFFT-basedspectralestimation.