简介：Theobjectiveofthisstudyistoinvestigatethehemodynamicsinpatient-specificthoracicaorticaneurysmanddiscussthereasonforformationofaorticplaque.A3-Dimensionalpulsatilebloodflowinthoracicaortawithafusiformaneurysmand3mainbranchedvesselswasstudiednumericallywiththeaverageReynoldsnumberof1399andtheWomersleynumberof19.2.Basedontheclinical2-DimensionalCTslicedata,thepatient-specificgeometrymodelwasconstructedusingmedicalimageprocesssoftware.Un...

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简介：Breastcancerisoneofthemostcommonlydiagnosedmalignanciesandtheleadingcauseofcancerdeathofwomenovertheworld.AlargenumberoffemaleswithbreastcancerinVietnamandotherSoutheastAsian(SEA)countriespresentatanearlyagewithmoreaggressivetumorscomparedwithwomeninAustralia.Despiteexperiencingalowincidencerate,theincreasingincidencerateamongSEAcountriesexceedsthatoftheWesternizedworld.Changesinreproductivefactors,environmentalexposures,andlifestylearethepossiblecausesofthistrend.However,limitedevidenceshowsthatthesefactorsareassociatedwithbreastcancerintheVietnamesepopulation.BreastcancerincidencerateswithinVietnamarenotuniformandappeartobedependentongeographiclocation.FindingsfromthisreviewhaveimportantimplicationsforbreastcancercontrolandtreatmentinVietnam.AgoodunderstandingofthemorphologyofthebreastandthetypeandnatureofbreastcancerspresentinginVietnamisrequiredtofacilitatetheintroductionofaneffectivenationalbreastscreeningprogram.

简介：Newtonian,QuemadaandCassonbloodviscositymodelsareimplementedinordertosimulatetherheologicalbehaviorofbloodunderpulsatingflowconditionsinapatientspecificiliacbifurcation.Theinfluenceoftheappliedbloodconstitutiveequationsismonitoredviathewallshearstress(WSS)distribution,magnitudeandoscillations,non-Newtonianimportancefactors,andviscosityvaluesaccordingtotheshearrate.ThedistributionofWSSonthevascularwallfollowsapatternwhichisindependentoftherheologicalmodelchosen.Ontheotherhand,theWSSmagnitudeandoscillationsaredirectlyrelatedtothebloodconstitutiveequationsappliedandtheshearrate.ItisconcludedthattheNewtonianapproximationissatisfactoryonlyinhighshearandflowrates.Moreover,theNewtonianmodelseemstooverestimatethepossibilityfortheformationofatheroscleroticlesionsoraneurysmsatsitesofthevascularwallwheretheWSSareoscillating.

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简介：食物过敏症是世界范围的一个主要健康问题。桅杆房间在桅杆房间degranulation为需要广泛地被学习的立即的超敏性起一个很重要的作用。在这研究，一条途径被采取在vitro学习敏化的桅杆房间degranulation的特征，它与桅杆房间和动物模型的学习联系了。BALB/c老鼠被几食物变应原分别地使免疫，然后，血和腹桅杆房间在不同时间点被收集。一颗动态决心在桅杆房间和serumalIgE之间被执行。顺序的时间点上的比较分析证明在敏化的BALB/c老鼠在桅杆房间degranulation和IgE抗体titers之间有靠近的巧合。而且，敏化的桅杆房间能在vitro对挑战实现特定的degranulation，有趣，但是仔细，tropomyosins导致了桅杆房间degranulation显示的生气反应。这很类似于在vivo抵抗变应原的IgE。学习在桅杆房间上揭示了一些特征，来自敏化的BALB/c老鼠，在vitro的degranulation。

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简介：WehavedevelopedandtestedchimericT-cellreceptors(TCR)specificforp185HER2.Intheseexperiments,retroviralvectorsexpressingtheN297orN29ξreceptorswereconstructedinpRET6.AmphotropicviralproducercellswereestablishedintheGALV-basedPG13packagingcellline.Ficollpurifiedhumanperipheralbloodlymphocytes(PBL)werevitallytransducedusinganoptimizedprotocolincorporatingactivationwithimmobilizedanti-CD3/anti-CD28monoclonalantibodies,followedbyviralinfectioninthepresenceoffibronectinfragmentCH296.Transducedcellswereco-culturedwithhumantumorcelllinesthatoverexpress(SK-OV-3)orunderexpress(MCF7)p185HER2toassayforantigenspecificimmuneresponses.BothCD4^+andCD8^+T-cellstransducedwiththeN297orN29ξchTCRdemonstratedHER2-specificantigenresponses,asdeterminedbyreleaseofTh1likecytokines,andcellularcytotoxicityassays.OurresultssupportthefeasibilityofadoptiveimmunothempywithgeneticallymodifiedT-cellsexpressingachTCRspecificforp185HER2.

简介：AIM:TOinvestigatetheimmunogenicityofcandidateDNAvaccineagainsthepatitisCvirus(HCV)deliveredbytwoplasmidsexpressingHCVenvelopeprotein1(El)andenvelopeprotein2(E2)antigensrespectivelyandtostudytheeffectofCpGadjuvantonthiscandidatevaccine.METHODS:RecombinantplasmJdsexpressingHCVEIandE2antigensrespectivelywereusedtosimultaneouslyinoculatemicewithorwithoutCpGadjuvant.AntiserawerethencollectedandtJtersofantJ-HCVantibodieswereanalyzedbyELISA.Onemonthafterthelastinjection,animalsweresacrificedtopreparesingle-cellsuspensionofsplenocytes.ThesecellsweresubjectedtoHCVantigenspecificproliferaionassaysandcytokinesecretionassaystoevaluatethecellularimmuneresponsesofthevaccinatedanimals.RESULTS:AntibodyresponsestoHCVEIandE2antigensweredetectedinvaccinatedanimals.AnimalsreceivingCpGadjuvanthadslightlylowertitersofanti-HCVantibodiesinthesera,whilethesplenocytesfromtheseanimalsshowedhigherHCV-antigenspecificproliferation.Analysisofcytokinesecretionfromthesplenocyteswasconsistentwiththeaboveresults.Whilenoantigen-specificIL-4secretionwasdetectedforallvaccinatedanimals,HCVantigen-specificINF-γ,secretionwasdetectedforthesplenocytesofvaccinatedanimals.CpGadjuvantenhancedthesecretionofINF-γ,butdidnotchangetheprofileofIL-4secretion.CONCLUSION:VaccinationofmicewithplasmidsencodingHCVE1andE2antigensinduceshumoralandcellularimmuneresponses.CpGadjuvantsignificantlyenhancesthecellularimmuneresponse.

简介：Objective:Toinvestigatethebindingcharacteristicsofendothelialcell(EC)withLPSfreefromtheparticipationofserumfactors.Methods:LaserconfocalmicroscopewasemployedintheobservationofthebindingofECwithFITC-LPS.TheKDandthebindingsitesofeachECwerecalculatedbyradioligandbindingassayofreceptors(RBA)using[3H]-LPS.Results:ThebindingofECwithLPSwassaturable,timeandconcentrationdependentanditcouldbecompetedwithoverdosedLPSofthesametype.Thefluorescencemainlydistributedincytoplasm,especiallynearthenucleus,whichcouldalsobestained.Conclusions:TheremightbesomespecificLPSbindingsitesexistingonEcsandLPScouldfunctionintracellularily.

简介：含水土层的特定的产量(Sy)的评价在水资源管理是很重要的。在这研究，一个试验性的排水方法被开发决定Platte河山谷的冲积含水土层的特定的产量，内布拉斯加，美国。有连续间隔深度的沉积核心的集合用直接的推技术被收集到塑料试管然后带去实验室。在Sy实验期间，那些沉积核心被垂直地把他们放在一辆大水坦克重新浸透。Sy被严肃的力量在一个方括号从沉积排干的水决定。我们Sy的价值与在测试地点之间的间隔深度为Sy在每个地点和可变性主要与深度改变了的结果表演也被观察。在Sy的这空间异质可能源于谷物尺寸的变化，谷物形状，排序并且在有间隔深度的不同核心的沉积的压缩。为所有沉积核心的Sy从0.01～0.18，吝啬的价值是0.08譨?譨吗？

简介：AbstractObjective:It has recently been shown that the melanoma antigen gene (MAGE) family is expressed in various tumor cell lines but silent in normal tissues, except germ cell lines. Mageb4, a member of the MAGE family, is highly expressed in the testis and homologous in humans and mice. Whole-exome sequencing studies have identified Mageb4 as a possible X-linked cause of inherited male infertility. However, the function of Mageb4 protein remains largely unknown.Methods:Using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 technology, we generated a Mageb4 knockout mouse model (Mageb4-/Y) to explore the role of this gene in spermatogenesis.Results:First, immunostaining of testicular cells showed that Mageb4 is localized in the cytoplasm of spermatogonia. Second, Mageb4-/Y male mice displayed significant increases in apoptosis. However, Mageb4-/Y male mice showed normal fertility, including normal sperm concentration, sperm motility, and testicular and epididymal histology.Conclusions:These findings suggest that, despite testis-exclusive expression, Mageb4 is dispensable for mouse spermatogenesis. Future research should focus on the role of this gene in apoptosis, aiming to provide clinical guidance regarding male infertility.

简介：CMV特定的免疫为人的cytomegalovirus(HCMV)的控制是必要的感染。干细胞移植在hemopoietic系统的疾病的一个范围的管理广泛地被使用。病人们在早posttransplant时期深刻地是immunosuppressed，并且cytomegalovirus(CMV)的复活仍然是病态和死亡的一个重要原因。CMV特定的CD8+T房间克隆的采纳转移被显示了减少病毒的复活的率；然而，为采纳转移的房间的前vivo生产是集中、昂贵的劳动。我们这里用CMV特定的epitope肽报导一个修改肽刺激方法为CMV特定的CTL的产生刺激PBMC。这个方法允许CMV特定的CTL的有效扩大并且从一件单个血样品为FACS分析提供很多房间。显著地，它完成允许容易要比较并且顺序评估了的不同个人的数据的CD8+T房间染色的tetramer的高频率。因此，这条途径为潜在的采纳免疫疗法扩展并且选择高特性的限制HLA的CMV-pp65-reactiveT房间线。

简介：由使用父母，Kunmingxiaobaigu，有从云南的冷忍耐的一个陆地赛跑变化，中国，和Towada，一个寒冷敏感的变化作为测试材料在日本，以及theirF_8和F_9RIL子孙繁殖了，在在在四不同高度(环境)下面的2002and2003的孕穗期的米饭的冷忍耐，并且在小穗状花小穗富饶和单个植物的特定的小穗状花小穗富饶(到在每圆锥花序的顶的三个主要分支的the5th小穗状花小穗的从3rd的九小穗状花小穗的小穗状花小穗富饶)之间的英国管关系被分析。数据从0.7364～0.9011与范围揭示了在小穗状花小穗富饶和特定的小穗状花小穗富饶之间的一种重要英国管关系。在冷压力环境下面，没有冷应力，然而，相关系数比那些高。在另一方面，panicleexsertion被相关到小穗状花小穗富饶和特定的小穗状花小穗富饶。而且，遗传分析建议特定的小穗状花小穗富饶能作为鉴定指示物代替小穗状花小穗富饶，当时圆锥花序使突出离子能在米饭在孕穗期被用作冷忍耐鉴定的部分指示物。

简介：ThespecificsignofReynoldsstressintheboundarylayeronaflatplateatzeroincidenceisnewlyinterpretedinpresentpaperbasedonthetheoryofvortex-inducedvortex.Itavoidssomeproblemsappearedinatraditionalexplanation,onthebasisofrelationshipbetweenmeanandfluctuatingflowsduetothetransportofmomentum.Throughtheanalysisoflocalflowfieldintheimmediateneighborhoodofwall,thecharacteristicsofReynoldsstressareidentifiedthroughintroducingturbulence-inducedsmall-scalestreamwiseeddiesabovetheflatplate.ThepositiveReynoldsstressistheoreticallyverified.AndsuchnewinterpretationillustratesthatthegenerationofReynoldsstress,aswellasfluctuatingvelocity,isintrinsicallyindependentofthemeanflow.Butitsspecificsignwouldbedeterminedbythemeanflowduetotheinertialforces.Otherfeatures,suchastheintensityrelationshipamongthreecomponentsoffluctuatingvelocity,arealsopresented.

简介：XADisanapoptosisspecificDNaseinXenopusandcancutthelinker-DNAbetweennucleosomesduringapoptosisinXenopuslaeviseggextractinducedbycytochromec.XADismostlikelytobethehomologuetoDFF40inhumans.TheactivityofDFF40canbeinhibitedbyDFF45.WereportmolecularcloningandidentificationofanXADinhibitor,IXAD(inhibitorofXAD),inXenopuseggs.WeclonedthecompletecDNAoftheDFF45homologue,IXAD.ThereisaspecificDEVDsequenceinitsN-terminal,whichserves

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简介：GABAtransporter1(GAT1)takesimportantrolesinmultiplephysiologicalprocessesthroughtheuptakeandreleaseofGABA,buttheregulationofGAT1geneexpressionindifferenttissuesisrarelyknown.Toaddressthequestion,first,5'RapidamplificationofcDNAend(RACE)wasusedtodetermineGAT1transcriptionalstartingsitesinneonatalmousecerebralcortexandintestine,adultmousebrainandadultrattestis.Theproductsof5'RACEwereconfirmedbyDNAsequencing.WefoundthatthetranscriptofGAT1inneonatalmousecerebralcortexandadultmousebrainstartsatthesamesite(insideofexon1),whileinmouseintestine,GAT1startstranscriptioninintron1,andinrattestis,thetranscriptofGAT1hasanadditionaluntranslationexontothe5'direction.

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简介：ThemaintechnicalbackgroundsandrequirementsareintroducedwithregardtoearthquakegroundmotiondesignparametersinseveraldomesticandAmericanstandards,codesandguidesinvolvedintheseismicanalysisanddesignactivitiesofnuclearpowerplantsinChina.Basedontheresearchresultsfromsiteseismicsafetyevaluationofdomesticnuclearpowerplantprojectsinthelastyears,characteristicsanddifferencesofsitespecificdesignspectraareanalyzedincomparisonwithstandardresponsespectra,andthesuitabilityofstandardresponsespectrafordomesticnuclearpowerplantprojectsisdiscussed.