简介：目的:探讨单侧内直肌截除术治疗儿童残余性和复发性外斜视的有效性及安全性，评价其在不同外斜视类型及不同初次手术方式应用的差异性。方法：回顾性病例系列研究。收集2009-01/2013-02在山东大学附属山东省立医院行单侧内直肌截除术治疗的残余性和复发性外斜视连续性病例48例48眼，观察术后第Id，6wk以及末次随访（术后6~32mo)时患儿眼位、眼位非共同性、融合功能及立体视锐度情况。结果:术后第Id手术正位率为83%(40/48)，欠矫率为4%(2/48)，过矫率为13%(6/48);术后第6wk手术正位率为81%(39/48)，欠矫率为13%(6/48)，过矫率为6%(3/48);末次随访时正位率为75%(36/48)，欠矫率为25%(12/48)，无1例过矫。不同初次手术方式和不同外斜视类型患儿末次随访时手术正位率的差异均无统计学意义（P=0.168、0.50)。术后所有病例均未出现眼球运动非共同性和眼球外转受限。结论:单侧内直肌截除术是治疗儿童残余性和复发性外斜视的安全有效术式，其在不同外斜视类型及不同初次手术方式应用的疗效无差异。

简介：目的评价小切口白内障囊外摘除联合人工晶体植入术对高度近视并发性白内障的临床疗效。方法对我院2006年4月～2010年5月期间实施小切口非超声乳化白内障囊外摘除术治疗的23例（28眼）高度近视（眼轴〉26mm）并发性白内障患者病案资料进行回顾性分析。结果所有患者均顺利完成手术,术中3眼晶状体后囊膜破裂,1眼术后发生视网膜脱离,术后3d最佳矫正视力达0.3以上者21眼（75%）,术后1个月最佳矫正视力达0.3以上者23眼（82%）。结论小切口白内障囊外摘除术治疗高度近视并发性白内障,术中及术后并发症少,术后视力恢复好,对手术设备要求不高,是基层医院治疗高度近视并发性白内障的理想术式。

简介：目的探讨总结小切口白内障囊外摘除联合人工晶体植入术的临床疗效及在防盲工作中的价值.方法对本院2011-2012年200例行小切口白内障囊外摘除联合人工晶体植入术,术后患者的视力及术后并发症进行临床分析及总结.结果与术前视力比较,视力明显提高,术后3天,3个月视力达0.5以上55.1％,75.7％,术后角膜散光与术前差异不大,手术操作简单,并发症少.结论小切口白内障囊外摘除联合人工晶体植入术具有切口小,散光小,术后复明效果好,操作简单,手术经费低的优点,是白内障超声乳化术的有效补充,适合在基层医院复明工程中推广.

简介：目的：探讨舌状截囊方法在过熟期白内障囊外摘除联合人工晶状体植入术的临床效果。方法：对380眼过熟期白内障囊外摘除联合人工晶状体植入术中采用舌状截囊方法,术后观察及随访其疗效。结果：所有患眼术后视力均有不同程度提高,视力恢复情况：术后1d,0.1以下85眼（22.4%）,0.1-0.3者162眼（42.6%）,0.4-0.6者87眼（22.9%）,0.6以上者46眼（12.1%）。术后1mo0.1以下33眼（8.7%）,0.1-0.3者152眼（40.0%）,0.4-0.6者117眼（30.8%）,0.6以上者78眼（20.5%）。术中术后并发症：术中悬韧带断离16眼（4.2%）,后囊膜破裂25眼（6.6%）,角膜水肿53眼（13.9%）,人工晶状体偏位12眼（3.2%）。结论：舌状截囊方法容易掌握,手术的安全性更好,更适合在援非过熟期白内障囊外摘除联合人工晶状体植入术中的应用。

简介：AIM:ToinvestigatetheroleofRho-associatedproteinkinase(ROCK)inhibitor,Y27632,inmediatingtheproductionofextracellularmatrix(ECM)componentsincludingfibronectin,matrixmetallo-proteinase-2(MMP-2)andtypeIcollagenasinducedbyconnectivetissuegrowthfactor(CTGF)ortransforminggrowthfactor-β(TGF-β)inahumanretinalpigmentepithelialcellline,ARPE-19.METHODS:TheeffectofY27632ontheCTGForTGF-βinducedphenotypeinARPE-19cellswasmeasuredwithimmunocytochemistryasthechangeinF-actin.ARPE-19cellsweretreatedwithCTGF(1,10,100ng/mL)andTGF-β(10ng/mL)inserumfreemedia,andanalyzedforfibronectin,laminin,andMMP-2andtypeIcollagenbyRT-qPCRandimmunocytochemistry.CellswerealsopretreatedwithanROCKinhibitor,Y27632,toanalyzethesignalingcontributingtoECMproduction.·RESULTS:TreatmentofARPE-19cellsinculturewithTGF-βorCTGFinducedanECMchangefromacobblestonemorphologytoamoreelongatedswirlpatternindicatingamesenchymalphenotype.RT-qPCRanalysisanddifferentgeneexpressionanalysisdemonstratedanupregulationinexpressionofgenesassociatedwithcytoskeletalstructureandmotility.CTGForTGF-βsignificantlyincreasedexpressionoffibronectinmRNA(P=0.006,P=0.003respectively),lamininmRNA(P=0.006,P=0.005),MMP-2mRNA(P=0.006,P=0.001),COL1A1mRNA(P=0.001,P=0.001),COL1A2mRNA(P=0.001,P=0.001).PreincubationofARPE-19withY27632(10mmol/L)significantlypreventedCTGForTGF-βinducedfibronectin(P=0.005,P=0.003respectively),MMP-2(P=0.003,P=0.002),COL1A1(P=0.006,P=0.003),andCOL1A2(P=0.006,P=0.004)geneexpression,butnotlaminin(P=0.375,P=0.516).CONCLUSION:OurstudydemonstratedthatbothTGF-βandCTGFupregulatetheexpressionofECMcomponentsincludingfibronectin,laminin,MMP-2andtypeIcollagenbyactivatingtheRhoA/ROCKsignalingpathway.Duringthisprocess,ARPE-19cellswereshowntochangefromanepithelialtoamesenchymalphenotypeinvi

简介：AIM:Topresenttheoutcomeofmodifiedgridlaserphotocoagulation(GLP)indiffusediabeticmacularedema(DDME)ineyeswithoutextrafovealand/orvitreofovealtraction.METHODS:InclusioncriteriafortheretrospectivestudywereDDMEeyesofpatientswithtypeⅡdiabetesmellitusthathad≥4monthsoffollow-upfollowingGLP.Onlyoneeyeperpatientwasanalyzed.Using3-Dspectral-domainopticalcoherencetomography(3-DSDOCT),eyesthathadeitherextrafovealorvitreofovealtraction,orhadbeenpreviouslytreatedbyanintravitrealmedication(s)wereexcluded.TreatedDDMEeyesweredividedinto4groups:A)'Classic'DDMEthatinvolvedthecentralmacula;B)edemadidnotinvolvethemacularcenter;C)eyesassociatedwithcentralepiretinalmembrane(ERM);D)DDMEthatwasassociatedwithmacularcapillarydropout≥2disc-diameter(DD).RESULTS:GLPoutcomein35DDMEeyesafter4-24(mean,13.1±6.9)monthswasasfollows:GroupA)18eyeswith'classic'DDME.Followingoneor2(mean,1.2)GLPtreatments,best-correctedvisualacuity(BCVA)improvedby1-2Snellenlinesin44.4%(8/18)ofeyes,andworsenedby1linein11.1%(2/18).Centralmacularthickness(CMT)improvedby7%-49%(mean,26.6%)in77.8%(14/18)ofeyes.CausesofCMTworsening(n=4)werecommonlyexplainable,predominantly(n=3)associatedwithemergenceofextrafovealtraction,5-9monthspost-GLP.GroupB)GLP(s)inDDMEthatdidnotinvolvethemacularcenter(n=6)resultedinimprovedBCVAby1-2linesin2eyes.However,thecentralmaculabecameinvolvedintheedemaprocessaftertheGLPin3(50%)eyes,associatedwithanemergenceofextrafovealtractioninoneoftheseeyes4monthsfollowingtheGLP.GroupC)GLPfailedinall5eyesassociatedwithcentralERM.GroupD)GLPwasofpartialbenefitin2of6treatedeyeswithmacularcapillarydropout≥2DD.CONCLUSION:EyeswithDDMEthatinvolvedthemacularcenterwerefoundtoachievefavourableoutcomesafterGLP(s)duringmid-termfollow-up,unlesscomplicatedpre-GLPorpost-GLPbyvltreoretinalinterfaceabnormalities,oftenextrafovealtra

简介：目的探讨并总结小切口白内障囊外摘除联合人工晶状体植入术治疗晶状体溶解性青光眼的临床疗效。方法回顾分析2011年3月至2016年5月间在我科确诊并行小切口白内障囊外摘除联合人工晶状体植入术的30例30眼晶状体溶解性青光眼,观察并记录其视力、眼压及并发症等变化情况。结果30例患者手术均顺利进行,术中未出现任何并发症,术后3个月随访时视力恢复良好,有6眼视力在0.1-0.5,占20.0%,24眼视力在0.6-0.8,占80.0%,矫正视力在1.0及其以上的有6眼,占20.0%;所有患者术后随访时眼压均在正常范围;术后均无严重影响眼压及视力的并发症出现。结论小切口白内障囊外摘除联合人工晶状体植入术治疗晶状体溶解性青光眼具有良好的临床效果,值得在基层医院推广。

简介：目的探讨小切口白内障囊外摘除人工晶状体植入术治疗高度近视眼并发性白内障的临床效果，方法同顾性分析2004年6月-2007年6月在我科行小切口白内障囊外摘除人工晶状体植入术的高度近视眼并发性白内障的患者48例，观察术中、术后并发症及术后视力恢复情况。结果所有患者均顺利完成小切口自内障囊外摘除人工晶状体植入术，术中两例患者晶状体后囊膜破裂，术后3月最佳矫正视力0．1-0．3者6眼，0．3-0．8者30眼，〉0．8者12眼，术中、术后无其它并发症发生。结论小切口白内障囊外摘除人工晶状体植入术治疗高度近视眼并发性白内障，术中、术后并发症少，术后患跟视力恢复好，且对手术设备要求不高，足适应于广大基层医院的最佳手术方式之一。

简介：AIM:ToInvestigatetheeffectsoftransforminggrowthfactorβ2(TGF-β2)andconnectivetissuegrowthfactor(CTGF)ontransdifferentiationofhumanlensepithelialcells(HLECs)culturedinvitroandsynthesisofextracellularmatrix(ECM).METHODS:HLECsweretreatedwithTGF-β2(0,0.5,1.0,5,10μg/L)andCTGF(0,15,30,60,100μg/L)fordifferenttimes(0,24,48,72h)invitroandtheexpressionofα-smoothmuscleactin(α-SMA),themaincomponentoftheextracellularmatrixtypeⅠcollagen(Col-1)andfibronectin(Fn)weremeasuredbyusingreal-timepolymerasechainreaction(PCR)andwestern-blot.RESULTS:TGF-β2andCTGFsignificantlyincreasedexpressionofα-SMAmRNAandprotein(P<0.05,P<0.001),FnmRNAandprotein(P<0.001),Col-1mRNAandprotein(P<0.001).TGF-β2couldinduceHLECsexpressionofCTGFmRNAandproteinindosedependentmanner(P<0.05,P<0.001).TGF-β2andCTGFcouldinduceHLECstoexpressα-SMA,FnandCol-1intime-dependentmanner.EachtimeofTGF-β2andCTGFinducedHELCsexpressionofα-SMA,Fn,Col-1mRNAandproteinwassignificantincreasecomparedwithcontrol(P<0.05,P<0.001).CONCLUSION:TGF-β2andCTGFcouldinduceHLECsepithelialmesenchymaltransitionandECMsynthesis.