简介：Outerdensefibersarespecificsubcellularcomponentsofmammalspermflagella,apartfromtheaxonemeanditsassociatedproteins.Asoneoftheouterdensefiberscomponents,ODF3isessentialfortheformationofmammalspermflagella.Inthepresentstudy,weisolatedtheOdf3gene,1033bpinlength,fromZhikongscallopChlamysfarreri,whichwasnamedasCf-Odf3.ItwaspredictedthatCf-Odf3encodesaproteinof240aminoacidswhichcontains5discontinuousPro-Gly-Prorepeats.TheCf-Odf3transcriptsweredetectedinbothtestisandovaryofC.farreriatvariousstagesthroughqRT-PCR.TheCf-ODF3proteinsynthesizedbyprokaryoteswaspurifiedusingNi2+-NTAaffinitychromatographyandusedtoproduceitspolyclonalantibody.TheantibodyproductwaseffectiveandspecificaswasexaminedbyWesternblotting.TheCf-Odf3transcriptandCf-ODF3proteinwerelocatedinintragonadalsomaticcells(ISCs),spermatogoniaandspermatocytesofC.farreritestes,whilenotranscriptionofCf-Odf3andsynthesisofCf-ODF3weredetectedinspermatidsandspermatozoa.ThelocationofOdf3expressionisdifferentfromthatofvertebrateswhereODF3issynthesizedspecificallyinspermatidsandspermflagella.Moreover,theCf-Odf3expressionwasalsodetectedinISCs,oogoniaandoocytesofthescallopovaries.OurfindingrevealedadifferentcharacteristicofOdf3expressionbetweenscallopandvertebrates,whichimpliedthatCf-Odf3playedaroleinthegametogenesisofC.farreri.

简介：EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.

简介：利用FNL再分析资料,统计2008-2012年入海发展江淮气旋并根据气旋不同深厚程度及季节特征分为：暖季深厚型、暖季浅薄型、冬季浅薄型和春初底层型.各类气旋的统计及合成分析表明四类气旋入海基本特征为：入海路径可分为东路和东北路;冬季与初春气旋入海发展增强幅度大于暖季;不同深厚气旋入海后均有下垫面摩擦力减小近海面风力增强,大风区扩大且由气旋偏东位置向东南偏移;暖季气旋入海降水强度增幅明显,并与气旋深厚程度成正比,冬季及春初气旋入海后降水增幅小,春初气旋后部有零散强降水.对入海发展机制的合成诊断显示,气旋中凝结潜热释放对暖季气旋起重要作用,并与气旋深厚程度成正比,对冬季气旋也有正贡献,但对春初底层型气旋无明显作用.春初底层型对海面动力热力影响更敏感,入海后正涡度区的垂直伸展较其它型更显著.而有利于气旋加深的上空辐散中心位置高度与气旋的深厚程度成正比.气旋入海发展中环境因子分析显示,下垫面非绝热加热对冬季和初春气旋作用显著,对暖季气旋影响不明显.高空急流动量下传与下垫面摩擦减弱促使各类气旋增强.湿位涡对暖季气旋有重要正贡献,对深厚气旋作用更强.冬季和初春风场的惯性稳定度和切变稳定度的共同作用有利于气旋增强.1000hPa上湿斜压项MPV2显示的气旋区域温湿锋区位置及强度与入海气旋雨区及雨强对应较好,具有显著指示性.

简介：TiO2filmswereformedonmetallictitaniumsubstratesbytheanodicoxidationmethodinH2SO4solutionunderthe80VD.C..PhasecomponentandmicrostructurewerecharacterizedbyX-raydiffraction(XRD)andscanningelectronmicroscopy(SEM).Watercontactanglesontitaniumoxidefilmsurfaceweremeasuredunderbothdarkandsunlightilluminationconditions.Corrosiontestswerecarriedoutinseawaterunderdifferentilluminationconditionsbyelectrochemistryimpedancespectrum(EIS)andpolarizationcurves.TheresultshowedthattheTiO2filmpreparedbytheanodicoxidationmethodwasanatasewithauniformstructureandwithoutobviousporesorcracksonitssurface.Theaveragewatercontactangleofthefilmwas116.4?indark,incontrasttoanangleof42.7?undertheUVilluminationfor2hours,whichdemonstratesgoodhydrophobicproperty.Theanti-corrosionbehavioroftheTiO2filmwasdecliningwiththeextendedimmersiontime.Underdarkconditions,however,thehydrophobicTiO2filmretardedthewaterinfiltratingintothesubstrate.Theimpedancechangedslowlyandthecorrosioncurrentdensitywas2ordersofmagnitudelowerthanthatwiththefilmilluminatedbysunlight.AllofthosementionedaboveindicatethattheTiO2filmpossessesmuchbetterperformanceunderdarkcondition,anditcanbeappliedasanengineeringmaterialunderdarkseawaterenvironment.

简介：为弥补测高数据在近海区域因为编辑准则被剔除而导致近海测量数据质量不高的缺点,利用5参数模型的两种形式和Ice-2模型对Jason-2卫星在中国南海部分海域的海洋回波波形进行了波形重跟踪的研究,并选择20个海洋回波波形,依据最小二乘原理进行计算、分析、对比,发现5参数模型的指数形式的结果和Ice-2模型结果非常接近.研究结果表明,在开阔的海域,对Jason-2波形进行3种方法波形重跟踪时,可以优先考虑Ice-2模型.波形重跟踪技术能够提高卫星测高数据利用率,为科学研究提供可靠的数据保障.

简介：3种鞘脂类代谢产物N-2′-羟基十六碳酰基-1-O-β-D-吡喃葡萄糖基-9-甲基-4E-8E-十八碳二烯-1-醇（A）、N-2′-羟基-3′E-十八碳烯酰基-1-O-β-D-吡喃葡萄糖基-9-甲基-4E，8E-十八碳二烯-1-醇（B）和N-十六碳酰基二氢鞘氨醇（C）分别产生于来源于中国南海的2株海洋真菌（菌株编号为1924和3893），通过2DNMR、MS等方法测定了它们的结构。这是首次从南海红树内生真菌分离得到的3种鞘脂类代谢产物。

简介：本文详细介绍了SIS海冰模式中引进两种盐度参数化方案即等盐度方案和盐度廓线方案对海冰模拟所存在的差异。利用盐度廓线方案导出的表征盐度与海冰温度间关系的方程比等盐度方案多出一项，将定义为盐度差异项。盐度差异项对海冰厚度的热力作用表现为：在海冰厚度增长季节（11月到次年5月），盐度差异项通过升高海冰内部温度，抑制海冰增长；在消融的第一阶段（6．8月），盐度差异项通过升高海冰内部温度加快海冰消融；在消融的第二阶段（9．10月），盐度差异项通过降低海冰内部的温度抑制海冰消融。但尺度分析表明，盐度差异项要比方程中队海冰温度作用最大项小1．2个量级，如果采用一级近似，可以略去盐度差异项，因此盐度差异项对海冰增长和消融影响很小。同时利用冰洋耦合模式（ModularOceanModel，MOM4），分别采用两种盐度参数化方案模拟北极海冰厚度和海冰密集度的季节性变化，模拟结果也表明两种方案模拟得到的海冰厚度和海冰密集度的季节性变化相差甚小。

简介：AbundantevidencesofhighersealevelsfromJiangsuandFujiancoastshaveprovedamarinetransgressioneventduring30–40kaBP,suggestingthattherewasastagewithhighsealevelandawarmclimatewhenicesheetsshrankintheNorthernHemisphere.Thedurationof30–40kaBPspannedaperiodinthelateMarineIsotopeStage3(MIS3)andwasinnatureaninterstadialepochduringtheLastGlacialperiodoftheQuaternary.Differentfromtheglacialperiodwithacoldclimate,thismarinetransgressionconsideredasapenultimatehighersealevelduringtheQuaternaryremainsapuzzlethatwhytheevidenceiscontrarytotheQuaternaryglacialtheory.Itisimportanttounderstandsealevelrisefortheseareassensitivelyrespondingtotheglobalchangesinthefuture.Torecognizethekeyissuesonsealevelchanges,theeustaticsealevel(H_S)wasdefinedastheglaciation-climateforcedsealevels,andtherelativesealevelchange(H_R)wasdefinedasthatasealevelrecordwaspreservedinsedimentthatexperiencedmultiplesecondaryactionsoflandandseaeffects.Onthebasisasdefinedabove,weconstructedmulti-levelmodelsofclimate-drivenglacio-eustaticchangesandland-seasystems.Byintegratingdatasetsfromeightboreholecoresandprescribingtheboundaryconditions,wesimulatedthechangesofHSandHRintheEastChinaSeaandsouthernYellowSeaareasinthelateMIS3.Themarinetransgressionstratafromtheboreholecoredatawasidentifiedatca.30mbelowpresentsealevelasaresultofthecollectiveinfluenceoficemeltingwater,neotectonicsubsidence,sedimentcompactionandterrestrialsedimentfillingsinceca.35kaago,whereasthesimulatedrelativesea-levelsturnedouttobe–26.3––29.9ma.s.l.Thesmallerrorinvolvedinthesimulationresultsof±(2.5–4.5)mdemonstratedthecredibilityoftheresults.OurresultsindicatedthatsealevelchangeinthelateMIS3wasdominatedbyglacialeffects,inwhichtheeustaticsea-levelwasbetween–19.2�

简介：FattyacidcompositionsoftwostrainsofmarinediatomsNitzschiaclosterium(MACCB222)andChaetocerosgracilis(MACCB13)havebeenexamined.ThemicroalgaehavebeengrownatdifferentinitialNaNO3concentrations(75,375,750,1125,1500,1875mg/L)andharvestedatthelateexponentialphase.TheresultsbyonefactoranalysisofvarianceshowthattheNaNO3concentrationshavenosignificantinfluenceontherelativegrowthrateoftwostrainsofmarinediatoms;theinfluenceofNaNO3concentrationsonthefattyacidsdiffersfromspeciestospecies.Themajorfattyacidsoftwodiatomstrainsare14:0,16:0,16:ln-7and20:5n-3(EPA),B13alsohashighpercentageof20:4n-6(6.0～9.1%).

简介：CarboncycleisconnectedwiththemostimportantenvironmentalissueofGlobalChange.Asoneofthemajorcarbonreservoirs,oceansplayanimportantpartinthecarboncycle.Inrecentyears,ironseemstogiveusagoodnewsthatoceanicironfertilizationcouldstimulatebiologicalproduc-tivityasCO2sinkofhuman-producedCO2.Oceanicironfertilizationexperimentshaveverifiedthataddingironintohighnutrientlowchlorophyll(HNLC)seawaterscanincreasephytoplanktonproductionandexportorganiccarbon,andhenceincreasecarbonsinkofanthropogenicCO2,toreduceglobalwarming.Insixtydays,theexportorganiccarboncouldreach10000timesforaddingironbymodelpredictionandinsituexperiment,I.e.TheatmosphericCO2uptakeandinorganiccarbondrawdowninupperseawatersalsohavethesamemagnitude.Therefore,oceanicironfertilizationisoneofthestrate-giesforincreasingcarbonsinkofanthropogenicCO2.Thepaperisfocusedontheironfertilization,es-peciallyinsituoceanironexperimentsinorderthatthefutureresearchismoreefficient.

简介：Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.

简介：基于广州及深圳海岛海岸带区域的1：2000航空摄影测量项目,对比分析A3航摄仪在有、无地面像控点情况下的空中三角测量精度.结果表明,在无地面像控点的情况下,A3仍可满足海岛海岸带区域大比例尺成图的精度要求,且航摄效率高、成图周期短.本研究可为A3在海岛海岸带区域的应用提供参考.

简介：随着卫星遥感技术的不断发展,高分辨率卫星影像逐渐应用到水深遥感反演领域.利用Worldview-2高分辨率卫星数据和电子海图数据,基于双波段比值法,反演获得实验区域20m以浅的水深.实验表明,Worldview-2等高分辨率多光谱卫星数据,具有一定反演浅水水深的能力,但在5m以浅的水域反演误差较大;双波段比值法,这种半经验半理论的模型,在水深遥感反演中具有更好的适用性;对比了一次线性、二次多项式、指数、对数等拟合方法,发现对数拟合的方法获取绝对水深,其精度相对其他方法更高.

简介：有不同规模的很多海底的山崩在Liwan3-1气体地的海底的管道线路的峡谷区域被识别了。仍然有许多机会，海底的斜坡失败将发生，并且下列集体运动将介绍大风险给海底的管道。鉴于这，一个数字预言方法基于Eulerian-Eulerian二阶段的流动模型被介绍模仿潜在的海底的山崩的集体运动。滑动土壤和周围的水被Herschel-Bulkley流变学模型和牛顿的液体模型分别地模仿。骚乱用k-模型被模仿。与两个相比实验数据并且是结果，二阶段的流动模型显示出好精确性，并且它的结果是更多关门到实际状况；在土壤和周围的水之间的动态联合能有效地被模仿，hydroplaning和头分开的现象能被获得。最后，潜在的海底的山崩的土壤运动作为一个例子被模仿，根据在峡谷区域的地震侧面。结果证明hydroplaning发生在运动过程期间。二阶段的流动模型计算的刺杀距离是877m，它是27.1%比是大结果。然而，土壤的山峰前面速度是相对的小，与8.32m/s的最大的价值。是有一个简单、快速的过程的节目能被用于初步的评估，当二阶段的流动模型为一个精确评价是更适当的时。

简介：一个方便、有效的过程被tosylation和硅石胶化在这研究支持的随后的β-消除为酒精的变换开发到石蜡。有在在0℃的pyridine的p-toluenesulfonyl氯化物的白酒的处理负担得起在房间温度在dichloromethane或氯仿与硅石胶化经历β-消除的tosylates，产出有高生产率的石蜡。

简介：TheDiverGasRecoverySystem(DGRS)isanimportantaccessoryequipmentofthe300mSaturationDiveSystem.Thesystemfacilitatestherecyclingofthebreathinggasesfordivers,therefore,ithasgreateconomicbenefits.InApril1995,a300munmannedtestanda100mmannedtestontheHY2031DGRSwerecarriedoutsuccessively.Tenmonthslater,anothermannedtestontheHY2032DGRS

简介：AninvivofluorescencediscriminationtechniqueforphytoplanktonpopulationwasdevelopedbyusingWaveletpackettransform,clusteranalysisandnon-negativeleastsquares.Thetechniquewasusedtoanalyzewatersamplesfromdifferentsearegions.Forsimulativemixtures,whendominantspeciesaccountfor60%,70%,80%,90%atthedivisionlevel,thecorrectdiscriminationratios(CDRs)are83.0%,99.1%,99.7%and99.9%withtherelativecontentsof58.5%,68.4%,77.7%and86.3%,respectively;whenthealgaedominanceare60%,70%,80%,90%,100%atthegenuslevel,theCDRsare86.1%,94.9%,95.2%,96.8%and96.7%,respectively.Forlaboratorymixtures,theCDRsare88.1%and78.4%atthedivisionandgenuslevel,respectively.Forfieldsamples,theCDRswere91.7%and80%atthedivisionandgenuslevel,respectively(mesocosmexperiments),andtheCDRswere100%and66.7%atthedivisionandgenuslevel,respectively(JiaozhouBay).ThefluorometrictechniquewasusedtoestimatethephytoplanktoncommunitycompositionandrelativeabundanceofdifferentclassesfortheApril2010cruiseintheYellowSeawiththeresultsagreeingwiththoseinpublishedpapersbyotherauthors.

简介：在本研究中，我们首次通过基因组上游第一次步移克隆到了雨生红球藻中crtO基因的3个不同大小的5’上游侧翼序列1．1kb，1．9kb和2．2kb。利用生物信息学方法分别预测并比较了这3个不同大小的5’上游侧翼序列，结果发现，三者具有与高等植物相类似的顺式作用元件，如脱落酸反应元件（ABRE）、干燥或低温反应元件（DRE／C-repeat）、几种光反应元件（G1-box,GAG-motif，l-boxandATC—motif）、热激反应元件（HSE）、机械伤害反应元件（WUN-motif）、生长素反应元件（TGA-element）、茉莉酸甲酯反应元件（TGACG-element）和反式作用因子MYB蛋白的结合位点（MBSandMRE）等等，但是三者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中crtO基因调控方式的多样化。

简介：TostudytheTaiwanStrait(TS),anunusualseaarea,thenumericalmodelinmarginalseasofChinaisusedtosimulateandanalyzethetidalwavemotioninthestrait.ThenumericalmodelingexperimentsreproducetheamphidromicsystemoftheM2tideinthesouthendoftheTaiwanstrait,andconsequentlyconfirmtheexistenceofthedegenerateamphidromicsystem.Onthisbasis,furtherdiscussionisconductedontheM2systemanditsformationmechanism.ItcanbeconcludedthatthetidalwavesoftheTSisconsistedoftheprogressingwavefromthenorthentranceandthedegenerateamphidromicsystemfromthesouthentrance,inwhichtheprogressingwavefromthenorthentrancedominatesthetidalwavemotioninthestrait.Exceptfortheconvergenteffectcausedbythelandformandboundary,thedegenerateamphidromicsystemproducedinthesouthofthestraitisanotherimportantfactorforthefollowingphenomena:thelargetidalrangeinthemiddleofthestrait,theconcentrativezoneofco-amplitudeandco-phaselineinthesouthofthestrait.ThedegenerateamphidromicsystemismainlyproducedbytheincidentPacificOceantidalwavefromtheLuzonstraitandtheactionbytheshorelineandlandform.Thepositionoftheamphidromicpointiscompelledtomovetowardsouthwestuntildegeneratingbythepowerfulprogressingwavefromthenorthentrance.

简介：测定了相手蟹属（Sesarma）红螯相手蟹（S．haematocheir）和褶痕相手蟹（S．plicata）线粒体16SrRNA基因部分片段的序列，二者的序列长度相同，均为533bp，且A、T、G、C的含量相似，分别为198bp（37．1％），206bp（38．6％），84bp（15．8％），45bp（8．4％）和200bp（37．5％），205bp（38．5％），81bp（15．2％），47bp（8．8％）；二者的序列有49处差异，其中21个位点为转换、22个位点为颠换和6个缺失／插入位点。进一步对20种相手蟹属蟹类的长度为361bp的16SrRNA基因同源序列进行分析，发现AT的含量为78．6％～82．9％，明显高于GC的含量，且存有91个变异位点。从NJ树和遗传距离来看，在分布于中国的3种相手蟹中，无齿相手型（S．dehaani）和红螯相手蟹的亲缘关系最近（d=0．0151），而它们与褶痕相手蟹的亲缘关系则较远（d=0．0924／0．09231。分布于中国的相手蟹和分布于北美的相手蟹之间存在着较大的遗传距离（差异），表明它们之间有着较远的亲缘关系，互为单系起源。