学科分类
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11 个结果
  • 简介:SulfatecanbeactivatedbyATPsulfurylaseandadenosine5’-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSK1genewasclonedanditsactivitywasanalyzed.OsAPSK1C36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSK1anditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3’-phosphoadenosine-5’-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.

  • 标签: RICE SULFATE ASSIMILATION ADENOSINE 5’-phosphosulfate KINASE
  • 简介:为在米饭的壳硅内容的QTLqHUS6以前位于米饭染色体6的短手臂。由使用在在个isogenic背景怀有qHUS6的RM587RM19784区域分离的张F2:3人口,为壳硅内容的二QTL被检测,哪个qHUS6-1在对着丝点近似的区域位于远侧的区域和qHUS6-2。在目标区域带小异质接合的片断的三米饭植物被选择,哪个二盖住qHUS6-1区域,其它盖住qHUS6-2区域。三张F2:3人口分别地从三植物的selfed种子被导出。印射的QTL用在qHUS6-1区域分离的二张人口被执行,并且qHUS6-1被标记RM510和RM19417限定到147.0-kb区域flanked。有在qHUS6-2区域的不同genotypic作文的五组F3线从另外的F2:3人口被选择。二QTL用双向ANOVA,qHUS6-2a位于RM19706RM19795和qHUS6-2b在间隔RM314RM19665定义的间隔被分开。

  • 标签: QTL检测 硅含量 染色体 水稻 QTL定位 自交种子
  • 简介:Moleculardesignbreedingisoneofstraightforwardapproachestobreakyieldbarriersinrice.Inthisstudy,GW6geneforgrainlengthandwidthfromBaodaliwastransferredintoanindicarecurrentparent9311andajaponicavarietyZhonghua11(ZH11)usingmarker-assistedbackcross(MAB).Oneandthreeintrogressionlineswereselectedforphenotypicanalysisfrom9311andZH11geneticbackgrounds,respectively.SSL-1,animproved9311nearisogeniclinewithGW6performed11%,19%and6.7%higherofgrainlength,1000-grainweightandsingleplantyield,respectively,ascomparedwith9311.AllthethreeimprovedZH11-GW6lines,R1,R2andR3,hadmorethan30%increaseingrainweightandabout7%higheringrainyield.SeedplumpnessofR1,R2andR3wasimprovedsynchronouslybecausethethreeZH11-GW6linescontainedGIF1(GrainIncompleteFilling1),adominantgrainfillinggene.Thus,GW6hashighpotentialinincreasingtheyieldofinbredlinesthroughMAB,makingitanimportantgeneticresourceinsuperhybridricebreeding.ThisstudyprovidesinsightsintheutilizationofGW6forlargegrainandhighyieldricebreedingviamoleculardesignbreeding.

  • 标签: 近等基因系 水稻产量 千粒重 分子改良 分子设计育种 人与生物圈计划
  • 简介:盆栽试验被进行学习不同的氮应用程序时间的效果(在期间直到ering或孕穗期)与样,氮在颖果开发和米饭变化Yangdao6的谷物质量评价。增加的氮肥(脲),特别在孕穗期期间适用,能显然增加milled,雪白大米率和蛋白质与控制(没有氮申请)相比在米饭满足谷物,并且减少白垩的谷物率和直链淀粉内容。而且,增加的氮肥显著地影响了颖果发展并且当氮适用在期间时,提高了粒重直到ering和孕穗期,特别在孕穗期期间。在颖果开发期间,增加的氮肥适用在期间直到ering和孕穗期能显然减少全部的淀粉和直链淀粉内容,然而并非显然为在米饭的胶淀粉内容谷物。氮肥的增加的顶肥,特别在孕穗期期间适用,在amyloplasts和proteinoplasts的发展和结构的有的重要效果。也就是说,它能改变分发,数并且amyloplasts和proteinoplastsin塑造特别在谷物腹部的内乳房间。与控制相比,amyloplasts和proteinoplasts的安排各是更靠近的,与更多的数字,更高的密度和更少的间隙星际ohter。而且,大多数amyloplasts在增加的氮肥水平下面显示出多面体。

  • 标签: 颖果 稻子 产品质量 直链淀粉
  • 简介:Aricepopulationconsistingof90TN1/GulyiguF3lineswasemployedtoanalyzethelinkagebetweenDNAmarkersandanewgeneWbph6(t)conferringresistancetowhitebackedplanthopper,Sogatellafurcifera.Byusingthemappingapproachofbulkedextremesandrecessiveclass,Wbph6(t)wasmappedontotheshortarmofchromosome11withageneticdistanceof21.2cMtoSSLPmarkerRM167.

  • 标签: 水稻 基因 限制性片段长度多态性 基因定位 单一序列长度多态性 抗性
  • 简介:与较普通活字大倍的杂交稻Yangliangyou6(YLY6)和Liangyoupeijiu(LYPJ),andthree线杂交稻Shanyou63(SY63)作为与谷物充满联合的材料,来源,水池和流动特征被调查。种子背景率,谷物充满度和YLY6和SY63的谷物产量比LYPJ的那些显著地高。在YLY6和SY63的灰煤杆和鞘的事的出口和转变百分比比LYPJ的那些显著地高。在谷物的蔗糖synthase,腺苷diphosphoglucosepyrophosphorylase,淀粉synthase和淀粉分叉酶的活动比为LYPJ为YLY6andSY63是更高的,并且很显著地与充满率,吝啬的谷物充满率,谷物充满度和粒重的最大的谷物被相关。每维管束的区域和YLY6和SY63的韧皮部的小穗状花小穗数字,谷物收益和全部的水池负担是比LYPJ的那些显著地小的,并且越大,越多并且越多降低种子背景率负担更差的谷物充满。交通率每YLY6的区域韧皮部比LYPJ或SY63的大。Theresults建议YLY6拥有强壮的来源,大水池活动和有效流动,它为它充满的高种子背景率和好谷物放了个生理的底。

  • 标签: 杂交技术 水稻 生物活性
  • 简介:Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

  • 标签: 水稻黑条矮缩病毒 相互作用 水稻基因 筛选 CDNA表达文库 非结构蛋白