简介:辣椒(CapsicumannuumL.)杂交种子纯度快速检测技术对于保证辣椒杂交品种种子生产具有重要的意义。红龙13号、红龙18号、红龙25号辣椒杂交种是新疆隆平红安生物科技有限责任公司选育并大面积栽培的主栽品种。为鉴定上述3个品种的纯度,本实验在特异性引物筛选的基础上,利用SSR标记技术对3个辣椒品种进行了种子纯度鉴定。结果表明,每个品种都有1对以上SSR引物可将其父本和母本分开,3个辣椒杂交种品种的纯度分别为95.35%、89.36%和95.25%。SSR分子标记方法可以准确鉴定辣椒杂交种纯度,且大大缩短纯度鉴定周期。
简介:将啤酒废酵母制成一种新型的生物吸附剂,用于吸附重金属离子铜。研究了不同温度、时间、pH值,以及铜离子浓度和酵母浓度条件下,啤酒废酵母对Cu^2+的吸附能力。初步确定了啤酒废酵母对Cu^2+吸附的最佳组合,即吸附温度为35℃,吸附时间为55min,起始pH值为5,酵母的质量浓度为1g/L,Cu^2+质量浓度为22mg/L,在此条件下啤酒废酵母对Cu^2+吸附率可达到90.00%左右。通过L16(4^5)正交实验表明,Cu^2+质量浓度和pH值是影响啤酒废酵母吸附铜离子能力的显著因素。对吸附了铜离子的啤酒酵母进行解吸实验,表明在浓度为1mol/L的HCl中,其解吸率为55.00%,与1mol/L的NaOH和去离子水相比,有较高的解吸率。
简介:ForestmanagementisexpectedtoinfluencesoilCO2efflux(FCO2)asaresultofchangesinmicroenvironmentalconditions,soilmicroclimate,androotdynamics.SoilFCO2ratewasmeasuredduringthegrowingseasonof2006inboththinningandnon-thinninglocationswithinstandsrangingfrom0to8yearsafterthemostrecentthinninginChinesefir(Cunninghamialanceolata(Lamb.)Hook)plantationsinHuitongEcosystemResearchStation,Hunan,China.SoiltemperatureandmoisturewerealsomeasuredtoexaminerelationshipsbetweenFCO2andsoilproperties.ForestthinningresultedinhugechangesinFCO2thatvariedwithtimesincecutting.Immediatelyfollowingharvest(year0)FCO2inthinningareaincreasedbyabout30%,declinedto20%–27%belowpre-cuttinglevelsduringyears4–6,andrecoveredtopre-cuttinglevelsat8yearspost-cutting.Asimilartemporalpattern,butwithsmallerchanges,wasfoundinnon-thinninglocations.TheinitialincreaseinFCO2couldbeattributedtoacombinationofrootdecay,soildisturbance,andincreasedsoiltemperatureingaps,whilethesubsequentdecreaseandrecoverytothedeathandgradualregrowthofactiveroots.StrongeffectsofsoiltemperatureandsoilwatercontentonFCO2werefound.ForestthinningmainlyinfluencedFCO2throughchangesintreerootrespiration,andthenetresultwasadecreaseinintegratedFCO2fluxthroughtheentirefellingcycle.