简介：目的：研究一株分离自条斑紫菜丝状体上的真菌代谢产物的活性成分。方法：利用电镜观察真菌形态特征,利用MEA培养基进行大规模发酵,经硅胶层析、葡聚糖凝胶层析等各种层析手段分离化合物,通过波谱技术鉴定化合物结构,并利用MTT法测定化合物抗肿瘤活性。结果：该菌属于曲霉属,分离得到了两个化合物1和2,分别为cyclo（L-Trp-L-Phe）（1）和WIN64821（2）。MTT法测试结果表明,cyclo（L-Trp-L-Phe）和WIN64821对37株人体肿瘤细胞株具有中等细胞毒活性,其平均IC50分别为3.3和5.8μg·mL^-1。结论：第一次报道这两种化合物的抗肿瘤活性。

简介：目的：对采自中国南海的海绵Axinyssasp．进行化学成分研究，从中寻找具有生物活性的次生代谢产物。方法：用硅胶和凝胶LH-20柱层析对海绵Axinyssasp．的乙醚提取物进行分离纯化，并结合现代波谱技术对得到的化合物进行结构鉴定。结果：分离得到了4个化合物，其结构分别鉴定为theonellinisothiocyanate（1），theonellinformamide（2），7-isothiocyanato-7，8-dihydro-α-bisabolene（3）和axinysterol（4）。结论：化合物1-3均为首次从海绵Axinyssasp．中分离得到，同时也是首次从中国海域的海洋生物中分离得到。

简介：Anewhexaketideacidesterifiedbythe17-hydroxylgroupof16,17-dihydroxycyclooctatin,namely17-[16,17-dihydroxycyclooctatinyl]-hexaketideester(1),amemberofthegroupofrarebacterialditerpeneswithafused5-8-5ringsystemwasisolatedfromstrainStreptomycessp.SR107.Thestructurewasdeterminedonthebasisofitsspectraldata(~1HNMR,~(13)CNMR,~1H-~1HCOSY,HSQC,HMBC,NOESY,IRandHR-ESI-MS).Theantibacterialactivitywasalsoevaluatedinthispaper.

简介：MarinesedimentsampleswerecollectedfromthecoastalareasofSouthernIndia,particularlyinKanyakumariDistrict.Twenty-eightdifferentfungalstrainswereisolated.Thescreeningoffungifrommarinesedimentwasdonetoisolateapotentfungusthatcanproducebioactivecompoundsforbiomedicalapplications.OnlythreestrainsvizTrichodermagamsiiSP4,TalaromycesflavusSP5andAspergillusoryzaeSP6werescreenedforfurtherstudies.Theintracellularbioactivecompoundswereextractedusingsolventextractionmethod.Thecrudeextractsweretestedforitsanti-microbialandanti-cancerpropertiesandanalyticallycharacterizedusingGasChromatographyMassSpectrometry(GC-MS).Allthethreeextractswereactive,buttheextractfromT.flavusSP5wasfoundtobemoreactiveagainstvarioushumanpathogens,viz.,PseudomonasaeruginosaATCC27853(17.8±0.1),EscherichiacoliATCC52922(18.3±0.3),andCandidatropicalisATCC750(17.7±0.4).ItalsoexhibitedcytotoxicactivityagainstHEp2carcinomacelllinewiththeLC_(50)valueof25.7μg·mL~(-1).TheGC-MSdatarevealedthepresenceofeffectivebioactivecompounds.TheseresultsrevealedthattheextractfromisolatedfungusT.flavusSP5actedasapotentantimicrobial,antifungal,andanticanceragent,providingbasicinformationonthepotencyofmarinefungitowardsbiomedicalapplications;furtherinvestigationmayleadtothedevelopmentofnovelanticancerdrugs.

简介：AIM:JS-38(mitothiolore),asyntheticversionofametaboliteisolatedfromXenorhabdussp.,wasevaluatedforitsanti-tumorandwhitebloodcell(WBC)elevatingactivities.METHOD:Theseanti-proliferativeactivitieswereassessedinvitrousingapaneloftencelllines.Theanti-tumoractivitiesweretestedinvivousingB16allograftmousemodelsandxenograftmodelsofA549humanlungcarcinomaandQGYhumanhepatomainnudemice.Theanti-tumorinteractionsofJS-38andcyclophosphamide(CTX)or5-fluorouracil(5-Fu)werestudiedinaS180sarcomamodelinICRmice.SpecificstimulatoryeffectsweredeterminedonperipheralneutrophilsinnormalandCTX-and5-Fu-inducedneutropenicmice.RESULTS:TheIC50valuesrangedfrom0.1to2.0μmol·L-1.JS-38(1μmol·L-1)causedanincreaseinA549tumorcellapoptosis.Multi-dailygavageofJS-38(15,30,and60mg?kg-1?d-1)inhibitedinvivotumorprogressionwithoutasignificanteffectonbodyweight.JS-38additivelyenhancedtheinvivoanti-tumoreffectsofCTXor5-Fu.JS-38increasedperipheralneutrophilcountsandneutrophilratesinnormalBALB/cmicealmostaseffectivelyasgranulocytecolony-stimulatingfactor(G-CSF).InmicewithneutropeniainducedbyCTXor5-Fu,JS-38rapidlyrestoredneutrophilcounts.CONCLUSION:TheseresultssuggestthatJS-38hasanti-tumoractivity,andalsohastheabilitytoincreaseperipheralbloodneutrophils.

简介：ThepresentstudyinvestigatedthechemicalcompositionofethylacetateextractsfromanendophyticactinomyceteStreptomycessp.A0916anditshostPolygonumcuspidatum.Acomparativeanalysisoftheantimicrobialandantioxidantpropertiesoftheextractswasalsoconducted.32compoundsofP.cuspidatumand23compoundsofStreptomycessp.A0916wereisolatedandidentifiedbyGC/MS.Antimicrobialactivitiesoftheextractswereevaluatedusingeightmicrobialstrains(3Gram-positivebacteria,3Gram-negativebacteria,and2fungi).TheStreptomycessp.A0916extractsshowedawiderangeofantimicrobialactivitiesandpresentedgreaterantimicrobialeffectivenessthantheP.cuspidatumextracts.Theminimuminhibitoryconcentration(MIC)ofStreptomycessp.A0916extractsagainsttheampicillin-resistantstrainEnterococcusfaeciumSIIA843was32μg·mL-1.Furthermore,theextractshadgreaterantimicrobialeffectagainstGram-positivebacteriathanGram-negativebacteria.Finally,theantioxidantactivityoftheStreptomycessp.A0916extractswasequaltothatoftheP.cuspidatumextracts.Inconclusion,ourresultssuggestthattheendophyticactinomycetesofthemedicinalplantsareanimportantsourceofbioactivesubstances.

简介：目的:研究NocardiaspNRRL-5646对三种不同母核的五环三萜皂苷元-齐墩果酸、熊果酸和23-羟基白桦酸的代谢.方法:在NocardiaspNRRL-5646的培养体系中加入底物后继续培养4天,发酵液经萃取后采用硅胶柱层析分离纯化,产物的结构根据光谱数据予以鉴定.结果:分离得到了三个代谢产物,分别是齐墩果酸甲酯、熊果酸甲酯、23-羟基白桦酸甲酯.结论:分离得到齐墩果酸甲酯、熊果酸甲酯、23-羟基白桦酸甲酯这三种微生物代谢产物,其中23-羟基白桦酸甲酯为首次分离得到的天然产物;首次发现NocardiaspNRRL-5646菌株特异性地将齐墩果酸、熊果酸、23-羟基白桦酸代谢为相应甲酯的代谢途径.