简介:BACKGROUND:GenetherapyforParkinson'sdiseaseisbeingexploredasaneffectivestrategytorestoreandprotectthefunctionofneuronalcellsinthesubstantianigra.Regulationofgeneexpressionisnecessaryforgenetherapytoavoidadverseeffectsduetoexcessivesynthesisoftransgeneproducts.OBJECTIVE:Herewedevelopedrecombinantadeno-associatedvirus(AAV)asaviralvector-mediatedgeneregulationsystembasedonCrerecombinasefusedtothemutatedligand-bindingdomainoftheestrogenreceptor(CreERT2)+inducingagenttamoxifen.InducibleCrerecombinasewasusedtoreducetyrosinehydroxylasegeneexpressionandtopreventtheexcessiveincreaseindopamine.DESIGN,TIMEANDSETTING:Ageneticengineeringinvitrocomparativestudyandrandomizedcontrolledanimalexperiment.ThisstudywasconductedattheGeneTherapyCenter,JichiMedicalSchool,JapanfromJune2002toJune2004.METHODS:ToconstructarecombinantAAVvectorcarryingadopaminesynthasegene.ThetyrosinehydroxylasegenewasinsertedusingaloxPfragmentthatcouldberegulatedbyCrerecombinase.TherecombinantAAVvectorcarryingtheCreERT2genewasco-transducedwithHEK293cellsandthecorpusstriatuminaratmodelofParkinson'sdisease,withinducingagenttamoxifentoregulategeneexpression.MAINOUTCOMEMEASURES:ThelevelsofdopamineandaromaticL-aminoaciddecarboxylase(AADC)activityweredetectedinHEK293cellmediumandinthecorpusstriatuminaratmodelofParkinson'sdiseaseusinghigh-performanceliquidchromatography.ImmunofluorescencedoublestainingwasusedtoobservetyrosinehydroxylaseandCreorAADCco-expressioninHEK293cellmedium.ImmunohistochemicalstainingwasemployedtoobservetyrosinehydroxylaseandAADCexpressionandbehavioralchangesweremeasuredinParkinson'srats.RESULTS:TransfectedAAV-CreERT2andAAVexpressingdopaminesynthesisenzymescouldincreasethesynthesisofdopamineinHEK293mediumandParkinson'sratstriatum(P<0.01)andimprovetherotationalbehaviorofParkin
简介:Anincreasingnumberofcomputersystemsarebeingviewedintermsofautonomousagents.Mostpeoplebelievethatagent-orientedapproachiswellsuitedtodesigningandbuildingcomplexsystems.Yet,todate,littleefforthadbeendevotedtodiscussingtheadvantagesofagent-orientedapproachasamainstreamsoftwareengineeringparadiam.Herebothofthisissuesandtherelationbetweenobject-orientedandagentorientedwillbeargued.Wedescribeanagent-orientedmethodologyandprovideaquotefordesigningaauctionsystem.
简介:INTRODUCTIONSincetheirintroductioninmid-1980s,polyamidoamide(PAMAM)dendrimershaveattractedconsiderableattentionbecauseoftheiruniquestructuresandproperties.Accordingtopreliminarystudiesinanimals,PAMAMdendrimersarenon-immunogenic,verylowinvivotoxicityandcanbeexcretedbyurineandfeces.
简介:Anincreasingnumberofcomputersystemsarebeingviewedintermsofautonomousagents.Mostpeoplebelievethatagent-orientedapproachiswellsuitedtodesignandbuildcomplexsystems.Yet.todate,littleefforthadbeendevotedtodiscusstheadvantagesofagent-orientedapproachasamainstreamsoftwareengineeringparadigm.Herebothofthisissuesandtherelationbetweenobject-orientedandagent-orientedwillbeargued.wedescribeanagent-orientedmethodologyandprovideaquotefordesigninganauctionsystem.
简介:Aprototypesystemforagent-baseddistributeddynamicservicesthatwillbeappliedtothedevelopmentofDataGridsforhigh-energyphysicsispresented.Theagent-basedsystemswearedesigninganddevelogpinggather,disseminateandcoordinateconfiguration,time-dependentstateandotherinformationintheGridsystemasawhole.Thesesystemsarebeingdevelopedasanenablingtechnologyforworkflow-managementandotherformsofend-to-endGridsystemmonitoringandmanagement.ThisprototypeisbeingdevelopedinJavaandisbasedontheJINI,MobileAgents,Self-OrganizingNeuralNetworks.
简介:Toenhancethesecurityofnetworksystems,putsforwardakindofsoftwareagentisputforward,whichhastheinductionabilityofnetworkframeworksandtheabilityofbehaviorindependence.Itismobilescanningagent.Moreattentionsispaidtoexpoundhowtodesignandrealizemobilescanningagent.Besides,itisalsoexplainedtheprogramsofmobilescanningagentsystem.Intheend,itexpectsmobilescanningagent.
简介:ThispaperintroducesasoftwareagentasavirtualplayerofthebusinessgamecalledBakeryGame.Thebusinessgameisatooltounderstandbusinessandmanagementprinciplesthroughexperienceinavirtualworld.Inordertoconstructsoftwareagentsforthegamingsimulation,whichisabletoparticipateinagameandbecomehumanplayers'worthyrival,wecombineasimplemodelthatconsidersthepropertyofBakeryGamewithastrategyadjustmentmodel.Theagenthasso-calledstrategyparametersthatareupdatedthroughtheexperience.Theagentchangeshisstrategydependingonparticularsituation.Theagentparticipatesinthegamethatisregeneratedfromlogdatainvarioussituations.
简介:AIMTo调查vasoactive的角色在形式剥夺的肠的肽(贵宾)近视(频分多路复用).METHODSFDM被放在三组八只小鸡创造一半透明在他们的右眼睛上更弥漫。Intravitreal注射盐并且贵宾一天被使用一次进组的堵塞眼睛2和3分别地。视网膜镜检法和轴的长度(AL)大小在第一和8th天更弥漫穿。在三个组的右眼睛和白天8上的第一个组的左眼睛的VIP受体和ZENK蛋白质的视网膜mRNA层次用在权利的中部的最后的折射(D)看的.RESULTSThe是的实时聚合酶链反应(PCR)被决定-13.75(-16.00,-12.00),-11.50(-12.50,-7.50),和-1.50(在组的-4.75,-0.75)1,2,和3,分别地(P<0.001)。在右眼睛的中部的AL(公里)是10.65(10.00,11.10),9.90(9.70,10.00),并且9.20(9.15,9.25)在组1,2,和3,分别地(P<0.001)。为VIP2受体的中部的三角洲三角洲周期阀值(CT)价值是1.07(0.82,1.43),1.22(0.98,1.65),0.29(0.22,0.45)在组的右眼睛1,2,和3,和1.18(0.90,1.37)在组1的左眼睛,分别地(P=0.001)。为ZENK蛋白质的中部的三角洲三角洲CT价值是1.07(0.63,5.03),3.55(2.20,5.55),在组的右眼睛无法发现1,2,和3和1.89(0.21,4.73)在组1的左眼睛,(P=0.001)分别地,.CONCLUSIONVIP在频分多路复用的发展有潜在的禁止的效果。
简介:Onthebasisofpreviouswork,thispaperdesignsanintelligentagentbasedonvirtualgeographicenvironment(VGE)systemthatischaracterizedbyhugedata,rapidcomputation,multi-user,multi-threadandintelligenceandissueschallengestotraditionalGISmodelsandalgorithms.Thenewadvancesinsoftwareandhardwaretechnologylayareliablebasisforsystemdesign,developmentandapplication.