简介：Microarraytechnologycanbeemployedtoquantitativelymeasuretheexpressionofthousandsofgenesinasingleexperiment.Ithasbecomeoneofthemaintoolsforglobalgeneexpressionanalysisinmolecularbiologyresearchinrecentyears.Thelargeamountofexpressiondatageneratedbythistechnologymakesthestudyofcertaincomplexbiologicalproblemspossible,andmachinelearningmethodsareexpectedtoplayacrucialroleintheanalysisprocess.Inthispaper,wepresentourresultsfromintegratingtheself-organizingmap(SOM)andthesupportvectormachine(SVM)fortheanalysisofthevariousfunctionsofzebrafishgenesbasedontheirexpression.Themostdistinctivecharacteristicofourzebrafishgeneexpressionisthatthenumberofsamplesofdifferentclassesisimbalanced.WediscusshowSOMcanbeusedasadata-filteringtooltoimprovetheclassificationperformanceoftheSVMonthisdataset.

简介：Directexposuretointensivevisiblelightcanleadtosolarretinopathy,includingmacularinjury.Thesignsandsymptomsincludecentralscotoma,metamorphopsia,anddecreasedvision.However,therehavebeenfewstudiesexaminingretinalinjuryduetointensivelightstimulationatthecellularlevel.Neuralnetworkarrangementsandgeneexpressionpatternsinzebrafishphotoreceptorsaresimilartothoseobservedinhumans,andphotoreceptorinjuryinzebrafishcaninducestemcell-basedcellularregeneration.Therefore,thezebrafishretinaisconsideredausefulmodelforstudyingphotoreceptorinjuryinhumans.Inthecurrentstudy,thecentralretinalphotoreceptorsofzebrafishwereselectivelyablatedbystimulationwithhigh-intensitylight.Retinalinjury,cellproliferationandregenerationofconesandrodswereassessedat1,3and7dayspostlesionwithimmunohistochemistryandinsituhybridization.Additionally,alight/darkboxtestwasusedtoassesszebrafishbehavior.Theresultsrevealedthatphotoreceptorswereregeneratedby7daysafterthelight-inducedinjury.However,theregeneratedcellsshowedadisruptedarrangementatthelesionsite.Duringtheinjury-regenerationprocess,thezebrafishexhibitedreducedlocomotorcapacity,weakenedphototaxisandincreasedmovementangularvelocity.Thesebehaviorsmatchedthemorphologicalchangesofretinalinjuryandregenerationinanumberofways.Thisstudydemonstratesthatthezebrafishretinahasarobustcapacityforregeneration.Visualimpairmentandstressresponsesfollowinghigh-intensitylightstimulationappeartocontributetothealterationofbehaviors.

简介：Grapheneanditsderivativeshaveattractedtremendousresearchinterestbecauseoftheiruniquecompositionandphysicochemicalproperties[1??3].However,thepotentialbiologicaltoxicityofGQDshasbecomeahealthriskbecauseoftheirinherentchemicalcompositionandnanoscaleproperties[4].Zebrafish(Daniorerio)isoneofthemostpromisinginvivomodelsystemsfortoxicitystudies[5].

简介：(GFI1)生长因素独立1为成年造血的干细胞(HSC)的哺乳动物的淋巴细胞和neutrophils和维护的成熟是重要的。在胚胎的造血作用的GFI1的角色是很好描绘的更少。通过一幅enhancer陷井屏幕和生物信息学分析，我们识别了Gfi1(命名gfi1.1)的一个斑马鱼相当或相同的事物并且在胚胎发育期间分析了它的功能。内长的gfi1.1基因和在它的genomic地点附近插入的GFP记者基因的表示在斑马鱼胚胎的造血的房间被检测。Morpholino(瞬间)gfi1.1击倒scl，lmo2，c-myb，mpo，rag1，gata1和血红素高山的减少的表示哈embryonic-1(hbae1)，以及胚胎的血红素的全部的数量，而是pu.1和l-plastin的增加的表示。在一样的条件下面，瞬间注射没影响涉及的标记脉管并且专业版肾的开发。相反地，在经由信使rna的gfi1.1的表示上，注射提高了gata1的表示，但是禁止了pu.1的表示。这些调查结果建议Gfi1.1在调整胚胎的erythroid和myeloid系决心的平衡起一个关键作用，并且也在斑马鱼期间为淋巴细胞和granulocytes的区别被要求胚胎开始。

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简介：Thevisualsystemisvitaltohumanhealth,andtheeffectsofionizingradiationonvisionhavereceivedincreasingattention.Usingazebrafishmodel,thisstudywasdesignedtoinvestigatetheapoptosisineyesinducedbycarbonionirradiation.Zebrafishembryosat12hpost-fertilization(hpf)wereirradiatedusing12C6+ionbeamsatdosesof2,4and8Gy.

简介：Inthisstudy,wedeterminedthecytotoxicityandgenotoxicityofcarbamateinsecticidecarbaryltofloundergill(FG)cellsanditsteratogenicitytozebrafishembryos.ThecytotoxicityofcarbaryltoFGcellswasdeterminedwithmethodsincludingMTTandneutralreduptaking(NRU),lactatedehydrogenase(LDH)releasingandHoechst33342andpropidiumidodide(PI)doublestaining.Moderatecytotoxicityinaconcentration-dependentmannerwasobserved.The24h-IC50valueof53.48±1.21,59.13±1.19and46.21±1.24mgL-1carbarylwasobtainedthroughMTT,NRUandLDHassays,respectively.DoublefluorescencestainingdemonstratedthatcarbarylinducedthedeathofFGcellsmainlythroughnecrosis.TherewasnosignificantgenotoxicityfoundintheFGcellsexposedtothehighesttestingconcentrationofcarbaryl(20mgL-1,P>0.05)aswasdemonstratedbyCometassay.Zebrafishembryosexposedtocarbarylatconcentrations≥10mgL-1displayedmoderatetoxiceffectsonthesurvival,spontaneousmovement,hatching,heartratesoftheembryosandtheirdevelopment,whichwereevidencedbyyolkandpericardialsacedemas,bodylengthreductionandtailflexureintime-andconcentration-dependentmannersatspecificstages.The24h-,48h-and96h-LC50valuesofcarbaryltozebrafishembryoswere41.80±1.10,17.80±1.04and14.46±1.05mgL-1,respectively.TheseresultssuggestedthatcarbarylismoderatelytoxictoFGcellsculturedinvitroandzebrafishembryos,andtheFGcellsweresimilartozebrafishembryosintheirsensitivitytocarbarylas24h-IC50andLC50indicated.

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简介：Understandingthehealtheffectsofexposuretospaceradiationisofparticularimportancebecauseofthemountinginterestindeepspaceexploration.Heavyionssuchas56Fearemainradiationsourcesindeepspace,whichcouldposeasignificanthazardtospaceflightcrewsduringandaftermissions[1].ComparedtoX-rays,56FeionshasthemostdeleteriouseffectsonsurvivalandlevelsofDNAdamage[2].

简介：Schisandrachinensis,atraditionalChinesemedicine(TCM),hasbeenusedtotreatsleepdisorders.Zebrafishsleep/wakebehavioralprofilingprovidesahigh-throughputplatformtoscreenchemicals,buthasneverbeenusedtostudyextractsandcomponentsfromTCM.Inthepresentstudy,theethanolextractofSchisandrachinensisanditstwomainlignincomponents,schisandrinandschisandrinB,werestudiedinzebrafish.Wefoundthattheethanolextracthadbidirectionalimprovementinrestandactivityinzebrafish.SchisandrinandschisandrinBwerebothsedativeandactivecomponents.WepredictedthatschisandrinwasrelatedtoserotoninpathwayandtheenthanolextractofSchisandrachinensiswasrelatedtoseoroninanddomapinepathwaysusingadatabaseofzebrafishbehaviors.ThesepredictionswereconfirmedinexperimentsusingCaenorhabditiselegans.Inconclusion,zebrafishbehaviorprofilingcouldbeusedasahigh-throughputplatformtoscreenneuroactiveeffectsandpredictmolecularpathwaysofextractsandcomponentsfromTCM.

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简介：Nano-sizedzincoxide(nZnO)particlesareonekindofthemostcommonlyusedmetaloxidenanoparticles(NPs).ThisstudycomparedthecytotoxicandembryotoxiceffectsofthreeincreasingsizedZnOparticles(30nm,80?150nmand2μm)inthefloundergill(FG)cellsandzebrafishembryos,andanalyzedthecontributionofsize,agglomerationandreleasedZn2+tothetoxiceffects.AllthetestedZnOparticleswerefoundtobehighlytoxictobothFGcellsandzebrafishembryos.Theyinducedgrowthinhibition,LDHrelease,morphologicalchangesandapoptosisinFGcellsinaconcentration-,size-andtime-dependentmanner.Moreover,thereleaseofLDHfromtheexposedFGcellsintothemediumoccurredbeforetheobservablemorphologicalchangeshappened.TheultrasonicationtreatmentandadditionofserumfavoredthedispersionofZnOparticlesandalleviatedtheagglomeration,thussignificantlyincreasedthecorrespondingcytotoxicity.ThereleasedZn2+ionsfromZnOparticlesintotheextracellularmediumonlypartiallycontributedtothecytotoxicity.AllthethreesizesofZnOparticlestestedinduceddevelopmentalmalformations,decreaseofhatchingratesandlethalityinzebrafishembryos,butsize-andconcentration-dependenttoxiceffectswerenotsoobviousasinFGcellspossiblyduetotheeasyaggregationofZnOparticlesinfreshwater.Inconclusion,bothFGcellsandzebrafishembryosaresensitivebioassaysystemsforsafetyassessmentofZnOparticlesandtheenvironmentalreleaseofZnOparticlesshouldbecloselymonitoredasfarasthesafetyofaquaticorganismsisconcerned.