简介:瞄准学习是评估LepidiumSativum悬崖的种子的使脱去脂肪的ethanolic摘录的抗氧化剂潜力的礼品的目的。在vitro化学药品试金viz不同的方法。DPPH(1,1-diphenyl-2-picrylhydrazyl)激进的清除,ABTS(2,清除的2-azinobis-(3-ethylbenzothiazoline-6-sulphonate)基,铁chelation,类脂化合物peroxidation,superoxide清除和非酶的血色素glycosylation试金被使用。摘录的全部的抗氧化剂能力被phosphomolybdic酸方法spectrophotometrically决定。使脱去脂肪的lepidium种子摘录给清除的重要自由激进分子看了的结果与结论在ABTS和非酶的glycosylation试金的活动,和在所有另外的试金的一项中等活动。在DPPH,ABTS,铁chelation,类脂化合物peroxidation和超级氧化物清除试金的摘录的IC50被发现是171.13,38.64,128.94,71.39和206.09g????????琭瑥慲票牤硯??敭桴硯??????è
简介:Thecompleteaminoacidsequenceofβ-momorcharin,aribosome-inactivatingproteinfromtheseedsofMomordicacharantiaLinn(Cucurbitaceae)hasbeendetermined.Thishasbeendonebythesequenceanalysisofpeptidesobtainedbyenzymaticdigestionwithtrypsin,chymotrypsinandS.aureusV8protease,aswellasbychemicalcleavagewithBNPS-skatole.Theproteinconsistsof249aminoacidresiduescontainingoneasparagine-linkedsugargroupattachedtothesiteofAsn51andhasacalculatedrelativemolecularmassof28,452Dawithoutadditionofthecarbohydrate.Comparisonofthissequencewiththoseoftrichosanthinandotherribosome-inactivatingproteinsfromdifferentspeciesofplantsshowsasignificanthomologywitheachother.Regardingthesimilarityoftheirbiologicalproperties,anactivedomainoftheseproteinshasbeenpredictedhere.