简介：AIM:Toevaluategutmicrobialdysbiosisintwovisceralhypersensitivemodelsincomparisonwithirritablebowelsyndrome(IBS)patientsandtoexploretheextenttowhichthesemodelscapturethedysbiosisofIBSpatients.METHODS:Visceralhypersensitivitywasdevelopedusingthematernalseparation(MS)ratmodelandpost-inflammatoryratmodel.Thevisceralsensitivityofthemodelgroupsandcontrolgroupwasevaluatedusingtheabdominalwithdrawreflexscoreandelectromyographyinresponsetogradedcolorectaldistention.The16SribosomalRNAgenefromfecalsampleswaspyrosequencedandanalyzed.Thecorrelationbetweendysbiosisinthemicrobiotaandvisceralhypersensitivitywascalculated.PositivefindingswerecomparedtosequencingdatafromapublishedhumanIBScohort.RESULTS:Dysbiosistriggeredbyneonatalmaternalseparationwaslastingbutnotstatic.BothMSandpostinflammatoryratfecalmicrobiotadeviatedfromthatofthecontrolratstoanextentthatwaslargerthantheco-housingeffect.Twoshortchainfattyacidproducinggenera,FusobacteriumandClostridiumXI,weresharedbythehumanIBScohortandbythematernalseparationratsandpost-inflammatoryrats,respectively,todifferentextents.FusobacteriumwassignificantlyincreasedintheMSgroup,anditsabundancepositivelycorrelatedwiththedegreeofvisceralhypersensitivity.Porphyromonadaceaewasaprotectivebiomarkerforboththeratcontrolgroupandhealthyhumancontrols.CONCLUSION:ThedysbiosisMSratmodelandthepost-inflammatoryratmodelcapturedsomeofthedysbiosisfeaturesofIBSpatients.Fusobacterium,ClostridiumXIandPorphyromonadaceaewereidentifiedastargetsforfuturemechanisticresearch.