简介:摘要:本文从安全生产实际需求出发,针对广州地铁二十一号线BAS专业获取AB PLC报文数据的需求,利用PYTHON基于EtherNet/IP实时获取PLC 标签或寄存器状态并保存本地,达到故障处理时协助定位的目的。
简介:摘要:本文从安全生产实际需求出发,针对广州地铁二十一号线BAS专业获取AB PLC报文数据的需求,利用PYTHON基于EtherNet/IP实时获取PLC 标签或寄存器状态并保存本地,达到故障处理时协助定位的目的。
简介:摘要 目的:探讨HIV-1核酸定量检测在HIV-1抗体确证试验不确定和阴性样本中的应用,为艾滋病及时诊断提供科学依据。方法:对204例HIV-1抗体确证试验不确定和阴性样本进行核酸定量检测,通过随访分别进行HIV-1抗体确证试验和核酸定量检测并结合流行病学资料确定其感染状况。结果:204例样本中,抗体不确定样本9I例,核酸定量检测≥5000CPs/ml 53例,低于检测线38例。抗体阴性样本II3例,核酸定量检测≥5000CPs/ml 2例,低于检测线111例。核酸定量检测≥5000CPs/ml 55中随访检测38例,抗体阳转38例占I00%,核酸定量检测≥5000CPs/ml 38例占100%,失访17例。核酸定量检测低于检测线的149例中,未见阳转病例,核酸定量检测低于检测线149例。结论:核酸检测作为补充试验的其中之一,在HIV-1抗体不确定和阴性样本中采用核酸定量检测有助于更快、更早对个体艾滋病感染者进行诊断。
简介:AbstractImportance:Bacteremia tuberculosis (TB) is a severe form of extrapulmonary TB. Studies assessing bacteremia TB in children are limited, especially for HIV-negative children.Objective:To explore the detailed clinical features of the bacteremia TB in children under 18 years of age.Methods:We reviewed the clinical records of the patients retrospectively and collected the strains isolated from their blood cultures. We used mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) to characterize the bacterial genotypes and alamarBlue to determine their drug susceptibility profiles. Polymerase chain reactions and DNA sequencing were used to identify drug-resistant mutations.Results:There were 13 pediatric bacteremia TB patients, 10 of whom were diagnosed with Bacillus Calmette–Guérin (BCG) bacteremia TB. Thirteen patients aged from 0.30 to 11.58 years were enrolled, of whom 76.92% were boys. All had fevers before hospitalization, and 76.92% had respiratory symptoms. All had received BCG vaccinations, and 46.15% had adverse post-vaccination reactions. Compared with Mycobacterium tuberculosis, BCG bacteremia was more likely to appear in younger children. Patients with BCG bacteremia had primary immunodeficiency diseases, and lower CD4, IgA, and IgE levels.Interpretation:Bacteremia TB was rapidly fatal in a large proportion of the immunodeficient children. Because classic findings may not be diagnostically specific, a high level of clinical suspicion is required, especially for patients with certain types of immunosuppression. Studies are needed to develop rapid diagnostic tests and to determine the value of empirical therapy in childhood bacteremia TB.
简介:摘要HIV-1储存库的持续存在是治愈HIV的主要障碍,在临床研究中,需要可靠的生物标志物对其进行标记。HIV-1 DNA在HIV-1储存库中可被持续检测到,在HIV-1感染诊断、预测病毒反弹和监测治疗效果等方面具有重要应用价值。PCR的检测技术是临床上常用的HIV-1 DNA检测方法,随着技术的不断创新与进步,可更准确地通过定性或定量检测感染细胞中总的、整合的和未整合的HIV-1 DNA。感染细胞中不同形式的HIV-1 DNA作为生物标志物在HIV感染监测和艾滋病治疗相关研究中报道日益增多。本文对感染细胞中HIV-1 DNA的检测方法及其作为生物标志物的临床应用进展进行综述。
简介:摘要HIV感染的窗口期是从HIV暴露到可持续量化检出病毒标志物的时期,抗体阳转的窗口期是从HIV暴露到稳定检出HIV抗体的时期。隐匿期(eclipse period)是从HIV暴露到血液中稳定检出病毒核酸的时期。理解窗口期是HIV检测咨询的基础,可以回答暴露后多长时间检测及重复检测、多长时间后检测的阴性结果可以排除HIV感染等关键问题,对于制定暴露后检测策略、选择检测方法和解释检测结果具有直接指导意义。本文介绍了窗口期的定义,强调了准确理解窗口期的要点,分析了影响窗口期的因素,特别是抗病毒药物对病毒标志物应答及检测的影响,提出了依据窗口期长短进行暴露后检测随访的方法和策略,以期为正确应用窗口期的概念指导暴露后检测暨急性期诊断提供参考。
简介:摘要:目的研究分析人类免疫缺陷病毒(HIV)/获得性免疫缺陷综合征(ADIS)并结核病20例患者的临床特征。方法:以HIV/ADIS并结核病患者40例为对象,本研究在2019年3月开始,在2020年3月结束。详细统计所有患者一般资料,分析临床特征。结果:HIV相关指标如下所示:CD4+水平是2-591个/uL,平均值是(231.12±12.45)个/uL;CD8+水平是24-837个/uL,平均值是(401.58±12.46)个/uL;CD4+/CD8+水平是0.03-1.99%,平均值是(1.01±0.23)%。实施抗酸染色涂片诊断,阳性检出7例(35.00%)。严重免疫抑制患者5例,中度6例,轻度8例。实施胸片X线诊断,CD4+水平为2-350患者,表现多样,且多见粟粒样渗出、肺中间质性病变、下叶间质性病变;CD4+水平为350-591患者,可见肺上叶阴影,不伴或者伴有空洞。实施组织病理学诊断,严重免疫抑制患者典型结核肉芽肿形成不良,其中2例患者无典型结核肉芽肿形成。结论HIV/ADIS并结核病患者不具有特异性,表现复杂,病情严重,具有较高的致残率、死亡率,多采用抗结核、激素、抗感染等综合治疗,改善生活质量。