简介:ThispapereonsidersanARIMA(p,d,q)medel.Amethodforestimatingtheorderdandtestingthehypothesis:d=d0isgiven.Theasymptoticpropertiesoftheestimatorhavebeendiscussed.
简介:应用DNA聚合酶链式反应(PCR)技术,对p16抑癌基因(CDKN2)进行体外定点突变,在p16cDNA中引入第48位密码子CCG(Pro)→CTG(Leu)和第74位密码子GAC(Asp)→AAC(Asn)突变,构建了p16-P48L和p16-D74N突变体。野生型和突变型p16cDNA克隆于pcDNA3构建pCMV-p16、pCMV-p16P48L和pCMV-p16D74N真核表达载体,导入纯合缺失p16基因的人肺癌细胞株H460,经RNA点杂交、RNA印迹和细胞免疫化学染色,检测到P16表达。通过比较表达野生型和突变型P16的H460细胞在~3H-TdR掺入及细胞所在周期的差异,证实P16表达抑制细胞进入S期,而P48L和D74N突变体对细胞进入S期没有什么影响,提示P48L和D74N突变导致P16蛋白功能丧失。
简介:ChinaandtheU.S.haveinitiatedaseriesofbilateralandmultilateralactionstodealwiththeNorthKoreannuclearcrisis.Inthelong-term,bilateralcooperationwilldependonPyongyang’sactions,U.S.attitudeonthenuclearissue,NorthKorea’spositioninChina’sgrandstrategyaswellasthebalancebetweenSino-U.S.strategiccompetitionandcooperation.
简介:PD(X3?)相互作用潜力用CCSD(T)理论和基础集合被构造,aug-cc-pV5Z。用这个潜力,分光镜的参数是精确地坚定的。礼品,De,Re,e,ee,e,并且具有3.05699eV,3.16175eV,0.14239nm,1701.558厘米?1,23.6583厘米?1,0.08599厘米?1,和4.3963厘米?1,分别地它几乎完美地符合大小。当时,26个震动的状态的一个总数被预言J=0由解决光线的Schr?原子运动的dinger方程。完全的震动的层次,古典拐弯处点,起始的旋转和离心的失真常数什么时候J=0第一次被报导,它有利地同意实验。全部、各种各样的部分波浪的生气的节在1.0魄为在二个地面状态P和D原子之间的有弹性的影响被计算吗??
简介:Inthispaper,wedeterminedanearthquakesequencelocationintheXingfengjiangareafromJune,2007toJuly,2014andthe3DP-wavevelocitystructurebyasimultaneousinversionmethod.Onthatbasis,westudiedtheoccurrencefeaturesofactivetectonicsandtheearthquakesourcemechanism.Theresultsshowthatthereservoirfracturesystemhasatendencytoincreasewithgradualdepthfromsoutheasttonorthwest,consistentwithgravitationalfieldresearchresults.Thereare4highvelocityzones(HVZ)underthedepthofthe7km-12kmcrustbetweentheXinfengjiangReservoirdamandXichangDistrict,Dongyuan.ThemaxvelocityofthebiggestHVZwhichisunderXichangis6.3km/s.Underthereservoirdamthereisastrongtectonicdeformationzone,asthecenterexitRenzishifault(F2),Nanshan-Aotoufaults(F4),Heyuanfault(F1)andShijiao-Xingang-Baitianfault(F5),7earthquakeswithML≥5.0(includingM6.1inMarch,1962)occurredatthehighgradientzoneoftheHVZⅢandHVZⅣedgewhichhasbeenunderthereservoirdamsince1960,withrelativityenergyreleasingmorethoroughly.ModerateseismicactivityoccurredattheHVZⅠedgewhichhasbeenunderXichangsince2012,andisadangerzoneforM5.0earthquakesinthefuture.