简介:Afterpre-cultureandtreatmentofosmosis,cotyledonsofimmaturepeanut(ArachishypogaeaL.)zygoticembryosweretransformedviaparticlebombardmentwithaplasmidcontainingachimerichphgeneconferringresistancetohygromycinandachimericintron-gusgene.Selectionforhygromycinresistantcallusesandsomaticembryoswasinitiatedat10thdpost-bombardmentonmediumcontaining10-25mg/Lhygromycin.Undercontinuousselection,hygromycinresistantplantletswereregeneratedfromsomaticembryosandwererecoveredfromnearly1.6%ofthebombardedcotyledons.ThepresenceandintegrationofforeignDNAinregeneratedhygromycinresistantplantswasconfirmedbyPCR(polymerasechainreaction)fortheintron-gusgeneandbySouthernhybridizationofthehphgene.GUSenzymeactivitywasdetectedinleafletsfromtransgenicplantsbutnotfromcontrol,non-transformedplants.Theproductionoftransgenicplantsaremainlybasedonanewlyimprovedsomaticembryogenesisregenerationsystemdevelopedbyus.
简介:BaseonthestrategicbombardmenttodestroywillpowerofChineseAnti-Japanesewar,intheSecondWorldWar,JapaneseaircraftswerenondiscriminatorilybombingChina’scities,whichcommittedcrimesagainsthumanity.Actually,theUSarmynondiscriminatorilybombedHiroshimaandNagasakianddamagedthehumanrightstoo.TheUSarmy,however,speededupJapanesesurrenderandprotectedmorepeople’srightstolife.ItisneverforgottenthatthepainfulgameofprotectinganddamaginghumanrightsbetweenJapanesemilitarismandanti-fascistalliance.
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简介:Thenegativeionfastatombombardment(NIFAB)massspectraofmono-,di-saccharidesandglycosidesusingphenylboronicacid(PBA)asreagenthavebeenstudied.Intheionsource,PBAreactsstereospecificallywiththemoleculescontainingcis-vicinalglycolstoformcharacteristicions,fromwhichthestereo-isomersofsaccharidescanbedefinitelydistinguished.Disaccharidesandglyco-sideswithβ-glycosidiclinkageseemtobeunfavorabaletoreactwithPBA,therefore,bycomparisonoftheabundancesofthecharacteristicions,theconfigurationoftheglycosidiclinkageinthesecompoundsmaybeinferred.