学科分类
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5 个结果
  • 简介:Afterpre-cultureandtreatmentofosmosis,cotyledonsofimmaturepeanut(ArachishypogaeaL.)zygoticembryosweretransformedviaparticlebombardmentwithaplasmidcontainingachimerichphgeneconferringresistancetohygromycinandachimericintron-gusgene.Selectionforhygromycinresistantcallusesandsomaticembryoswasinitiatedat10thdpost-bombardmentonmediumcontaining10-25mg/Lhygromycin.Undercontinuousselection,hygromycinresistantplantletswereregeneratedfromsomaticembryosandwererecoveredfromnearly1.6%ofthebombardedcotyledons.ThepresenceandintegrationofforeignDNAinregeneratedhygromycinresistantplantswasconfirmedbyPCR(polymerasechainreaction)fortheintron-gusgeneandbySouthernhybridizationofthehphgene.GUSenzymeactivitywasdetectedinleafletsfromtransgenicplantsbutnotfromcontrol,non-transformedplants.Theproductionoftransgenicplantsaremainlybasedonanewlyimprovedsomaticembryogenesisregenerationsystemdevelopedbyus.

  • 标签: 花生 体细胞胚 潮霉素抗性 粒子辐射
  • 简介:Excitation Function of Fission and Transfer Reaction in Bombardment of 40Ar on 159Tb NucleiExcitationFunctionofFissionan...

  • 标签: 40Ar FUNCTION
  • 简介:BaseonthestrategicbombardmenttodestroywillpowerofChineseAnti-Japanesewar,intheSecondWorldWar,JapaneseaircraftswerenondiscriminatorilybombingChina’scities,whichcommittedcrimesagainsthumanity.Actually,theUSarmynondiscriminatorilybombedHiroshimaandNagasakianddamagedthehumanrightstoo.TheUSarmy,however,speededupJapanesesurrenderandprotectedmorepeople’srightstolife.ItisneverforgottenthatthepainfulgameofprotectinganddamaginghumanrightsbetweenJapanesemilitarismandanti-fascistalliance.

  • 标签: Chinese ANTI-JAPANESE War nondiscriminatorily BOMBING right
  • 简介:Thenegativeionfastatombombardment(NIFAB)massspectraofmono-,di-saccharidesandglycosidesusingphenylboronicacid(PBA)asreagenthavebeenstudied.Intheionsource,PBAreactsstereospecificallywiththemoleculescontainingcis-vicinalglycolstoformcharacteristicions,fromwhichthestereo-isomersofsaccharidescanbedefinitelydistinguished.Disaccharidesandglyco-sideswithβ-glycosidiclinkageseemtobeunfavorabaletoreactwithPBA,therefore,bycomparisonoftheabundancesofthecharacteristicions,theconfigurationoftheglycosidiclinkageinthesecompoundsmaybeinferred.

  • 标签: REAGENT distinguished definitely linkage isomers STEREO