简介:Aredsoil,afluvo-aquicsoilandapermeablepaddysoilwereusedinalong-terminvestigationtostudychangesinnitrificationwithtreatments:1)soilincubation,2)liquidincubationinoculatedwithsoilsamples,and3)liquidincubationinoculatedwithammonia-oxidizingbacteria(AOB)fromthesoils.Thereweresignificantdifferences(P<0.001)innitrificationratesamongthethreesoilswhenmeasuredfor28daysbyadding(NH4)2SO4attherateof154mgNkg-1drysoiltofreshsoil.However,theamountsofnitrifyingbacteriainthethreesoilswerenotrelatedtosoilnitrificationcapacity.WhenthesoilsamplesortheisolatesofAOBenrichedfromthecorrespondingsoilwereincubatedinliquidwithpH5.8,7.0and8.0buffersand10mmolL-1ammoniumnitrogen,therewerenosignificantnitrificationdifferencesinthesamesoiltypeateachpH.TheabilitytooxidizeammoniathroughAOBfromdifferenttypesofsoilsinahomogeneousculturemediumwassimilar,andthesoilnitrificationcapacitycouldreflecttheinherentpropertiesofasoil.AlteringtheculturemediumpHofindividualsoiltypealsoshowedthatacidificationofanalkalinefluvo-aquicsoildecreasednitrificationcapacity,whereasalkalinizationoftheacidicredsoilandpermeablepaddysoilincreasedtheirnitrification.Forabetterinsightintofactorsinfluencingsoilnitrificationprocesses,soilpropertiesincludingtextureandclaycompositionshouldbeconsidered.
简介:ThegeneticdifferentiationofKoreanpine(Pinuskoraiensis)indifferentaltitudesinChangbaiMountainwasanalyzedbyISSRtechnique,anditwasfoundthatthelevelofgeneticdiversityofKoreanpinereducesalongwithaltitudeincreasinginChangbaiMountain.ThevariationofKoreanpineismainlyfromintra-populationandthereisapositiverelativitybetweengeneticdistanceandverticalgeographicdistanceofKoreanpineindifferentaltitudes.ThegeneticcoherenceshowsthataltitudehaslessinsulationtoKoreanpine.Therefore,itisdeducedthattheterrainformationofverticaldistributionofKoreanpineisaresultofdiffusionfromloweraltitudetohigheraltitudeinthecourseofenlargingitsadaptability.
简介:Basedonthestrongchelatingpropertyofbathophenanthrolinedisulfonicacid(BPDS)withFe(Ⅱ),rootFe(Ⅲ)chelatereductaseactivityisusuallymeasuredwithaspectrophotometerusingMES(2-morpholinoethanesulfonicacid)orHEPES(2-(4-(2-Hydroxyethyl)-1-piperazinyl)ethanesulfonicacid)bufferinthedarkbecauseofhighautoreductionrateofFe(Ⅲ)inthepresenceoflight.However,theexclusionoflightisinconvenient,especiallywhenanalyzingalargenumberofsamples.TheobjectiveofthisstudywastodevelopanewmethodfordeterminationofrootreductaseactivityundernormallaboratoryconditionsusingasuitablebuffercompositionandFe(Ⅲ)concentrationtoeliminatetheautoreductionofFe(Ⅲ).AmodifiedmethodusingaTris(2-amino-2-hydroxymethyl-1,3-propanediol)bufferatpH7.5insteadofMESorHEPESbufferandadecreasedFeEDTA(Feethylenediaminetetraaceticacid)concentrationof50μmolL-1wasdeveloped.TheautoreductionofFe(Ⅲ)usingtheTrisbufferwasundetectablefortemperaturesat4and28℃andwasalsomuchlowerthanthatusingtheotherbuffersevenwithsunlightduringmeasurementofFe(Ⅲ)reduction.Furthermore,thedifferencesinFe(Ⅲ)reductaseactivityamong5plantspeciesand14redclovercultivars(TrifoliumpratenseL.)couldbeeasilydetectedwiththemodifiedmethod.ThemethoddevelopedinthisstudytomeasurerootFechelatereductaseactivitywasnotonlyeffectiveandreliablebutalsoeasilymanagedundernormallaboratorylightconditions.
简介:Effectofdifferentshadinglevels(no-shading,80%shading,and40%shading)onphotosyntheticandstomatalresponsesincottonleaveswereinvestigatedunderconditionsofdifferentsoilwatercontentsinsummermidday.Allcottonleavesexhibitedsimilarbasicresponsestoshading,includingdecreasednetphotosyntheticrates,atendencytodecreaseintranspirationrates,andincreasedstomatalconductanceandintercellularCO2concentration.Theleafconductanceof80%shadedand40%shadedplantsincreasedby28%and16.7%comparedwithno-shadedplantsathighwater,respectively,butthenetphotosyntheticratesof80%shadedand40%shadedplantsdeclinedby50%and14.73%,respectively,Resultsshowedthatcombinedeffectofsoilmoistureandshadingonphotosyntheticandstomatalresponsesincottonleaveswasveryremarkable.
简介:Effectsofcolumntemperatureandflowrateonseparationoforganicacidswerestudiedbydeterminingninelow-molecular-weightorganicacidsonreversed-phaseC18columnusinghighperformaceliquidchromatography(HPLC)withawavelengthofUV(ultraviolet)214nmandamobilephaseof18mmolL^-1KH2PO4buffersolution(pH2.1).Thethermalstabiltiyoforganicacidswasdeterminedbycomparingtherecoveriesoforganicacidsindifferenttemperaturetreatments.Therelationshipsbetweencolumntemperature,flowrateorsolventpHandretentiontimewereanalyzed.AtlowsolventpH,separatioinefficiencyoforganicacidswasincreasedbyraisingtheflowrateofthesolventbecauseofloweringtheretentiontimeororganicacids.Highcolumntemperaturewasunfavorablefortheseparationoforganicacids.Theseparatingeffectcanbeenhancedthroughreducingcolumntemperatureinorganicaciddeterminationduetoincreasingretentiontime.Highthermalstabilityoforganicacidswithlowconcentrationswasobservedattemperatureof40℃-45℃,SensitivityandseparationeffectoforganicaciddeterminationbyHPLCwereclearlyimprovedbyacombinationofraisingflowrateandloweringcolumntemperatureatlowsolventpH.
简介:Methodsfordeterminingnienelowmolecular-weightoragnicacidsinrootexudatesweredevelopedbyusingreversed-phasehighperformanceliquiedchromatographywithUV(ultraviolet)detectionat214nm.Themobilephasewas18mmolL^-1kH2PO4adjustedtopH2.25withphosporicacidndtheflowratewas0.3mLmin^-1,Theanalyticalcolumnwasareversed-phasesilicabasedC-18column(shim-packCLC-ODS).Therootexudateswerecollectedthroughsubmergingthewholerootsystemintoaerateddeionizedwaterfor2hours,Thefilteredexudatesolutionswereconcentratedtodrynessbyrotaryevaporationat40℃,dissolvedin10mLmobilephase.Thechromatoraphicconditionsoforganicaciddeterminationwereanalyzed.Theresultsshowedthattherewasahighselectivityandsensitivityintheorganicaciddeterminationbyreversed-phasehighperformanceliquidchromatography.Coefficientsofvariationfororganicacieddeterminationwerelowerthan10%exceptlacticacid.Therecoverieswereconsistentlybetween80.1%to108.3%.Detectionlimitswereapproximately0.05to4.5mgL^-1fororganicacidsexceptsuccinicacidwiththedetectionlimitof7.0mgL^-1.Phosphorusdeficiencymaycontributetothereleaseoforganicacidsinsoybeanrootexudatesespeciallymalic,lacticandcitricacids.
简介:丰田2号(试验号浙9102)和丰田5号(试验号浙465),是由浙江省农科院蚕桑研究所20世纪90年代初育成的桑树人工多倍体新品种.据育种单位和浙江桐乡市田间栽培及室内生物测试,丰田2号等多倍体品种的产叶量和养蚕成绩名列前茅,成为当地蚕农竞相推广的首选良种.为将多倍体桑品种新技术成果尽快转化为经济效益,江油从1998年起,从浙江省农科院蚕桑研究所等科教单位引进30余个近年来育成的桑树三、四倍体新品种,近年多年多点的实用经济性状的选拔试验.第一批(1999~2003)8个供试新品种中,以丰田2号和丰田5号达到桑叶增产能力强,养蚕效益高的选拔目标.现简介如后.