简介:为建立高纯度bacillomycinD(杆菌霉素D)脂肽的可放大制备工艺,促进其在农用抗生素领域应用价值的开发,经分离纯化及分子结构鉴定,确定了海洋源解淀粉芽孢杆菌BacillusamyloliquefaciensTYg3-2所产活性化合物为bacillomycinD脂肽类抗生素;选择精制过的天然培养基(酵母提取物和蛋白胨),与组分明确的速效碳源、氮源(葡萄糖和硫酸铵)组成复合培养基,结合响应面优化调整培养基配方,建立了bacillomycinD发酵水平高达990mg/L的5L罐发酵工艺,其20.62mg/(L·h)的比生产速率远高于文献报道的10.23mg/(L·h);在高品质原料基础上,发酵液经菌液分离、酸化沉淀、甲醇浸提、乙醚沉淀、大孔树脂吸附及硅胶柱层析工艺,可高效制备纯度达94.72%的样品。针对所制备的高纯度bacillomycinD样品进行了抑菌活性初步研究,证实其可离体拮抗德氏霉菌、玉米弯孢霉、稻绿核菌和长柄链格孢,并通过盆栽试验证实了bacillomycinD能提升同源芽孢杆菌TYg3-2的防效,500mg/L的bacillomycinD与4×10~6CFU/mL的TYg3-2菌体混合施用,对黄瓜炭疽病的防效可达95.3%,优于单独施用bacillomycinD(78.4%)和菌体(0)的防治效果。研究初步探索了bacillomycinD在农用抗生素领域的开发价值,同时其高纯度脂肽可放大制备工艺的建立至关重要,不仅能在保全生产菌(其芽孢为生防菌剂原料)的同时从其发酵液中分离和纯化目标脂肽,以促成对农用抗生素价值的深入研究,也能够满足中国农药登记相关毒理学试验对样品的要求(纯度〉85%的克量级样品),其结果可为芽孢杆菌源脂肽类抗生素的研究与开发提供参考。
简介:ThispaperhighlightstheresearchanddevelopmentactivitiesbeingdoneonurbanforestryinthePhilippines.TherolethatR&Dplaysinurbanforestryislikewisediscussedintheintroduction.Recommendedtreespeciesforurbanplantingarepresentedincludingtheircharacteristicsanddistribution.Researchesrelatedtourbanforestrybeingconductedarelistedwithashortdescriptionofeachproject.Developmentactivitiesonurbanforestryarelikewisediscussed.Finally,recommendedR&DagendaandresearchableareasbasedonthePhilippineAgenda21andtheDepartmentofEnvironmentandNaturalResources(DENR)R&DFrameworkarepresented.
简介:到怀有基因Pi-d2从pCB6.3kb,pCB5.3kb和pZH01-2.72kb的三不同表示向量转变了的米饭强风抵抗的转基因的米饭线的米饭强风的抵抗被分析。有Pi-d2基因的九根先进产生的转基因的米饭线显示了各种各样的抵抗到39米饭强风紧张,并且最高疾病抵抗的频率到达了91.7%。有Pi-d2基因的四根早产生的同型结合的转基因的线展出了抵抗到58米饭强风紧张中的超过81.5%个,显示出宽光谱的抵抗的特征。当在文化媒介的粗略的毒素的集中增加了,米饭强风真菌的粗略的毒素选择的转基因的胚胎的calli证明从转基因的米饭植物的不成熟的胚胎的胼胝正式就职率减少了。当粗略的毒素的集中到达了40%时,从转基因的线的不成熟的胚胎的胼胝正式就职率是49.3%,并且受体控制的是5%。在正式就职下面的地里的转基因的大米线的颈强风的疾病发生是0%~50%,显示转基因的大米线的大米强风抵抗比受体控制的高得多。
简介:Background:Monitoringforesthealthandbiomassforchangesovertimeintheglobalenvironmentrequirestheprovisionofcontinuoussatelliteimages.However,opticalimagesoflandsurfacesaregenerallycontaminatedwhencloudsarepresentorrainoccurs.Methods:Toestimatetheactualreflectanceoflandsurfacesmaskedbycloudsandpotentialrain,3DsimulationsbytheRAPIDradiativetransfermodelwereproposedandconductedonaforestfarmdominatedbybirchandlarchinGenheCity,DaXing’AnLingMountaininInnerMongolia,China.Thecanopyheightmodel(CHM)fromlidardatawereusedtoextractindividualtreestructures(location,height,crownwidth).Fieldmeasurementsrelatedtreeheighttodiameterofbreastheight(DBH),lowestbranchheightandleafareaindex(LAI).SeriesofLandsatimageswereusedtoclassifytreespeciesandlandcover.MODISLAIproductswereusedtoestimatetheLAIofindividualtrees.CombiningalltheseinputvariablestodriveRAPID,high-resolutionopticalremotesensingimagesweresimulatedandvalidatedwithavailablesatelliteimages.Results:Evaluationsonspatialtexture,spectralvaluesanddirectionalreflectancewereconductedtoshowcomparableresults.Conclusions:Thestudyprovidesaproof-of-conceptapproachtolinklidarandMODISdataintheparameterizationofRAPIDmodelsforhightemporalandspatialresolutionsofimagereconstructioninforestdominatedareas.
简介:Aspontaneousmutation,tentativelynamedd63,wasderivedfromthetwin-seedlingprogeniesofricecrossedbydiploidSARIIIandMinghui63.Comparedwithwild-typeplants,thed63mutantshowedmultipleabnormalphenotypes,suchasdwarfism,moretillers,smallerflagleafandreducedseed-settingrateand1000-grainweight.Inthisstudy,twoF2populationsweredevelopedbycrossingbetweend63andNipponbare,d63and93-11.Geneticanalysisindicatedthatd63wascontrolledbyasinglerecessivegene,whichwaslocatedontheshortarmofchromosome8,withinthegeneticdistanceof0.40cMfromRM22195.Hence,D63mightbeanewgeneastherearenodwarfgenesreportedontheshortarmofchromosome8.
简介:Themodernizationandinformatizationmanagementofancientandfamoustrees’managementisanimportantstandardwithwhichthemunicipalpublicresourcemanagementlevelofaregionorcitycanbemeasured.Designanddevelopmentofancientandfamoustrees’three-dimensionalmanagementinformationsystemwasrealizedbasedonvirtualplantbyusingintegratedtechniquesofvirtualplantsandGeographicInformationSystem.Thesystemarchitecturedesignwasdevelopedonthebasisoffunctionalrequirements,andthepracticalsystemwasachievedinVisualStudio2008developmenttoolsandOpenGLgraphicsstandards.Thesystemhasfunctionofancienttreesarchivesmanagement,three-dimensionalreconstructionofthetreessurroundingenvironment,individualtreeinformationinquiryinthree-dimensionalscene,etc.Applicationanddisseminationofthissystemwillgreatlypromotethemanagementandprotectionofancientandfamoustreesstandardization,informalizationandvisualization