简介:AnargonatmosphericpressureplasmajetwasemployedtotreatL929murinefibroblastsculturedinvitro.Experimentalresultsshowedthat,comparedwiththecontrolcells,thetreatmentoffibroblastswith15sofplasmaledtoasignificantincreaseofcellviabilityandcollagensynthesis,whilethetreatmentof25splasmaresultedinaremarkabledecrease.Explorationofrelatedmechanismssuggestedthatcoldplasmacouldup-regulateCyclinD1geneexpressionanddown-regulatep27geneexpressionatalowdose,whileitcoulddown-regulateCyclinD1expressionandup-regulatep27expressionatahigherdose,thusalteringthecellcycleprogression,andthenaffectingcellviabilityandcollagensynthesisoffibroblasts.