简介:Anovelfluorescentprobe9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA)wassynthesizedforthedetectionofnitricoxide(NO)andcharacterizedbyIR,1H-NMRandEI-MSspectroscopy.Basedonaphotoelectrontransfermechanism,thefluorescenceintensitiesofDABPAwereinvestigatedwiththedifferentconcentrationsofNO.Undertheoptimalexperimentalconditions,thefluorescenceintensityofDABPAhadagoodlinearrelationship(R2=0.9977)withNOconcentrationintherangefrom1×10-7to1.5×10-6mol/Lwithadetectionlimitof1×10-8mol/L.ThecytotoxicityinducedbyDABPAwasevaluatedbytheMTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide)assayforbiologicalapplication.Furthermore,theprobeDABPAhadalsobeensuccessfullyappliedtoreal-timeimageNOproducedinPC12cellsinthepresenceofL-arginine.
简介:以La(NO3)3·nH2O、Pr(NO3)3·6H2O、Ni(NO3)3·6H2O为原料,丙氨酸为分散剂,采用低温燃烧法合成了(x=0.1~0.9)系列钙钛矿型复合氧化物。用TGDSC、XRD、TPR、SEM等表征手段对样品进行表征。结果表明,La0.5Pr0.5NiO3在650℃开始形成稳定的钙钛矿结构,焙烧800℃时,表面晶粒均匀;随着Pr的取代度增大,Pr离子未能完全进入LaNiO3晶格中A位,以氧化物的形式存在于钙钛矿晶体表面,同时La1-xPrxNiO3表面存在两种不同活性的氧物种,缺陷氧结构数量随着取代度的增大而增大。