学科分类
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6 个结果
  • 简介:TwelvespeciesofcrustaceanfromFujiancoastwereexaminedforlectinswithdifferentanimalerythrocytes.Serumextractsfromallof12speciesshowedagglutinatingactivityagainstatleasttwotypesoftheerythrocytesused,whichrevealedtheexistenceoflectinsinthesespecies.Therewerefivespecies(Penaeusjaponicus,Lophosquillacostata,Charybdisferiatus,Portunuspelagicus,Scyllaserrata)whoseserumscouldagglutinatealltheerythrocytestested.Thelowestserumproteinconcentrationrequiredtoproduceerythrocytesagglutinationvariedremarkably,rangingfrom0.7μg/mLto8080μg/mL.ThestrongestactivitywasshownintheagglutinationofrabbiterythrocytebyserumofPenaeusvanaminas.Inhibitionassaysperformedwithsevenmono-andbisaccharidesshowedthatagglutinationofquailerythrocytesbyserumsofthreespecies(Portunuspelagicus,ScyllaserrataandSesarmasp.)werenotinhibitedbyanysugars,whileotherswereinhibitedbyatleastthreetypesofsugars.TheassayoftemperatureinfluenceonagglutinatingactivityshowedthatonlyPenaeusjaponicusretainedactivitywhentheserumwasheatedto90℃,andotherserumslosttheiractivityat40℃,50℃,60℃,70℃,80℃for10minutes,respectively.

  • 标签: 外源凝集素 血凝素 螃蟹 对虾 福建海岸 红血球胶合
  • 简介:为了学习简历,活跃代谢物由导出海绵的未耕作的共生者生产了,海绵Gelliodesgracilis的共生者的一个metagenomicDNA图书馆被构造。DNA的平均尺寸在图书馆插入是20kb。这个图书馆用纸圆盘assaying为抗菌素活动被屏蔽。二克隆对小球菌tetragenus显示了抗菌剂活动。这二克隆的代谢物通过HPLC被分析。结果证明他们的代谢物与主人E的那些相当不同。包含向量pHZ132的关口iDH5α和主人。这研究可以介绍一条新途径给探索简历海绵共生者的活跃代谢物。

  • 标签: 海绵 共生体 生物活性 代谢物 生物化学
  • 简介:由在飞机紧张上下文的矩形的开的沟的颤动隔离的问题数字地用有限元素代码被学习,PLAXIS。土壤媒介被假定线性有弹性、各向同性、同类使遭到了到生产不变的颤动的垂直泛音负担。现在的模型被把它与以前出版的工作作比较验证。一条沟的关键几何特征,即,它从刺激的来源的深度,宽度,和距离,关于瑞利波长被使正常化。颤动的垂直、水平的部件的变细被学习因为对沟地点的各种各样的沟尺寸变化了从一对一个被动盒子活跃。结果在非维的形式被描绘,结论在垂直的attenuating和水平颤动部件关于几何参数的效果被得出。屏蔽效率被障碍的规范的深度首先管理。除了在一些特定的盒子中,宽度的效果有很少意义。简化回归模型被开发估计平均振幅减小因素。对垂直颤动盒子适用的模型被发现在对以前出版的结果的优秀同意。

  • 标签: 表面振动 稳态振动 设计 开沟 屏蔽 回归模型
  • 简介:表示顺序标注的Porphyrayezoensis(EST)的genomic资源被利用识别简单顺序重复(SSR),或微卫星。这个方法拿了为基因身份的建立或为高多型性的获得使用EST和微卫星的优点。当基因标记whenmicrosatellites被附在基因,微卫星能被使用。由生物信息学分析揭示了,1162从21954颗ESTscontained微卫星和聚合分析显示984这些EST掉进112contigs,当另外的178EST是单条时。290唯一的包含SSR基因的一个总数被识别。AACSSR是微卫星的最人口多的类型。在所有微卫星之中占优势的GC富有的microsatelliteswere。

  • 标签: 条斑紫菜 表达序列标签 微卫星标记 虚拟筛选
  • 简介:Whitespotsyndromevirus(WSSV)-resistantmolecularmarkerswerescreenedfromtheselectivelybrednewvariety‘HuanghaiNo.2’ofFenneropenaeuschinensisusingunlabeled-probehigh-resolutionmelting(HRM)technique.AftertheartificialinfectionwithWSSV,thefirst96deadshrimpsandthelast96survivingshrimpswerecollected,representingWSSV-susceptibleand-resistantpopulations,respectively.Thegenotypesatwell-developed39singlenucleotidepolymorphisms(SNPs)lociwereobtained.AsrevealedintheChi-squaretest,3SNPs,genotypeA/AofcontigC364-89AT,genotypeA/AofC2635-527CAandgenotypeC/TofcontigC12355-592CT,werepositivelycorrelatedwithdisease-resistancetraits.Other2SNPs,genotypeG/GofcontigC283-145AGandgenotypeC/CofcontigC12355-592CT,werenegativelycorrelated.Moreover,analysiswithBlastXprogramfordisease-resistantSNPsindicatedthat3contigs,Contig283,Contig364andContig12355,matchedtothefunctionalgenesofeffectorcaspaseofPenaeusmonodon,peptidetransporterfamily1-likeprotein,and40SribosomalproteinS2ofPercaflavescenswithhighsequencesimilarity.Theresultswillbehelpfultoprovidetheoreticalandtechnicalsupportsformolecularmarker-assistedselectivebreedingofF.chinensis.

  • 标签: Fenneropenaeu CHINENSIS WSSV SNP molecular MARKER
  • 简介:Theredoxcycleofironandmanganeseisamajorgeochemicaprocessattheboundarylayersoflakesediments.LakeAha,whichliesinthesuburbsofGuiyangCity,GuizhouProvince,China,isamedium-sizedartificialreservoirwithseasonallyanoxichypolimnion,Long-termsedimentaryaccumulationofironandmanganeseresultedintheirenrichmentintheuppersediments,Intheanoxicseason,Fe^2+andMn^2+,formedbydiologicaloxidation,woulddiffuseuptooverlyingwatersfromsediments.However,theconcentrationofoxidation,woulddiffuseuptooverlyingwatersfromsediments,However,theconcentrationofFe^2+increasedlateranddecreasedearlierthanthatofMn^2+.Generally,sulfatereductionoccurredat6cmbelowthesediment-waterinterface.Whereas,intheanoxicseason.thereductionreacheduppersediments,inhibitingthereleaseofFe^2+,TheFeconcentrationofanoxicwaterisquicklydecreasedfromhightolowasaresultofreductionofthesuplhursystem.

  • 标签: 贵州 贵阳市 氧化还原作用 沉积物