简介:Heatshockproteins(HSPs),suchasHSP70andHSP90,areasuiteofhighlyconservedproteinsproducedinallcellularorganismswhentheyareexposedtostresses.Inaquaticanimals,theyhavebeenprovedtoplayimportantrolesinresponsetoenvironmentalpollutantsandparticularlyinthenon-specificimmuneresponsestopathogeninfections.Inthepresentstudy,theexpressionprofilesofHSP70andHSP90genesinhemocytesandhepatopancreasfromtheridgetailwhiteprawnExopalaemoncarinicaudainfectedwithWSSVandVibrioanguillarumweredetectedusingreversetranscriptionpolymerasechainreaction(RT-PCR).AfterWSSVchallenge,theexpressionlevelofHSP70genetranscriptsinthehemocytesandhepatopancreasincreasedtopeaklevelat6hand48h,respectively.HSP90genetranscriptsinhemocytesandhepatopancreaswereup-regulatedsignificantlyat12hand6h,respectively.DuringV.anguillarumchallenge,themRNAcontentofHSP70geneinhemocytesandhepatopancreasincreasedsignificantlyat12hand6hpost-infection,respectively.TheexpressionlevelofHSP90genebothinhemocytesandhepatopancreaswereup-regulatedinthefirst3h.TheexpressionpatternsofHSP70andHSP90genesinhemocytesandhepatopancreasshowedtemporalandspatialdifferencesafterchallengedwithWSSVandV.anguillarum.TheresultssuggestedthatHSPsmightbeinvolvedinimmuneresponsestopathogenschallengeinE.carinicauda.
简介:Observationsofaquasi-90-dayoscillationinthemesosphereandlowerthermosphere(MLT)regionfromApril2011toDecember2014arepresentedinthisstudy.Thereisclearevidenceofaquasi-90-dayoscillationintemperaturesobtainedfromtheKunmingmeteorradar(25.6°N,103.8°E)andSoundingoftheAtmosphereusingBroadbandEmissionRadiometry(SABER),aswellasinwindobservedbytheKunmingmeteorradar.Thequasi-90-dayoscillationappearstobeaprominentfeatureinthetemperaturesandmeridionalwindtidesandpresentsquiteregularcyclesthatoccurapproximatelytwiceperyear.Theamplitudesandphasesofthequasi-90-dayoscillationintheSABERtemperatureshowafeaturesimilartothatofupward-propagateddiurnaltides,whichhaveaverticalwavelengthof~20kmabove70km.Intheloweratmosphere,asimilar90-dayvariabilityispresentedinthesurfacelatentheatfluxandcorrelateswiththetemperatureintheMLTregion.Similartothequasi-90-dayoscillationintemperature,a90-dayvariabilityofozone(O3)isalsopresentintheMLTregionandisconsideredtobedrivenbyasimilarvariabilityintheupwardly-propagateddiurnaltidesgeneratedintheloweratmosphere.Moreover,the90-dayvariabilityintheabsorptionofultraviolet(UV)radiationbydaytimeO3intheMLTregionisaninsitusourceofthequasi-90-dayoscillationintheMLTtemperature.
简介:摘要:针对大型箱船90A总段(机舱棚)的舾装周期问题,提出了一种优化方案。通过改进吊装工艺,将单轨吊梁的预埋点焊工艺调整为先拼接整体后吊装的方式,显著提高了装配效率。同时,通过优化排气管等部件的设计,实现了吊装前的包棉处理,提高了常规管系的完整性。这些改进措施有效缩短了舾装周期,提高了整体装配质量。
简介:摘要:目前建筑行业全面推进精细化施工管理,本文依托一个体育建筑项目工程的实际情况,阐述了对其进行调查研究、实地考察,并对所收集的资料进行整理的 QC小组实践活动,归纳出影响软土地基中 90米钻孔灌注桩成孔质量合格率的不利因素,并制定了相应的措施去解决这些问题。通过实际施工反馈可知上述办法行之有效,有效地提高了灌注桩成孔质量的合格率。
简介:使用NCEP/NCAR、英国气象局哈德莱中心(MetOfficeHadleyCenter)HadISST以及NOAA提供的再分析资料分析了海温、海冰及雪盖异常对20世纪90年代末我国冬季气温和东亚冬季风(EAWM)年代际跃变的外部强迫作用,同时也对比分析了20世纪90年代EAWM年代际跃变与20世纪80年代EAWM年代际跃变特征和成因的一些差异。结果表明:20世纪80年代中期EAWM的年代际变化特征主要表现为全国一致偏冷型,同时中国近海的海温也偏低;该年代际变化的主要原因来自大气内部动力过程,而海温和海冰的作用不显著。20世纪90年代末EAWM年代际变化的特征表现为东亚北方气温显著偏冷而南方偏暖的南北反相变化分布;EAWM在20世纪90年代末的年代际变化受北大西洋海温和热带太平洋海温的共同影响。北大西洋显著的异常暖海温,激发一个向下游传播的波列,使得西伯利亚高压加强,EAWM加强,从而导致我国北方气温下降;同时,秋冬季北极海冰异常偏少和秋季欧亚雪盖偏多对东亚冬季风的增强也有一定的作用。此外,热带西太平洋的暖海温异常会导致在海洋性大陆地区有异常的辐合和对流增强,引起大气环流的Gill型响应,对流西侧的异常气旋在孟加拉湾至我国西南地区出现南风异常,使得东亚南部地区温度偏高。因此,20世纪90年代末之后东亚温度呈现南暖北冷的分布特征。