简介:文章主要研究纳米金溶胶的细胞毒性。通过[3H]-TdR掺入法,研究粒径在15—20nm的纳米金溶胶,溶胶中含有的柠檬酸钠溶液以及溶胶中含有的柠檬酸钠与PVP混合溶液对正常细胞(人体表皮细胞、人体皮肤成纤维细胞)和癌细胞(HeLa细胞、K562细胞)活性的影响,实验结果表明,纳米金溶胶中含有的柠檬酸钠与PVP对这四种细胞的活性基本没有影响,可以在一定浓度范围内促进人体正常皮肤细胞的增殖,并具有剂量(以50μmol/L浓度为临界点)和时间依赖性;对于癌细胞则需高于一定浓度(50μmol/L)才有显著的抑制作用。
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简介:Layeredcathodematerialsofhigh-temperaturelithiumbatteries,LiNi1/3Mn1/3Co1/3O2aresynthesizedbyasol-gelmethodwithvariationinfinalsinteringtemperatureforboreholeapplications.Thestructure,morphologyandhigh-temperaturedischargeperformanceoftheseresultingproductsareinvestigatedbyX-RayDiffraction(XRD),scanningelectronmicroscopy(SEM),laserparticlesizeanalysis,galvanostaticandpulsedischarge.Theresultsofstructuralanalysisindicatethatthesamplesinteredat800℃hasthecharacteristicsofgoodcrystallinity,narrowsizedistributionandlargespecificsurfaceareaatthesametime.Thedischargeexperimentsalsoindicatethatthissamplehasthebestelectrochemicalproperties,withthemaximumdischargecapacitiesof314.57and434.14mAh·g-1at200and300℃respectivelyandtheminimumcellinternalresistancesatbothtemperatures.
简介:Anovelfluorescentprobe9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA)wassynthesizedforthedetectionofnitricoxide(NO)andcharacterizedbyIR,1H-NMRandEI-MSspectroscopy.Basedonaphotoelectrontransfermechanism,thefluorescenceintensitiesofDABPAwereinvestigatedwiththedifferentconcentrationsofNO.Undertheoptimalexperimentalconditions,thefluorescenceintensityofDABPAhadagoodlinearrelationship(R2=0.9977)withNOconcentrationintherangefrom1×10-7to1.5×10-6mol/Lwithadetectionlimitof1×10-8mol/L.ThecytotoxicityinducedbyDABPAwasevaluatedbytheMTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide)assayforbiologicalapplication.Furthermore,theprobeDABPAhadalsobeensuccessfullyappliedtoreal-timeimageNOproducedinPC12cellsinthepresenceofL-arginine.
简介:One-dimensional(1D)nanomaterialsandnanostructureshavereceivedmuchattentionduetotheirpotentialinterestforunderstandingfundamentalphysicalconceptsandforapplicationsinconstructingnanoscaleelectricandoptoelectronicdevices.Zincsulfide(ZnS)isanimportantsemiconductorcompoundofII-VIgroup,andthesynthesisof1DZnSnanomaterialsandnanostructureshasbeenofgrowinginterestowingtotheirpromisingapplicationinnanoscaleoptoelectronicdevices.Thispaperreviewstherecentprogresson1DZnSnanomaterialsandnanostructures,includingnanowires,nanowirearrays,nanorods,nanobeltsornanoribbons,nanocables,andhierarchicalnanostructuresetc.Thisarticlebeginswithasurveyofvariousmethodsthathavebeendevelopedforgenerating1Dnanomaterialsandnanostructures,andthenmainlyfocusesonstructures,synthesis,characterization,formationmechanismsandopticalpropertytuning,andluminescencemechanismsof1DZnSnanomaterialsandnanostructures.Finally,thisreviewconcludeswithpersonalviewstowardsfutureresearchon1DZnSnanomaterialsandnanostructures.
简介:ThetransportpropertiesintheLa2/3(Ca1-xSrx)1/3MnO3(x=0,1/3,2/3)filmspreparedusingtheRFmagnetronsputteringmethodwereinvestigated.TheeffectoftheCa,Srdouble-dopingattheApositionintheLa2/3A1/3MnO3onthestructureofthetargetsandtransportofthefilmshasbeenstudied.Withtheincreaseofx,thestructuresofthetargetstransformfromtherhombohedralphasetothecubicphase;themetal-insulatorphasetransitiontemperature(Tp)ofthefilmsincreases;andthecorrespondingpeakresistivitydecreases.Allthephenomenacanbequalitativelyexplainedbythelatticeeffect.
简介:用原子力显微镜(AFM)技术观察汉坦病毒的基本形貌,分别对用戊二醛固定的Vero—E6细胞和用汉坦病毒感染过的Vero—E6细胞进行成像,在原子级或纳米级水平上观测病毒感染后细胞表面超微结构的变化。将病毒直接滴加到云母片上自然风干后进行扫描,可以清晰地观察到病毒的结构大小;用0.5%~2.0%浓度的戊二醛固定细胞,通过成像发现,固定液浓度高时虽然成像质量较好,但对细胞的损伤较大,降低固定液浓度,细胞的形态接近于生理状态,成像质量良好;用不同稀释度的病毒感染细胞后,用合适的戊二醛浓度固定细胞进行观察,发现病毒感染前后细胞的形态结构发生了较大的变化,并且发现其形态的变化与病毒感染的浓度有显著的相关性。