简介:AnexperimentwasconductedtostudytheeffectsofdifferentPbconcentrationsonriceseedgerminationandseedlinggrowthinthreecultivars(Jiayu293,Jiayu948andXieyou963).ThemethodofqualitativetraitscombiningwithquantitativetraitsbyorthogonalpolynomialswasappliedtoanalyzericeseedvigorbyregressionmodelTheseedsfromdifferentricecultivarshaddifferentsensitivitytoPbpoison.InthePbconcentrationof100-700mg/L,Pbsignificantlydecreasedrootlengthandshootheight,andthedegreeoftherestraintwouldaggravatewiththeincreasingofPbconcentration.However,Pbdidnotreducerootdryweightandshootdryweight,whichindicatedthatdifferentphysiologicalcharacteristicshaddifferentsensitivereactionstoPb.Thepredictingequationsofregressionweredeveloped,whichcouldbeusedtopredicttheeffectsofdifferentPbconcentrationsonriceseedvigor.
简介:QTLmappingforsevenqualitytraitswasconductedbyusing254recombinantinbredlines(RIL)derivedfromajaponica-japonicaricecrossofXiushui79/CBao.Theseventraitsinvestigatedweregrainlength(GL),grainlengthtowidthratio(LWR),chalkgrainrate(CGR),chalkinessdegree(CD),gelatinizationtemperature(GT),amylosecontent(AC)andgelconsistency(GC)ofheadrice.ThreemappingmethodsemployedwerecompositeintervalmappinginQTLMapper2.0softwarebasedonmixedlinearmodel(MCIM),inclusivecompositeintervalmappinginQTLIciMapping3.0softwarebasedonstepwiseregressionlinearmodel(ICIM)andmultipleintervalmappingwithregressionforwardselectioninWindowsQTLCartographer2.5basedonmultipleregressionanalysis(MIMR).ResultsshowedthatfiveQTLswithadditiveeffect(A-QTLs)weredetectedbyallthethreemethodssimultaneously,twobytwomethodssimultaneously,and23byonlyonemethod.FiveA-QTLsweredetectedbyMCIM,ninebyICIMand28byMIMR.ThecontributionratesofsingleA-QTLrangedfrom0.89%to38.07%.AlltheQTLswithepistaticeffect(E-QTLs)detectedbyMIMRwerenotdetectedbytheothertwomethods.FourteenpairsofE-QTLsweredetectedbybothMCIMandICIM,and142pairsofE-QTLsweredetectedbyonlyonemethod.Twenty-fivepairsofE-QTLsweredetectedbyMCIM,141pairsbyICIMandfourpairsbyMIMR.ThecontributionratesofsinglepairofE-QTLwerefrom2.60%to23.78%.IntheXiu-BaoRILpopulation,epistaticeffectplayedamajorroleinthevariationofGLandCD,andadditiveeffectwasthedominantinthevariationofLWR,whilebothepistaticeffectandadditiveeffecthadequalimportanceinthevariationofCGR,AC,GTandGC.QTLsdetectedbytwoormoremethodssimultaneouslywerehighlyreliable,andcouldbeappliedtoimprovethequalitytraitsinjaponicahybridrice.
简介:早成熟的indica米饭变化Zhefu49,质量和淀粉组织其谷物,对环境温度敏感,在在植物生长房间充满舞台的谷物受到不同温度(为高温度的32°C和为最佳温度的22°C),并且编码淀粉分叉的三isoform基因(SBEI,SBEIII和SBEIV)的不同表情在内乳的酶(SBE)被即时荧光学习量的PCR(FQ-PCR)方法。在开发米饭内乳的SBE表示上的高温度的效果是isoform依赖的。高温度显著地下面调整SBEI和SBEIII的表情,当时起来调整SBEIV的表示。与SBEIV和SBEIII相比,在Zhefu49米饭内乳的SBEI基因的表示对在充满舞台的谷物的温度变化更敏感。这研究显示那特别处于天气/气候条件变化由isoform表示证实了的温度压力影响米饭谷物形成和它的质量。