简介：布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之一在米饭正在引起hopper灼伤，并且从而减少生产率并且另外产品的质量。控制这个害虫的有效管理策略是到本地米饭栽培变种的理想的基因的鉴定和转移。为开发抵抗栽培变种的最重要的途径是标记的鉴定，它能在更持久的抵抗遗传型的帮助标记的选择帮助。易受影响的父母IR50和抵抗父母Ptb33，和他们的F2人口是为有随机的放大多态的DNA的抵抗基因的鉴定的使用的inbulkedsegregant分析标记(RAPD)教材。教材OPC7和OPAG14证明主导、易受影响的特定的banding模式那么叫了co主导的标记。而且，OPC7697和OPAG14680给抵抗特定的乐队看了并且因此在联合分阶段执行，而OPC7846和OPAG14650给易受影响的特定的genotypic乐队看了inbulkedsegregant分析。因此，联合阶段标记，OPC7697和OPAG14680，被认为在在庄稼改进的米饭遗传型的帮助标记的选择更有用。

简介：Nitrogen(N)useefficiencyisusuallylessthan50%,anditremainsamajorprobleminricecultivation.Controlledreleasefertilizer(CRF)technologyisoneofthewell-knowneffortstoovercomethisproblem.TheefficiencyofCRF,however,isverymuchdependentonthetimingofnutrientrelease.ThisstudywasconductedtodeterminetheprecisetimeofNuptakebyriceasaguidelinetodevelopefficientCRF.FertilizerNuptakebyriceatdifferentgrowthstageswasinvestigatedbyusing15Nisotopictechnique.Ricewasplantedinpots,with15NureaasNsourceattherateof120kg/hm2.Potassiumandphosphoruswereappliedatthesamerateof50kg/hm2.Standardagronomicpracticeswereemployedthroughoutthegrowingperiods.Riceplantswereharvestedeverytwoweeksuntilmaturationatthe14thweekandanalyzedfortotalNand15Ncontent.Nitrogenderivedfromfertilizerwascalculated.TotalNuptakeinplantsconsistentlyincreaseduntilthe11thweek.Afterthat,itstartedtoplateauandfinallydeclined.Moreover,Nutilizationbyriceplantspeakedat50%,whichoccurredduringthe11thweekaftertransplanting.Nderivedfromfertilizerinriceplantswereintherangeof18.7%to40.0%inallplanttissues.TheremainingNwasderivedfromsoil.Basedonthisstudy,NreleasefromCRFshouldcompletebythe11thweekafterplantingtoensurethemaximumfertilizerNuptakebyriceplants.EfficientCRFshouldcontributetohigherNderivedfromfertilizerwhichalsoresultedinahighertotalNuptakebyriceplants,increasingthepotentialofricetoproducehigheryieldwhileatthesametimeofreducingloss.

简介：AsoilpotcultureexperimentwithfoursuppliedPlevels(i.e.P30,P50,P100,P200,representingsupplementalP30,50,100,200mg/kg,respectively)wasconductedtoinvestigateuptakeanduseabilitytoPandZninthericegenotypeswithdifferentP-efficiency,ofwhichricegenotypes508,99011,580,99112werelow-Ptolerantand99056,99012werelow-Psensitive.Low-Ptolerantrice580and99011absorbedmorePthantheothers,andricegenotype580hadstrongeruptakeabilityespeciallyatlow-PlevelsuchasP50andP30.508couldabsorbconsiderableP,andhadthelowestPpercentageofshoot,indicatingithadgoodperformanceinP-useefficiency.ThesethreericegenotypeshadlargerbiomassandlessresponsetochangedPlevelthanricegenotype99112,99056and99012.Ricegenotype99112showedLow-Ptolerancemainlybysacrificingbiomasstomaintainhighrelativegrainyield.TheleastamountofPabsorbedby99056showedithadthelowestPuptakeefficiency,andthehighestPpercentageinshootof99012meantithadthelowestPuseefficiency.Sotheytwoshowedlow-Psensitivity.ZncontentsinshootunderP200,P100andP50weresimilar,butP30increasedZncontentinshootsignificantly.TheZncontentsinshootof99112,99056and99012werehigherthanthoseof508,99011and580,especiallyattilleringstageandbootingstage.AsfortotalZncontentinshoot,Low-Ptolerantricegenotype580hadthelargestamountandfollowedby99011and508,low-Ptolerantricegenotype99012hadthesmallestamountatthethreesamplingstageandfollowedby99056.Furthermore,P/Zninshootof99012wasthehighest,andthatof99056wasthesmallestatthesamePlevel.

简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

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