简介:AIM:Toevaluatetheefficacyandsafetyofcornealcollagencrosslinking(CXL)topreventtheprogressionofpost-laserinsitukeratomileusis(LASIK)cornealectasia.·METHODS:Inaprospective,nonrandomized,single-centrestudy,CXLwasperformedin20eyesof11patientswhohadLASIKformyopicastigmatismandsubsequentlydevelopedkeratectasia.Theprocedureincludedinstillationof0.1%riboflavin-20%dextranesolution30minutesbeforeUVAirradiationandevery5minutesforanadditional30minutesduringirradiation.Theeyeswereevaluatedpreoperativelyandat1-,3-,6-,and12-monthintervals.Thecompleteophthalmologicexaminationcompriseduncorrectedvisualacuity,bestspectacle-correctedvisualacuity,endothelialcellcount,ultrasoundpachymetry,cornealtopography,andinvivoconfocalmicroscopy.·RESULTS:CXLappearedtostabiliseorpartiallyreversetheprogressionofpost-LASIKcornealectasiawithoutapparentcomplicationinourcohort.UCVAandBCVAimprovementswerestatisticallysignificant(P<0.05)beyond12monthsaftersurgery(improvementof0.07and0.13logMARat1year,respectively).Meanbaselineflattestmeridiankeratometryandmeansteepestmeridiankeratometryreduction(improvementof2.00and1.50diopters(D),respectively)werestatisticallysignificant(P<0.05)at12monthspostoperatively.At1yearafterCXL,meanendothelialcellcountdidnotdeteriorate.Meanthinnestcorneapachymetryincreasedsignificantly.·CONCLUSION:Theresultsofthestudyshowedalong-termstabilityofpost-LASIKcornealectasiaaftercrosslinkingwithoutrelevantsideeffects.Itseemstobeasafeandpromisingproceduretostoptheprogressionofpost-LASIKkeratectasia,therebyavoidingordelayingkeratoplasty.
简介:·Glaucomaisoneoftheleadingcausesofvisualimpairmentandblindnessworldwide.Ofknownriskfactorsforglaucoma,anincreasedinintraocularpressureismosthighlycorrelatedwithglaucomatousdamage.Irrespectiveofthecause,apoptosisoftheretinalganglioncellsistheeventualoutcome.Itiswidelyacceptedthatglaucomaisaneurodegenerativediseasethatisstronglycorrelatedwithcentralnervoussystemdisorders,suchasAlzheimer’sdisease.Thesetwodisordersalsosharesomesimilaritiesinpathogenicmechanisms.Recentstudiessuggestthatthetransientreceptorpotentialcanonical6channelcouldworktogetherwithbrain-derivedneurotrophicfactortopromoteneuronsurvivalinbrainandretina.Inthisstudy,weproposethattransientreceptorpotentialcanonical6maycontributetothepathogenesisofhumanglaucomaandbecomeapotentialtherapeutictarget.
简介:准分子激光角膜切削术是九十年代在我国兴起的角膜屈光手术。近年来有诸多成功报道。但是,对于PBK术后的远期疗效乃是医生和患者极为关注的问题。我院自1996年4月开始用PRK治疗近视,效果较好,现对PRK术后5年以上患者进行随诊观察345例671眼,结果报告如下。资料与方法
简介:目的:研究柚皮素对激光诱发大鼠脉络膜新生血管形成(CNV)、高眼压兔眼血流和缺血大鼠眼视网膜功能恢复的作用。方法:选择雄性棕色挪威大鼠,采用激光诱发Bruch膜破裂后,予10g/L(20mg/kg)柚皮素,1次/d,持续4wk;光凝后2,4wk分别做眼底荧光血管造影,评估CNV的形成。采用彩色微球技术、眼电生理技术检测兔眼血流和大鼠视网膜功能恢复。结果:与对照组比较,10g/L柚皮素能明显增加高眼压兔眼脉络膜血流(P〈0.05),能明显增加缺血大鼠眼视网膜功能恢复(P〈0.05),能明显减轻光凝点荧光素渗漏(75.8%~95.0%,P〈0.01)。结论:柚皮素能抑制激光诱发大鼠络膜新生血管形成;增加高眼压兔眼脉络膜血流;增加缺血大鼠眼视网膜功能恢复。
简介:目的研究高眼压及其持续时间对大鼠视网膜caspase-3表达的影响。方法通过前房灌注平衡盐液建立大鼠急性高眼压模型:高眼压持续时间均为4h,依据眼内压的不同将sD大鼠60只随机分为正常对照组、40mmHg(1mmHg=0.133kPa)组、60mmHg组、80mmHg组、100mmHg组,每组12只。将眼内压为80mmHg的48只sD大鼠随机分为正常对照组、2h组、4h组、8h组,每组12只。正常对照组不给予前房灌注,其他各组分别给予不同的高眼压或高眼压持续不同时间。各组灌注结束后快速摘除眼球,分别采用Western印迹法和免疫组织化学法检测视网膜caspase-3的表达。结果与对照组相比,40mmHg组大鼠视网膜caspase-3的表达无增加(P〉0.05);60mmHg组、80mmHg组和100mmHg组大鼠视网膜caspase-3的表达均强于正常对照组(q值分别为4.87、5.28和6.71;P〈0.01),但各组之间差异无统计学意义(P〉0.05)。与对照组相比,2h组大鼠视网膜caspase-3的表达差异无统计学意义(P〉0.05),4h组和8h组大鼠视网膜caspase-3的表达均增加(q值分别为2.81和3.67;P〈0.01)。表达caspase-3的视网膜细胞主要位于神经节细胞层、外丛状层和内丛状层。结论采用前房灌注平衡盐液制作急性高眼压模型研究高眼压大鼠视网膜caspase-3的表达时,眼内压为80mmHg、高眼压持续4h即可。