简介:瞄准:为了检验因素,用一个最近确定的在试管内模型在肠的巨噬细胞(IMAC)的区别包含了。方法:测试是否可溶或膜界限因素induceIMAC区别,刚,elutriated单核白血球(瞬间)与肠的上皮细胞(IEC)的调节媒介或细胞膜被孵化或与在transwell系统的IEC有教养。决定瞬间的活跃迁居的重要性,从瞬间和IEC的1:1混合的三维的总数被免疫组织化学和流动血细胞计数检验。Apoptosis被caspase-3检验西方的污点。在区别模型的细胞外的矩阵生产被免疫组织化学比较。结果:IMAC区别在一个复杂三维的合作文化模型被观察(多细胞的球状体,MCS)与在进球状体的瞬间的移植以后的IEC。由有调节媒介或IEC的膜准备的瞬间的合作文化,没有IMAC区别被导致。有在transwell文化的IEC的瞬间的合作文化,与二个房间,膜也分开的人口没导致瞬间的象肠一样区别。与有在IEC和瞬间的混合MCS入境瞬间的IEC球状体相对照,瞬间的一张仅仅小潜水艇人口能熬过七个天文化时期。结论:瞬间在试管内的象肠一样区别仅仅在三维的MCS在在IMAC的区别期间显示房间矩阵或房间房间相互作用的一个角色的瞬间的移民以后建模的建筑群被导致。
简介:瞄准:讨论CT的临床的价值三维(3-D)在诊断胃肠道疾病的成像。方法:52个病人的Three-D成像调查结果回顾地被分析。Three-D成像方法包括的遮的表面显示(SSD),显示的体积(VR),虚拟内视镜检查法(VE)(MPR)并且多平面的重新格式化。CT3-D的诊断结果被比较内视镜检查法或外科的发现与那些评估。结果:有胃肠道疾病的52个病人被CT3-D成像,50个盒子正确地被诊断诊断,2被误诊。有胃的疾病的33个盒子(27与癌,5与消化性溃疡并且1与平滑肌瘤)并且19大肠的疾病(10与结肠癌,2与直肠的癌,5与结肠息肉并且2与回盲肠连接的肺结核)。有突出的损害的22个盒子(9与随后的空损害),20与洞的狭窄(8与伴随物突出的损害)并且10与空损害(5与伴随物突出的损害)在3-D图象被显示出。显示出的最小的损害是1.0厘米x0.8厘米x0.5厘米。结论:CT3-D成像,没有疼痛的非侵略的考试,能清楚地并且直接与精确地点和高诊断精确性显示胃肠道的损害。它是到内视镜检查法的一种重要互补技术。
简介:AIMTodescribeathree-dimensionalmodel(3DM)toaccuratelyreconstructanatomicrelationshipsofcentrallylocatedhepatocellularcarcinomas(HCCs).METHODS:FromMarch2013toJuly2014,reconstructionsandvisualsimulationsofcentrallylocatedHCCswereperformedin39patientsusinga3Dsubject-basedcomputedtomography(CT)modelwithcustomdevelopedsoftware.CTimageswereusedforthe3DreconstructionofCouinaud'spediclesandhepaticveins,andthecalculationofcorrespondingtumorterritoriesandhepaticsegmentswasperformedusingYorktalDMITsoftware.Therespectivevolume,surgicalmargin,andsimulatedvirtualresectionoftumorswerealsoestimatedbythismodelpreoperatively.Allpatientsweretreatedsurgicallyandtheresultswereretrospectivelyassessed.Clinicalcharacteristics,imagingdata,procedurevariables,pathologicfeatures,andpostoperativedatawererecordedandcomparedtodeterminethereliabilityofthemodel.RESULTS:3Dreconstructionallowedstereoscopicidentificationofthespatialrelationshipsbetweenphysiologicandpathologicstructures,andofferedquantifiableliverresectionproposalsbasedonindividualizedliveranatomy.Thepredictedvalueswereconsistentwiththeactualvaluesfortumormassvolume(82.4±109.1mLvs84.1±108.9mL,P=0.910),surgicalmargin(10.1±6.2mmvs9.1±5.9mm,P=0.488),andmaximumtumordiameterthenumberandextentofportalvenousramifications,aswellastheirrelationtohepaticveins,werevisualized.Preoperativeplanningbasedonsimulatedresectionfacilitatedcompleteresectionoflargetumorslocatedintheconfluenceofmajorvessels.Andmostofthepredicteddatawerecorrelatedwithintraoperativefindings.CONCLUSION:This3DMprovidesquantitativemorphometryoftumormassesandastereo-relationshipwithadjacentstructures,thusprovidingapromisingtechniqueforthemanagementofcentrallylocatedHCCs.