简介：目的:制备针对呼吸道合胞病毒(RSV) N蛋白的兔多克隆抗体，以其作为检测抗体建立基于ELISA的快速中和抗体检测方法。方法:构建pET30a-N质粒，表达纯化N蛋白，免疫新西兰兔制备针对RSV N蛋白的多克隆抗体作为检测抗体。阳性血清系列稀释后与100半数组织培养感染剂量(TCID 50)/孔的RSV中和，接种Hep-2细胞培养，80%丙酮固定细胞，ELISA方法检测感染细胞中病毒的N蛋白，当每孔的吸光度值低于临界值时，视为中和试验阳性孔，阳性孔血清的最高稀释度为该血清的中和抗体滴度。优化抗体稀释度、检测时间、细胞密度及中和时间，建立基于N-ELISA的中和抗体检测方法，对建立的实验方法进行细胞代次、边缘孔效应、准确性、重复性以及精密度验证，初步应用于人RSV IgG阳性血清检测，分析与微量中和法的相关性。 结果:成功构建出pET30a-N质粒，表达纯化

简介：【摘要】：宫颈癌是女性生殖系统最常见的恶性肿瘤，严重危害女性的身心健康。现已明确[1]人乳头状瘤病毒(human papillomavirus,HPV)持续感染是引发女性宫颈癌发病的主要原因,其中HPV E6/E7蛋白的持续表达在宫颈癌发生发展过程中起重要作用,本文对 HPV E6/E7mＲNA 的研究进展进行综述，来探讨人乳头状瘤病毒(HPV)E6／E7mRNA 检测在宫颈病变研究中的价值。

简介：TostudytheexpressioncharacteristicofJapaneseencephalitisvirus(JEV)prMEandEproteinsandtheefficacyofDNAimmunizationbydifferentrecombinantplasmidscontainingJEVprME(2001bp)andE(1500bp)genes,tworecombinants(pJMEandpJE)containingJEVprMEandEgenesfusedwithFLAGwereconstructedandthentransfectedintoHepG2andCOS-1cellsbylipnsomefusion.TheexpressionfeatureofFLAG-prME(about72kDa)andFLAG-E(about54kDa)proteinsintransfectedcellswereanalyzedbyWesternblotandtwoantibodysystems(anti-FLAGandanti-E).BALB/cmicewereimmunizedwith100μgoftwokindsofrecombinantsbyintramuscularinjection,andJEVJaGAr-01strains(10^5PFU/100μl)weregiventoBALB/cmicebyintraperionealinjection3wkaftertwiceDNAimmunizationbyalethalviruschallenge.BALB/cmicewereobservedfor21daysafterchallenge.80%plaquereductionneutralizationtestwasperformedtotitrateneutralizationantibodybeforeandafterviralchallenge.ItwasfoundthattheexpressionofproteinsassociatedwithpJMEandpJEwasdeterminedintransfectedcellswithanti-FLAGandanewproteinof11kDawasdetectedinHepG2andCOS-1cellstransfectedwithpJME.OnlyE(53kDa)proteinwasidentifiedastransfectedwithpJMEusingantiE.HigherlevelofneutralizationantibodiesandtheefficacyofprotectiveimmunitywereinducedwithpJMEimmunization,andweresimilartothoseinducedbyinactivatedJapaneseencephalitisvaccine,butwerebetterthanthoseinducedwithpJE.ItconcludesthattheexpressionlevelfromprMtoEproteinsofJEVisdifferentinvitro,andtheinvitroexpressionefficiencyofpJMEwasbetterthanthatofpiE.FLAG-prMEproteinexpressedbypJMEcouldbecleavedbypeptidasefromhost.TheefficacyofDNAimmunizationiscorrelatedtotheexpressioncharacterizationofrelatedproteinsexpressedinvitro.

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简介：【摘要】目的：探究维生素E辅助钙剂治疗妊娠高血压的疗效。方法：选择本院2021.12——2022.12期间临床收诊妊娠高血压患者共计66名，设为实验对象并基于“平行对照分组法”分出常规组、实验组，每组33名。常规组应用常规硫酸镁联合硝苯地平治疗，实验组增加应用维生素辅助钙剂治疗。比较两组临床疗效、血压水平。结果：实验组临床疗效高于常规组，P＜0.05。实验组血压水平低于常规组，P＜0.05。结论：妊娠高血压病人通过维生素E辅助钙剂治疗，血压明显下降，治疗效果好，建议普及。

简介：InordertorevealvariationandrevolutionofNPgenesofhumanavianH_5N_1influenzavirusstrains,theNPgeneofahumanavianH_5N_1influenzavirusstraininGuangdongwassequencedandtheglobalNPgenesofstrainswereretrieved.ThesequenceswereanalyzedbyDNAStar5.0,andtheevolu-tionaryspeedwasstudiedwithreferencetotheepidemiologicaldata.ItwasfoundthatNPgenesof45strainsduring1997-2006werehomologicallyclassifiedintothreegroups:strainsin1997-1998,strainsin2004-2005andstrainsfrom2003to2006.Therewere35substitutionsinNPsinallstrainsaccountingforaratioof7.03%(35/498).Anadditionalglycoproteindomain(NGT_(430-432))wasfoundinNPgenesinthestrainsof2003-2006,themutationofN_(370)SinGD-01-06resultedinoccurrenceofonemoreglyco-proteindomain(NES_(368-370)).Inthesynonymousvariation,K_svaluesinNPwere2.03×10~(-5)-2.55×10~(-5)Nt/dandK_avaluesinNPwere1.58×10(-6)-3.10×10~(-6)Nt/d.Theredidn′texistobviouslyselectivepres-sure.Anadditionalglycoproteindomainineverystrainof2003-2006andonemoreinstrainGD-01-06mightchangetheantigenicityofhumanavianH_6N_1influenzavirus.ThevariationonhumanavianH_5N_1influenzastrainsoccurredfrequentlyinthenaturalworld,whichwouldresultinhighprobabilityofhu-man-humantransmissionalongwiththenaturalevolutionofthevirus.

简介：ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.

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简介：摘要目的：为先兆流产患者提供寿胎丸治疗并分析治疗效果及其对患者血清中雌二醇及孕酮的影响；方法：选取2018 年1 月至2018 年12 月于本院接受治疗的早孕先兆流产患者100 例。将实施寿胎丸加地屈孕酮治疗的设定为实验组（50 例），将实施地屈孕酮的设定为对照组（50 例），对比两组患者在治疗有效率、血清孕酮和雌二醇浓度以及人绒毛膜促性腺激素浓度等方面的差异；结果：实验组各项指标均显著优于对照组（P<0.05 ）；结论：为先兆流产患者提供寿胎丸治疗，能够有效提升患者血清孕酮、雌二醇和人绒毛膜促性腺激素浓度水平，改善黄体功能。

简介：【摘要】目的：探究分析5E康复护理模式对肺癌患者肺功能及生活质量的应用效果。方法：选取本院胸外二科（2022年5月-2023年5月）收治的肺癌患者共60例，将其进行随机分组，分为观察组和对照组两组。对照组30例，应用常规护理,观察组30例，应用5E康复护理模式。通过相关统计学来对比分析两组患者的肺功能及生活质量。结果：观察组肺功能及生活质量明显比对照组好，（P＜0.05）。结论：5E康复护理模式对肺癌患者的肺功能及生活质量具有很大的积极意义。该护理方式值得在临床当中推广及应用。

简介：摘要：在新冠肺炎抗疫过程中，为切断传播途径，避免交叉感染，保护医护人员自身安全，医护人员在接触确诊患者或疑似患者时，必须佩戴医用N95口罩，做好严格的隔离防护。因N95口罩密闭性能良好，紧贴面部，且医护人员佩戴时间一般较长，导致面部压力性损伤时有发生。为预防面部压力性损伤的发生，特提出几种预防小技巧。本文中4种方法各有优势，其中赛肤润液体敷料成本虽高，但使用方法简单，对皮肤刺激性相对小，预防压力性损伤效果好，容易被一线医护人员所接受。

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简介：摘要 目的 比较前交叉韧带（anterior cruciate ligament，ACL）重建术中股骨侧I.D.E.A.L.定位和解剖定位两种方式的短期临床效果。方法：选取2019年10月至2020年10月我院行ACL重建手术的43名患者。随机分为两组：股骨侧I.D.E.A.L.定位21例、解剖定位22例，均选择单束重建。比较两组术前术后12月Lachman试验、轴移试验阳性率；术后3月、6月、12月IKDC评分、Lysholm评分及术后6月重建移植物与胫骨平台夹角。结果：两组术后Lachman试验、轴移试验阳性率较术前显著降低（

简介：【摘要】目的：探讨分析以5E理念为基础的综合康复护理对股骨粗隆间骨折患者的影响。方法：选择2018年3月~2019年12月我院收治的股骨粗隆间骨折患者108例，按照随机数字表法将患者分为观察组55例、对照组53例。对照组患者给予常规护理对策，观察组患者在对照组基础上给予以5E理念为基础的综合康复护理对策。比较两组患者术后疼痛程度、术后恢复情况及术后髋关节功能情况。结果：术后1周两组患者VAS评分较术前显著降低（P

简介：【摘要】2020年初，在湖北武汉爆发了新型冠状病毒感染的肺炎疫情，全国30个省启动了重大突发公共卫生事件一级响应。绵竹市为进一步做好全市医疗卫生应对呼吸道相关重大传染病的防控工作，提高对新冠肺炎防治水平和应对能力,切实有效地防止新冠肺炎疫情的扩散蔓延,提出了建立疫情防控的“1+6+N”三级医疗救治体系。本文以孝德卫生院围绕“1+6+N”开展的系列工作为例，阐述了“1+6+N”体系在疫情防控中的作用。

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简介：TheaimofthisstudyistoinvestigatecyclinE,pl6inkdaandki67aspossiblediagnosticbiomarkersforcervicalpreneoplasiausingcervicalexfoliated-cellspecimens,andevaluatethesignificanceforscreeningpatientsathighriskofdevelopingcervicalcarcinoma.TheexpressionofcyclinE,pl6inkdaandki67wasexaminatedin78cervicalexfoliatedepithelialspecimensdiagnosedasatypicalsquamouscellsofundeterminedsignificance(ASCUS)(12cases),cervicalintraepithelialneoplasia(CIN)oftype1(17cases),CIN2_3(38cases)andinvasivecarcinoma(11cases)usingimmunohistochemicalanalysis,andsimultaneously,theDNAstatusofhumanpapillomavims(HPY)type16/18wasdetectedbypolymerasechainreaction(PCR)usingtypespecificprimers,cyclinE,pl6inkdaandki67werealloverexpressedinCINsandinvasivecarcinoma,comparedwithlittleexpressioninASCUS(P<0.005).OverexpressionofcyclinEwasobservedinCIN1(94.1%,X^2=21.16,P<0.01),andp16inkdaandki67wereoverexpressedininvasivecarcinoma(100%and90.9%respectively).Thedegreeofpl6inkdaandki67expressioncorrelatedwellwiththatofepitheliallesions(P<0.005).HPV16/18infectionwasassessedinC1Nsandinvasivecarcinomasamples,andrevealedasignificantrelationshipwiththedegreeofcervicalepitheliallession.Theexpressionlevelofpl6inkdaandki67seemedmorecloselyassociatedwithHPVI6infectionthanthatofcyclinE(rs=1.0vsrs=0.4).Only1caseinCINIanddcasesinCIN2-3ofHPV18positivesamplesweredetected.Thereforenostatisticalsignificancewasfoundbystatisticalanalysis.OverexpressionofcyclinE,pl6inkdaandki67inCINsandinvasivecarcinomacellsdemonstratesthepotentialuseofcyclinE,pl6inkdaandki67asdiagnosticbiomarkersforHPV-relatedcervicalneoplasticlesions.Inaddition,thistechniquecanbeusedforscreeningpatientsathighriskofdevelopingcervicalcarcinoma.

简介：ThegenomeoftheenterohemorrhagicEscherichiacoliO157:H7EDL933contains177“O”-islands(OIs).TostudytheirpotentialcontributiontotheO157-specificpathogenicity,wesurveyedthedistributionof22OIsbyPCRandDNAhybridizationin17isolatesofShigatoxinproducing(Stx-positive)E.coliO157:H7,andcomparedwiththeirdistributionin21isolatesofStx-negativeE.coliO157and21isolatesofnon-O157entericpathogens.Fourteenof22OIswerepresentinnon-O157entericpathogensanalyzed.Eightof22OIswerefoundonlyinthe17Shigatoxin-(Stx)positiveE.coliO157:H7isolates,buttheywereabsentfromthe21Stx-negativeE.coliO157:NMandO157Hundisolatestested.Amongthe8OIs,onlyOI43orOI48wereexclusivelydetectedinStx-positiveE.coliO157:H7,absentfromneitherofStx-negativeE.coliO157andnon-O157entericpathogens,suchasSalmonella,ShigeUa,Citrobacter,Vibriocholera,enteropathogen-icE.coli(EPEC),enteroadherentE.coli(EAEC),enteroinvasiveE.coli(E1EC)andenterotoxingenicE.coli(ETEC).TheOI43andOI48are83kbinsizeandidenticalinDNAsequences,whichencodegenesforurease,telluriteresistanceandadherence.ByanalyzingtheirjunctiongeneswithPCRandDNAhybridization,wefoundthat21ChineseisolateshaveOI48only.However,for7Japanesepatientisolates,4haveOI43and3haveOI48;forAmericanisolates,2havebothofO143andOI48,2haveOI48only.ThesedataconfirmedthehighlyplasticityofthepathogenicE.coligenome.TheuniquepresenceofOI43/OI48inStx-positiveE.coli0157:H7denotesitscriticalroleinthepathogenicityspecifictothispathogen.

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