简介：ToestablisharapididentificationmethodforcommonpathogenicbacteriaonthebasisofmolecularbiologyandtoconstructapreliminaryPolymeraseChainReaction-CapillaryElectrophoresis-RestrictionFragmentLengthPolymorphism(PCR-CE-RFLP)databaseofbacteriaisolatedfromclinicalspecimensfrequently,183strainscollectedfromclinicalsamplesbelongingto12generaand19specieswhosebiochemicalcharacterizationscorrespondedtothetypicaloneswereexamined.ThegenomicDNAswereamplifiedbytwopairsoffluorescencelabeledprimersaimingat16SrRNAgeneand16S-23SrRNAspacerregiongenerespectivelyatthesametime.PCRproductswerethendigestedbyrestrictionendonucleaseHaeⅢin-completelybeforetakingcapillaryelectrophoresis.TheresultswiththePCR-CE-RFLPpatternsof16SrRNAgeneswerejustalikewithinsomegenera,butwhenitcomesto16S-23SrRNAspacerregiongenes,eachbacteriumshowedauniquepattern,whichcanbedistinguishedfromeachothereasily.ItseemsthatPCR-CE-RFLPpatternsof16SrRNAgenecouldonlybeusedtoclassifythebacteriaintofamilylevel,whereasthedataof16S-23SrRNAspacerregiongenecouldbeutilizedtoidentifythewholemicroorganismsaspreciselyasthespecieslevel.Inspiteofthedataofthespacerregiongenealonecanbesufficientlytoverifythewholebacteria,weinsistthatthe16SrRNAgenecouldbeofsomeassistantincasethatthereshouldbelotsoffamiliesofbacteria,inwhichsomesimilarones,withthesameRFLPdataof16S-23SrRNAspacerregiongene,maycoexist.ThisstudyprovesthattheutilityofPCR-CE-RFLPisaconvenient,rapidmethodtoidentifypathogenicbacteria,andisalsoaquickdiagnosismeasureforapplicationtoclinicaluse.

简介：TherelationshipbetweenembBmutationofMycobacteriumtuberculosisandethambutol(EMB)resistanceoftheclinicalisolatesoftuberculouspatientsinChinawasinvestigatedbyreversedotblothybridization(RDBH)inadditiontoevaluatingtheclinicalvaluewithapplicationofPCR-RDBHtechniquetodetectEMBresistance.Inthepresentstudy,thegenotypesofthe258bpfragmentsofembBgenesfrom196clinicalisolatesofM.tuberculosiswereanalysedwithRDBHandDNAsequencing.Itwasdemonstratedthat60outof91phenotypicallyEMB-resistantisolates(65.9%)showed5typesofmissensemutationsatcodon306ofembBgene,resultinginthereplacementoftheMetresidueofthewildtypestrainwithVal,IleorLeuresidues.Inthesemutations,theGTPmutation(38/91,41.8%)andtheATAmutation(16/91,17.6%)werethemostencounteredgenotypes.TheembBmutationatcodon306couldalsobefoundin69isolatesofphenotypicallyEMB-sensitivebutresistanttootheranti-tuberculousdrugs,butnosuchgenemutationcouldbefoundin36strainsofdrug-sensitiveisolates.Meanwhile,theconcordancewiththeresultsofDNAsequencingforonewide-typeprobeand5probesforspecificmutationswas100%.ItwasconcludedthattheEMB-resistanceoccurringinmostM.tuberculosisisduetoappearanceofembBmutationatcodon306,andthePCR-RDBHassaywasprovedtobearapid,simpleandreliablemethodforthedetectionofgenemutations,whichmightbeagoodalternativeforthedrug-resistancescreening.

简介：【摘要】目的 探讨适应性统计迭代（adaptive statistical iterative reconstruction,ASIR）、滤波反投影(FBP)两种重建技术对胸部CT图像质量的影响。方法 80例患者均应用西门子64排CT行胸部CT平扫。分别用ASIR、FBP两种重建算法对原始数据进行重建，重建层厚1mm，层间距0.5mm。测量记录信噪比（SNR）、图像噪声、对比噪声比（CNR）。对肺窗和纵隔窗进行图像质量评价。对比分析两种不同重建算法的定性定量参数。结果 两组图像的噪声值分别是35.6±14.5，52.5±18.6，与FBP组比较，ASIR组噪声降低了31.4%，ASIR组的SNR和CNR明显高于FBP组（P＜0.05）。且ASIR组肺窗及纵隔窗图像质量较FBP组高，差异有统计学意义（P＜0.05）。结论 与FBP重建算法相比，ASIR可明显降低图像的噪声，提高图像质量。

简介：摘要：人主要依靠声音与他人进行交流，而交流的过程中都会传递一些情感，不同的情感则就会有不同蕴藏着不同的讯息，对人能产生不同的作用。因此，进一步加强语音情感识别则就变得十分重要，其是识别人情感变化重要“工具”，是加强人际交流的不可缺的基础。本文重在研究基于语音情感的心理咨询和性格分析算法，侧重分析情感信号识别处理、人类语音情感特征及人工智能识别系统，帮助人们更好的进行交流，并做出准确预测，最后在给出不同的相关的心理分析与建议。