简介：ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.

简介：摘要：目的 分析20例小儿高热惊厥的急救及护理对策。方法 选择2018年~2019年来我院儿科治疗的20例惊厥患儿，对其进行急救措施，从而展开分析、总结。结果 20例惊厥患儿中，痊愈17例痊愈，2例转院，1例存在后遗症。结论 实施小儿惊厥护理之后，不但需要灵活掌握急救对策，还应相应的实施护理，从而减小由于高热产生的脑损伤，进而提高惊厥患儿的成功几率，可以在较短时间内帮助患儿度过危险期，预防并发症的产生。

简介：

简介：

简介：

简介：摘要：目的：研究在针对神经外科患者的护理中，实施流程优化的价值和作用。方法：选取某院2020年1月-2022年2月期间收治的神经外科患者作为研究对象，患者总数为168例，其中84例患者在护理工作开展过程中未实施流程优化，该组患者作为对照组；其中84例患者在护理工作开展过程中实施流程优化，该组患者作为观察组，比较两组患者在接受护理过程中的效果。结果：观察组患者在接受护理过程中，护理安全评分、病房管理评分、护理满意度都明显优于对照组，且差异具有统计学意义。结论：在针对神经外科患者的护理中，实施和应用流程优化具有较为积极的作用，能够让整个护理过程更加安全，让患者得到更好的恢复。

简介：Toinvestigatetheroleofnegative-regulatoryfactorsA20,IRF-4andTRAF4ofthetoll-likereceptor(TLR)signalpathwaysinimmunologicalpathogenesisofKawasakidisease(KD),48childrenwithKawasakidisease,16childrenwithinfectiousdisease(ID)and16age-matchedhealthychildrenwerestudied.Reverse-transcriptionPCR(RT-PCR)andreal-timePCRwereusedtoevaluatetheexpres-sionlevelsofnegative-regulatoryandeffectivefactorsintoll-likereceptor4(TLR4)signalpathwaysandproinflammatoryfactorsinperipheralbloodmonocyte/macrophage(MC).Inthisstudy,expressionlevelsofTLR4,MD-2,MyD88,IRAK-4,TRAF6,TAK1,andTAB2mRNAinKDgroupweredetectedtobeelevatedsignificantlyduringacutephaseofKD.Transcriptionlevelsofnegative-regulatoryfactorsA20,IRF-4andTRAF4mRNAinKDorIDpatientsincreasedremarkably.However,expressionsofIRF-4andTRAF4inKDpatientsweredetectedtobelowerthanthatinIDpatients,exceptthattran-scriptionlevelsofA20werefoundtobehigherthanthatinIDpatients.Simultaneously,expressionsofproinflammatorycytokinessuchasL-1β,IL-6andTNF-αinKDpatientsweresignificantlyelevatedcom-paredwiththoseinIDpatients.Furthermore,itwasfoundthatstimulationoflipopolysaccharide(LPS)remarkablyup-regulatedtheexpressionsofnegative-regulatoryfactorsA20,IRF-4andTRAF4inKDpa-tientsorhealthyvolunteers.ThemRNAlevelsofallthethreefactorsinKDpatientswerefoundtobelowerthanthatinthelatter.Inaddition,transcriptionlevelsofIRF-4andTRAF4inKDpatientswithcoronaryarterylesion(KD-CAL~+)weredetectedtobelowerthanthoseinKDpatientswithoutcoronaryarterylesion(KD-CAL~-)duringacutephase,whilethatofA20inKD-CAL~+groupwerelowerthanthatinthelatter.AndthelevelsofexpressionsofproinflammatorycytokinesinKD-CAL~+groupwerefoundtobehigherthanthoseinKD-CAL-group(P<0.01).Thesefindingssuggestthataberrantexpressionofnegative-regulatoryfactorsofTLRssignalpathwaysmaybeinvolved

简介：

简介：摘要 目的 探究心内科护理单元对急性心肌梗死病人应对方式、自护能力及满意度的影响。方法 选取2018年12月至2019年12月在我院心内科进行治疗的AMI患者108例，将其分为常规组（A组）给予常规护理，单元护理组（B组）给予单元护理，比较其应对方式、自护能力及满意度。结果 B组应对方式评分优于A组，且B组自护能力总分为（123.14±4.99）分，与A组（110.74±4.92）分及治疗前（98.14±3.71）分均存在明显差异，满意度也高于A组。结论 心内科采用护理单元方式对急性心肌梗死病人进行护理，对提高患者应对方式、自护能力均有明显益处，同时可提高患者对护理的满意度，值得推广。