简介:Theaimofthisstudyistoinvestigatewhetherthreemononucleotidepolymorphismsatthelocus-1082,-819and-592inthepromoterregionoftheIL-10geneareassociatedwithchronicseverehepatitis.TheIL-10-592andIL-10-1082polymorphismsweregenotypedbypolymerasechainreaction-restrictionfragmentlengthpolymorphismanalysis(PCR-RFLP)whilepolymerasechainreaction-se-quencespecificprimer(PCR-SSP)assaywasusedtotesttheIL-10-819polymorphism.Thepolymor-phismsofIL-10-1082,-819and-592genesweredetectedin98patientswithchronicseverehepatitis(CSH),478patientswithchronichepatitisB(CHB),223asymptomatic(chronic)HBVcarriers(ASC)and267patientswithself-restrictedHBV.Therewassignificantdifferenceofthepolymor-phismsofIL-10-1082,IL-10-819andIL-10-592genesbetweenCSHgroupandothergroups.Thefre-quencyofAAgenotypeatIL-10genepromoter-1082locusinchronicseverehepatitispatientswashigherthanthatinasymptomaticHBVcarriers(X~2=13.314,P=0.001),andself-restrictedHBVpatients(X~2=13.545,P=0.000);thefrequencyofCCandACgenotypeatIL-10genepromoter-592locusinchronicseverehepatitispatientswashigherthanthatinchronichepatitispatients(X~2=15.970,P=0.000)(X~2=20.414,P=0.000),asymptomaticHBVcarriers(X~2=21.283,P=0.000)(X~2=28.309,P=0.000)andself-restrictedHBVpatients(X~2=17.047,P=0.000)(X~2=16.528,P=0.000);thefrequencyofTCgenotypeatIL-10genepromoter-819locusinchronicseverehepatitispatientswashigherthanthatinchronichepatitispatients(X~2=58.961,P=0.000),asymptomaticHBVcarriers(X~2=53.255,P=0.001)andself-restrictedHBVpatients(X~2=39.616,P=0.001).Sointerleukine-10genepolymorphismwasassociatedwiththechronicsevereheoatitis.