简介:为研究温度对岩扇贝(Crassadomagigantea)幼贝生长的影响,进一步了解其随温度变化的生长规律,在室内循环水控温养殖条件下,设置10℃、12.5℃、15℃、17.5℃四个温度梯度,在两个月的培养周期中对不同试验温度岩扇贝幼贝的生长情况进行观测。结果表明:随试验温度升高,其生长速率呈先升高后下降趋势。单因素方差分析显示温度对其生长速率影响显著(P<0.05),最适生长温度为15℃,该条件下幼贝的壳高平均日生长量显著高于其他三组,壳长与湿重的平均日生长量与17.5℃实验组无显著性差异,但显著高于前面两组。试验范围内,温度变化对幼贝存活率影响不显著(P>0.05)。
简介:The12ththePacificAsianMarginalSeas(PAMS)/JapanandEastChinaSeaStudy(JECSS)(PAMS/JEC-SS)Workshopwasheldon22-24November2003inHangzhou,China.Morethan100scientistsattendedthisworkshop.Therewere57oralpresentationsand43postpresentations,groupedinto7themes,namely,(1)StudiesoftheSouthChinaSea(2)StudiesoftheBohai,HuanghaiandEastChinaSeas
简介:WeusedearthquakecatalogsrecordedbyGuangdongSeismologicalNetworkfrom2008to2014toresolvethespatialvariationsofb-valuesinthecoastalareaofGuangdong,particularlyinthreekeyresearchareas(Yangjiang,Heyuan,andoffshoreNanaoIsland)withstrongseismicity.Ourresultsrevealedthatb-valuesexhibitedsignificantspatialvariations,andzoneswithlowb-valuescouldindicatethemostlikelyseismogenicareaoflargeearthquakes.Weobservedthreeclearlowb-valuepatchesintheoffshoreNanaoIsland.Wefoundadistincthighb-valuepeakatthedepthof11kmandtwominimumpeaksatabout14and7–8kmintheYangjiangarea.Theoverallb-valuesgenerallydecreasewithdepthintheHeyuanarea.Thespatialvariationsofb-valuesreflecttectonicanomalies;thatis,the‘low-high-low’distributionofb-valuesintheoffshoreNanaoIslandandtheYangjiangareamayindicatetheanomalyofthecrustalstructurewithaweaklayer.Theb-valuesofreservoir-inducedseismicityareobviouslylowerthanthatinducedbytectonism.Thisfindingindicatesthatthereservoirareaisgenerallyathighstressstateundertheconditionofhighporepressure.Weinferredthatlargeearthquakesmightbepronetooccurat10–12kmdepthintheoffshoreNanaoIsland,at12–15kmdepthintheYangjiangarea,andatthelowerpartoftheseismicactivityzoneintheHeyuanarea.Moreover,theupstreamareaoftheXinfengjiangreservoiristhemostlikelyareaoffuturelargeearthquakesintheHeyuanarea.
简介:SequencevariationofpartialcytochromebgenesbetweentwoCoiliaspecies,C.ectenesandC.mystus,wasinvestigated.Ofthe402nucleotides,twenty-seven(6.72%)arepolymorphicandallaresynonymoussubstitutions.Atthethirdpositionsofgeneticcondonofcytochromebgene,thetwospeciesshowanextremeanti-Gbias(<4%)andapronouncedbiastowardsAandC(>68%).Thereisnoaminoacidsequencedivergencebetweenthepartialcytochromebgenesofthetwospecies,indicatingaclosegeneticrelationshipbetweenthem.Thek-2pgeneticdistanceofpartialcytochromebsegmentofthetwospeciesis0.072,suggestingthatthespecieswereseparated3.6Maago,inthemiddlePliocene.Ourresultrevealsthatthecytochromebgeneisanappropriatemarkerforstudiesofpopulationgeneticstructuresandphylogeographicpatternsofthetwospecies.
简介:Effectsofthemixtureofβ-ecdysoneandpolypodine-B(2:1W/W)onthemoultingandgrowthofprawn,Penaeusorientalisswerestudiedinlaboratorybyrearingtheanimalwiththeaddedcompounddiets.Theresultsshowedthattheadditionofthemoultinghormone(M.H)mixturetocompounddiets,from1.33×10-6to30×10-6,couldincreasemoultingactivityandgrowthrateoftheprawntodifferentextents,amongwhichthe5.33×10-6-10.67×10-6additionhadstatisticallysignificanteffects.However,60×10-6addeddietscausedmoultingandgrowthinhibitionsandcuticlethickening.Theanalysesofprotein,waterandashcontentsoftheprawnshowednoremarkabledifferencesbetweenhormonedosedanimalsandthecontrol.
简介:HepatitisBvirus(HBV)isasignificantglobalpathogenandefficientcureforHBVpatientsisstillachallenginggoal.Wepreviouslyreportedthatacidicmucopolysaccharidefromstichopusjaponicusselenka(SJAMP)couldinhibitHBsAgandHBeAgexpressioninvitro.However,thepotentialanti-HBVeffectsofSJAMPinvivohavenotyetbeenexplored.Inthisstudy,weshowthatSJAMPexhibitspotentanti-HBVactivityinHBVtransgenicmiceinadose-dependentmanner.Specifically,sixtyHBVtransgenicmaleBALB/cmicewererandomlyselectedtoreceivethetreatmentofPBS,lowdoseSJAMP(30mgkg-1),middledoseSJAMP(40mgkg-1),highdoseSJAMP(50mgkg-1)andIFN(45IUkg-1)for30d.SJAMPtreatmentsuppressedserumHBV-DNA,andliverHBsAgandHBcAglevelsinHBV-transgenicmice.ThepresentstudyhighlightsthepotentialapplicationofSJAMPinHBVtherapy.
简介:我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中,羧甲基纤维素酶(CMCase)在培养的细胞。短梗霉98的纯化至均一,与酶的最大产量为4.51U(mg蛋白)-1。SDS-PAGE分析表明,纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度,比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6,和活动的个人资料被稳定在一个范围内的酸度(pH5,0-6.0)。这种酶被激活Na+,Mg2+,Ca2+,K+,Fe2+和Cu2+,然而,它是由Fe3+,Ba2+,Zn2+,Mn2+和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1(mg蛋白)1,分别。只有大小不同的低聚糖,羧甲基纤维素(CMC)释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆,其中包含一个开放阅读框(eu978473)意义。推导的蛋白质含有酶超家族的保守结构域(糖基水解酶家族5)。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f,这与从克隆的基因推导一致。这表明,纯化的酶是由克隆纤维素酶基因在酵母编码。
简介:Huanghe河三角洲是世界的大河之一,自从最后,Huanghe河里的Huanghe河三角洲paleoenvironmental进化是一个热问题冰川。基于联合AMS14核心DYZK1沉积标明日期在的C在亚底部侧面上Huanghe河三角洲和声学的顺序沉没了,phytolith分析在96件沉积样品上被执行。谷物尺寸参数,磁性的危险性与biostratum的垂直变化被相结合重建在Huanghe河三角洲的paleo沉积、气候的条件。在索引参数有一条重要垂直变化法律的学习结果表演,和Huanghe河三角洲的那沉积环境经历了河的facies-sealand转变的进化进程外形潮汐的完全外形浅海的faciesdelta外形自从26.0kaB。P...phytolith分析结果如下。第一,phytoliths被划分成矛尖状,光滑伸长,多刺伸长,一种特殊格律,长矩形,bulliform和其它类型。在不同sedimental环境,phytolith内容定期变化,显示phytolith在沉积环境有的一样装配稳定性的某个度。第二,矛尖状,phytoliths的光滑伸长和多刺伸长范畴在潮汐的扁平的外形和三角洲免职有更大的内容,当时一在浅海的外形的更小的内容和河的外形环境。通过变化系数的比较分析,第三,在沉积的主要phytolith范畴的内容在sealand转变外形和河的外形免职有一个更大的变化振幅,当在tidalflat外形和三角洲外形免职是更稳定的时。
简介:以卤虫无节幼体(36h)和成虫(16d)为例,在温度为15±3℃、盐度为30的条件下,通过单因子静态急性毒性试验方法,分别研究了Cu2+、Mn2+和Zn2+对卤虫的无节幼体和成虫的单一毒性效应;在此基础上,以卤虫卤虫无节幼体(36h)为例,在相同试验条件下,通过等毒性溶液法,研究了Cu2+、Mn2+和Zn2+对卤虫的联合毒性效应。试验结果表明:卤虫无节幼体对三种金属离子的耐受能力均高于成虫,Cu2+﹑Mn2+和Zn2+的48hLC50分别是4.0、27.19和115.95mg/L,而成虫Cu2+﹑Mn2+和Zn2+的48hLC50仅为1.31、6.86和34.23mg/L;且对两个时期的卤虫,三种金属离子毒性由强至弱均为Cu2+>Mn2+>Zn2+;三种重金属对卤虫无节幼体和成虫的安全浓度(SC)分别是0.4、2.72、11.6、0.13、0.69和3.42mg/L。联合毒性试验结果表明Cu-Mn、Cu-Zn和Mn-Zn均对卤虫无节幼体表现为拮抗作用。