简介:AninteractivetooltovisualizetheK-stringcompositionoflongDNAsequencesincludingbacterialcompletegenomesisdescribed.Itisespeciallyusefulforexploringshortpalindromicstructuresinthesequences.TheSeeDNAprogramrunsonRedHatLinuxwithGTK+support.Itdisplaystwo-dimensional(2D)orone-dimensional(1D)histogramsoftheK-stringdistributionofagivensequenceand/oritsrandomizedcounterpart.ItisalsocapableofshowingthedifferenceofK-stringdistributionsbetweentwosequences.TheCsourcecodeusingtheGTK+packageisfreelyavailable.
简介:WehaverecentlyclonedapathogeninducibleblastresistancegenePi-khfromtheindicaricelineTetepusingapositionalcloningapproach.Inthisstudy,wecarriedoutstructuralorganizationanalysisofthePi-khlocusinbothindicaandjaponicaricelines.A100kbregioncontaining50kbupstreamand50kbdown-streamsequencesflankingtothePi-khlocuswasselectedfortheinvestigation.Atotalof16genesinindicaand15genesinjaponicawerepredictedandanno-tatedinthisregion.TheaverageGCcontentofindicaandjaponicagenesinthisregionwas53.15%and49.3%,respectively.Bothindicaandjaponicasequenceswerepolymorphicforsimplesequencerepeatshavingmono-,di-,tri-,tetra-,andpentanucleotides.SequenceanalysisofthespecificblastresistantPi-khalleleofTetepandthesusceptiblePi-khalleleofthejaponicaricelineNipponbareshoweddifferencesinthenumberanddistributionofmotifsinvolvedinphosphorylation,resultingintheresistancephenotypeinTetep.