学科分类
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10 个结果
  • 简介:流行性感冒A病毒(H1N1)2009,一个新猪起源流行性感冒A病毒,世界范围地被散布了并且引起了大公共害怕。高产量transcriptomicsproteomics方法现在正在被使用识别H1N1H1N1主人相互作用。这篇文章H1N1诊断,处理H1N1病毒主人相互作用考察最近transcriptomicsproteomics研究,到为进一步理解感染机制并且控制H1N1传播提供一些帮助。

  • 标签: 蛋白质组学 基因表达谱 A型流感病毒 转录组学 相互作用 控制传输
  • 简介:流行性感冒A病毒(H1N1),人地方紧张一个基因分类,鸟并且猪流感,穿过种类障碍到人并且显然获得了人能力到人传播。因为NS1蛋白质禁止抗病毒干扰素/生产,H5N1子类型一些紧张高度剧毒。另一蛋白质NS2调停到通过出口细胞质从原子核病毒ribonucleoprotein出口信号。在这份报纸,我们学习了H1N1子类型这些蛋白质结构功能关系并且决定了他们致病力原因。我们结果证明非保守变化稍微稳定了或使动摇NS1或NS1-dsRNA建筑群结构域,稍微因此增加了或减少NS1蛋白质并且因而函数提高了或减少H1N1病毒致病力。不同紧张NS2蛋白质不同领域带了非保守变化,导致功能细微损失。这些变化稍微减少了病毒致病力。因此,结果证实这些病毒蛋白质结构功能关系。

  • 标签: H5N1亚型 蛋白质结构 NS2 A型流感病毒 高致病性 生理盐水
  • 简介:Thesurfaceglycoproteinhemagglutinin(HA)helpstheinfluenzaAvirustoevadethehostimmunesystembyantigenicvariationandisamajordrivingforceforviralevolution.Inthisstudy,theselectionpressureonHAofH5N1influenzaAviruswasanalyzedusingbioinformaticsalgorithms.Mostoftheidentifiedpositiveselection(PS)siteswerefoundtobewithinoradjacenttoepitopesites.SomeoftheidentifiedPSsitesareconsistentwithpreviousexperimentalstudies,providingfurthersupporttothebiologicalsignificanceofourfindings.ThehighestfrequencyofPSsiteswasobservedinrecentstrainsisolatedduring2005–2007.PhylogeneticanalysiswasalsoconductedonHAsequencesfromvarioushosts.Viraldriftisalmostsimilarinbothavianandhumanspecieswithaprogressivetrendovertheyears.OurstudyreportsnewmutationsinfunctionalregionsofHAthatmightprovidemarkersforvaccinedesignorcanbeusedtopredictisolatesofpandemicpotential.

  • 标签: H5N1病毒 选择压力 血凝素 演变 系统发育分析 流感病毒
  • 简介:在这研究,L1蛋白质定序的人乳头状瘤病毒(HPV)107种类型从可得到数据库,这些HPVL1蛋白质原子本地化信号(NLS)被获得被生物信息分析分析并且预言。从107种类型,39种类型NLS被PredictNLS软件(由两部组成NLS35种类型单音深裂NLS4种类型)预言。留下HPV类型NLS象NLS一般规则一样根据特征已经预言NLS相同被预言。根据结果,HPVL1蛋白质107种类型NLS被分类进15个范畴。一样NLS范畴HPVL1蛋白质不同类型能分享类似或一样nucleocytoplasmic运输小径。他们可能被用作一样目标阻止并且对待HPV感染不同类型。结果也证明生物信息技术能被用来分析并且预言蛋白质NLS。

  • 标签: 疾病预防 核信号 人类乳头瘤病毒 病毒感染
  • 简介:Asystematicphylogeneticfootprintingapproachwasperformedtoidentifycon-servedtranscriptionfactorbindingsites(TFBSs)inmammalianpromoterregionsusinghuman,mouseandratsequencealignments.Wefoundthatthescoredis-tributionsofmostbindingsitemodelsdidnotfollowtheGaussiandistributionrequiredbymanystatisticalmethods.Therefore,weperformedanempiricaltesttoestablishtheoptimalthresholdforeachmodel.WegaugedourcomputationalpredictionsbycomparingwithpreviouslyknownTFBSsinthePCK1genepro-moterofthecytosolicisoformofphosphoenolpyruvatecarboxykinase,andachievedasensitivityof75%andaspecificityofapproximately32%.Almostallknownsitesoverlappedwithpredictedsites,andseveralnewputativeTFBSswerealsoidentified.WevalidatedapredictedSP1bindingsiteinthecontrolofPCK1tran-scriptionusinggelshiftandreporterassays.Finally,weappliedourcomputationalapproachtothepredictionofputativeTFBSswithinthepromoterregionsofallavailableRefSeqgenes.OurfullsetofTFBSpredictionsisfreelyavailableathttp://bfgl.anri.barc.usda.gov/tfbsConsSites.

  • 标签: 哺乳动物 子区域 转录因子结合位点 调控元件 基因启动子 守恒
  • 简介:Leishmaniaisassociatedwithabroadspectrumofdiseases,rangingfromsimplecutaneoustoinvasivevisceralleishmaniasis.Here,thesequencesoftencysteineproteasesoftypesA,BandCofLeishmaniamajorwereobtainedfromGeneDBdatabase.PredictionofMHCclassIepitopesofthesecysteineproteaseswasperformedbyNetCTLprogramversion1.2.Inaddition,byusingBcePredserver,differentstructuralpropertiesoftheproteinswerepredictedtofindouttheirpotentialBcellepitopes.Accordingtothiscomputationalanalysis,nineregionswerepredictedasBcellepitopes.Theresultsprovideusefulinformationfordesigningpeptide-basedvaccines.

  • 标签: 半胱氨酸蛋白酶 加利福尼亚 计算 原虫 B细胞表位 家庭
  • 简介:染色体17q21.31倒置普通结构多型首先在欧洲人口发现了900-kb。尽管倒置区域以内基因流动被假定可观压制,它关于H1(非转换顺序)H2(转换顺序)之间基因交换细节仍然不清楚这倒置haplotypes。这里,我们17q21.31区域以内描述一些基因安排之间基因交换一张精制地图。用1,546单个核苷酸多型HapMap阶段II数据,我们成功地由加入邻居树重建在欧洲样品推出了96H124H2haplotypes。而且,我们分别地与相互、非相互基因交换识别了1526条候选人道。怀有相互交换所有15个区域,haplotypes由克隆定序重建了没支持这些交换事件,建议这在某些异质接合个人在二个姐妹染色体之间交换发信号被分阶段执行错误区域引起。另一方面,与非相互基因流动越过26条道4个定序完成克隆证实这种基因交换被基因变换引起。摘要,更加作为一些基因安排之间转线路被压制了,基因变换可能17q21.31基因交换最重要机制。

  • 标签: 人类基因组单体型图 反转区 遗传 欧洲 人口 H2
  • 简介:Leptospirosisisrecognizedasthemostwidespreadzoonosiswithaglobaldistribution.Inthisstudy,theantigenicvariationinLeptospirainterrogansandLeptospiraborgpeterseniiisolatedfromhumanurineandfieldratkidneywaspreliminarilyconfirmedbymicroscopicagglutinationtestusingmonoclonalantibodies,andwasfurthersubjectedtoamplificationandidentificationofoutermembranelipoproteinswithstructuralgenevariation.Sequencesimilarityanalysisrevealedthattheseproteinsequences,namelyOmpL1,LipL32andLipL41,showednomorehomologiestooutermembranelipoproteinsofnon-pathogenicLeptospiraandothercloselyrelatedSpirochetes,butshowedastrongidentitywithinL.interrogans,suggestingintra-specificphylogeneticlineagesthatmightbeoriginatedfromacommonpathogenicleptospiralorigin.Moreover,theompL1geneshowedmoreantigenicvariationthanlipL32andlipL41duetolessconservationinsecondarystructuralevolutionwithincloselyrelatedspecies.Phylogenetically,ompL1andlipL41ofthesestrainsgaveaconsiderableproximitytoL.weiliiandL.santarosai.TheompL1geneofL.interrogansclustereddistinctlyfromotherpathogenicandnon-pathogenicleptospiralspecies.ThediversityofompLgeneshasbeenanalyzedanditenvisagedthatsequence-specificvariationsatantigenicdeterminantsiteswouldresultinslowevolutionarychangesalongwithnewserovaroriginationwithincloselyrelatedspecies.Thus,acrucialworkoneffectiverecombinantvaccinedevelopmentandengineeredantibodieswillhopefullymeettosolvethetherapeuticchallenges.

  • 标签: 钩端螺旋体 致病性 脂蛋白 基因 意蕴 进化
  • 简介:InFebruary2006,twooutbreaksofhighlypathogenicavianinfluenzaAvirussubtypeH5N1occurredinchickensintwoneighboringdistricts(firstinNandurbarandsecondinJalgaon)ofMaharashtra,India,inaspanof12days.Inthepresentstudy,theneuraminidase(NA)geneofthetwoIndianH5N1isolateswastakenintoconsiderationtofindifthetwostrainsaregeneticallysimilar.PhylogeneticanalysisoftheNAgeneshowedthattheH5N1strainsisolatedfromthetwooutbreakswerenotoriginatedfromthesamesource.ThefirstIndianisolate(Nandubar/7972/06)wasclusteredclosesttoanisolatefromchickeninVietnamin2004,whereasthesecondIndianisolate(Jalgaon/8824/06)showedresemblancetostrainsisolatedfromswaninItalyandIranin2006.Moreover,aminoacidsequenceanalysisshowedvaryinghotspotsforsubstitutionsbetweenthesetwoIndianisolates,andthreesubstitutionswerefoundatfunctionaldomainsites.Secondarystructurechangesduetothesesubstitutionswerealsoreported.ThisstudyrevealsthattheH5N1strainsisolatedfromchickensduring2006birdfluoutbreaksintwoneighboringdistrictsofMaharashtra,Indiaaregeneticallydifferent.

  • 标签: 神经氨酸酶基因 H5N1亚型 系统发育分析 禽流感疫情 印度 菌株