摘要
BACKGROUND:Studieshavedemonstratedthatbrain-derivedneurotrophicfactor(BDNF)hasadualeffectonepilepsy.However,therelationshipbetweenepilepsy-inducedbraininjuryandBDNFremainspoorlyunderstood.OBJECTIVE:Accordingtoultrastructuralandmolecularparameters,todetectthedegreeofneuronalinjuryandBDNFexpressionchangesatdifferentbrainregionsanddifferentkindlingtimestodeterminetheeffectsofBDNFonepilepsy-inducedbraininjury.DESIGN,TIMEANDSETTING:Arandomized,controlled,animalexperimentbasedonneuropathologyandmolecularbiologywasperformedattheDepartmentofPhysiologyandDepartmentofPathology,BasicMedicalCollegeofJilinUniversityin2003.MATERIALS:UltraSensitiveSPkitforimmunohistochemistry(FuzhouMaximBiotechnology,China),BDNFantibody(concentratedtype,WuhanBosterBiologicalTechnology,China),JEM-1000SXtransmissionelectronmicroscopy(JEOL,Japan),andBH-2lightmicroscope(Olympus,Japan)wereusedinthepresentstudy.METHODS:Wistarratswererandomlyassignedtocontrol(n=6),sham-surgery(n=6),andmodel(n=60)groups.Thecontrolgroupratswerenottreated;anelectrodewasembeddedintotheamygdalainratsfromthesham-surgeryandmodelgroups;anamygdalakindlingepilepsymodelwasestablishedinthemodelgroup.MAINOUTCOMEMEASURES:Pathologicalchangesinthetemporallobeandhippocampuswereobservedbylightandelectronmicroscopyat1,3,7,14,and21daysfollowingkindling,andBDNFexpressioninthevariousbrainregionswasdeterminedbyimmunohistochemistry.RESULTS:Inthemodelgroup,temporallobecorticalandhippocampalneuronswereswollenandthenucleiwerelaterallydeviated.Therewerealsosomeapoptoticneurons3daysafterkindling.Thenucleolidisappearedandthenucleiappearedbrokenorlysed,aswellasslightmicrogliahyperplasia,at7days.Electronmicroscopicobservationdisplayedchromatinaggregationinthenucleiandslightmitochondrionswelling3daysafterkindling.Injurychangeswereaggravatedat7days,characteriz
出版日期
2010年08月18日（中国期刊网平台首次上网日期，不代表论文的发表时间）
