摘要
Objective:VitaminDreceptor(VDR)mediatesvitaminDactivity.WeexaminedwhetherVDRexpressioninexcisedmelanomatissuesisassociatedwithVDRgene(VDR)polymorphisms.Methods:WeevaluatedVDRproteinexpression(bymonoclonalantibodyimmunostaining),melanomacharacteristics,andcarriageofVDR-FokI-rs2228570(C>T),VDR-BsmI-rs1544410(G>A),VDR-ApaI-rs7975232(T>G),andVDR-TaqI-rs731236(T>C)polymorphisms(byrestrictionfragmentlengthpolymorphism).Absenceorpresenceofrestrictionsitewasdenotedbyacapitalorlowerletter,respectively:'F'and'f'forFokI,'B'and'b'forBsmI,'A'and'a'forApaI,and'T'and't'forTaqIendonuclease.Seventy-fourItaliancutaneousprimarymelanomas(52.1±12.7yearsold)werestudied;51.4%werestageⅠ,21.6%stageⅡ,13.5%stageⅢ,and13.5%stageⅣmelanomas.VDRexpressionwascategorizedasfollows:100%positivevs.<100%;overthemedian20%(highVDRexpression)vs.≤20%(lowVDRexpression);absencevs.presenceofVDR-expressingcells.Results:StageImelanomas,Breslowthicknessof<1.00mm,levelIIClarkinvasion,Aaheterozygousgenotype,andAaTTcombinedgenotypeweremorefrequentinmelanomaswithhighvs.lowVDRexpression.CombinedgenotypesBbAA,bbAa,AATt,BbAATt,andbbAaTTweremorefrequentin100%vs.<100%VDR-expressingcells.CombinedgenotypeAATTwasmorefrequentinmelanomaslackingVDRexpression(oddsratio=14.5;P=0.025).VDRexpressionwasnotassociatedwithmetastasis,ulceration,mitosis>1,regression,tumor-infiltratinglymphocytes,tumoralinfiltrationofvasculartissues,additionalskinandnon-skincancers,andmelanomafamiliarity.Conclusions:WehighlightedthatVDRpolymorphismscanaffectVDRexpressioninexcisedmelanomacells.LowVDRexpressioninAATTcarriersisanewfindingthatmeritsfurtherstudy.VDRexpressionpossiblyposesimplicationsforvitaminDsupplementationagainstmelanoma.VDRexpressionandVDRgenotypemaybecomeprecisemedicinaltoolsformelanomainthefuture.
出版日期
2017年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
