摘要
EighteenisolatesofRhizoctoniasolanicollectedfrominfectedriceplantsinfourdifferentlocationsofBangladeshwerestudiedbyusingmorphologicalcharactersandmolecularmarkers.AnastomosisstudywithareferenceisolateconfirmedthatalltheisolatesbelongedtoR.solani.Significantvariationwasobservedinsclerotialsize,shapeanddistribution.Un-weightedpairgroupmethodwitharithmeticmeandendrogramconstructedbasedontheGower’sgeneralsimilaritycoefficientshowedthattheseisolatesweregroupedintofourclustersatthe0.68similaritycoefficentaccordingtomorphologicalcharacters.ClusterIwasamajorclusterconsistingof13isolates,whileclustersⅡtoⅣconsistedof1or2isolates.Analysesbyvariablenumberoftandemrepeatandamplifiedfragmentlengthpolymorphismmarkersshowedthattheisolatesweregroupedintofiveandthreeclustersatasimilaritycoefficientof0.64and0.69,respectively.Althoughmostofthevariabilitywasfoundbetweenisolatesfromdifferentregionsasexpected,significantvariationwasobservedwithintheisolatescollectedfromsimilaragro-ecologicalregions.OurresultssuggestthepresenceofdifferentracesofR.solaniwithinthesamelocalgeographicregions.
出版日期
2016年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)