摘要
建立同时测定西洋参中7种人参皂苷含量的快速检测方法.方法:采用超高效液相色谱法,ACQUITYUPLCBEHC18色谱柱(2.1×50mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速:0.5ml/min,柱温35℃,进样体积2μL,检测波长203nm.结果:人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd存在良好线性关系,线性范围分别为0.0067~0.334mg/ml,0.0151~0.756mg/ml,0.0130~0.649mg/ml,0.0043~0.217mg/ml,0.0044~0.221mg/ml,0.0085~0.424mg/ml,0.0086~0.43mg/ml.结论:该方法准确、重现性好,可用于西洋参中人参皂苷含量测定.
出版日期
2013年04月14日(中国期刊网平台首次上网日期,不代表论文的发表时间)