STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES-中国期刊网

摘要 Thepropertiesofimmobilizedglucoseoxidase(GOD)bythecomplexesofdiethylaminoethylcellulose(DEAEC)withdifferentpolymers,suchaspolymethylacrylicacid(PMAA),polyacrylicacid(PAA),polystyrenesulfonicacid(PSSA),polyvinylaleohol(PVA),polyethyleneoxide(PEO)andstyrene-maleicacidcopolymer(PSMA)wereinvestigated.TheactivityofimmobilizedGODwasobviouslyinfluencedbythecomponentoftheDEAECcomplexes.TherelativeactivityoftheimmobilizedGODreachedtomaximumandover90%ofthenativeGOD.whentheDEAEC-PMAADEAEC-PAAcomplexeswereusedasacarrierwiththemolarratioofDEAECandpolyacidofaboutone.Michaelisconstants(Km)oftheimmobilizedenzymesofDEAEC-GOD-PMAAandDEAEC-GOD-PAAweredeterminedtobe1.25and1.00,respectively.Moreover,theimmobilizedGODhasagoodstoragestabilityandcycliclife.

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STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES

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STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES

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