简介:Objective:Toinvestigatethepost-transcriptionalregulationofp21WAF1/CIP1byp53.Methods:TheMDA-MB-468cellshaveendogenousmutantp53andtheMCF7cellslineshavewtp53.Recombinantp53expressionandp21WAF1/CIP1inductionweredetectedbyWesternblotanalysis.Northernblotanalysiswascarriedouttoexaminewhetherchangesinp21WAF1/CIP1proteinlevelsinMCF7cellstreatedwithAdCMVp53arereflectedatthemRNAlevel.FlowcytometricanalysisofMCF7cellsfollowingoverexpressionofrecombination.Results:Theratioofp53:p21WAF1/CIP1wasbelow1attheearlystagesofAdCMVp53infection,butincreasedto1.6byday3andto9.7byday5post-infection.Asexpected,p21WAF1/CIP1expressionwasnotdetectableinMDA-MB-468cellsdespitethepresenceofhighlevelsofmutantp53protein.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.ByNorthernblotanalyzingthep21WAF1/CIP1:GAPDHratiosatdifferenttimepointsagainsttheratioattimepoint0,amaximum3-foldinductionofp21WAF1/CIP1mRNAexpressionrelativetountreatedcontrolwasobservedonday1post-infection.TheflowcytometricanalysisindicatedthatMCF7cellsinfectedwithAdCMVp53undergoG1arrestatbothtimepointsstudied,withG1/Sratiosrangingfrom5.54atday1to5.65atday7.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.Conclusion:Thisstudydemonstratedthatp53couldregulatep21WAF1/CIP1geneexpressionatboththetranscriptionalandpost-transcriptionallevelsinMCF7cells.Thelattermechanismmaybeinvolvedinorberesponsiblefor,theinductionofcellcyclearrestbytranscription-defectivemutantsofp53.
简介:妊娠合并脑瘤后,由于早孕反应和晚期妊娠中毒,出现头痛、恶心、呕吐等症状,易与脑瘤所致之颅内压增高的症状相混淆,延误诊治。一28岁女性患者,因停经8月,头痛、恶心、呕吐,右上、下肢无力半月,于2003年6月7日入我院。检查:神志清楚,右侧上、下肢肌力Ⅲ级,肌张力增高,右侧Babinski征阳性。腹围91cm,宫高31cm,头先露,浮,胎心144次/分。MRI检查:左额叶4×3.5×5cm分叶状占位病变。T1等、低、混杂信号,T2高、等、混杂信号。周围脑白质水肿,灰质受压,左侧脑室变窄,中线结构右移。诊断:右额叶胶质瘤,妊娠8月。2003年6月17日手术,先在腰麻下行剖腹产,娩出2250克重男婴,成活良好。随即
简介:GFAPisaspecificantigenofglialelement,butAlpha-1-antichymotrypsinhasnotbeenreportedintheliterature.Alpha-1-antichymotrypsinwasguidedbyGFAPusingPAPmethodtotheastrocytesof137gliomas.120(87%)gliomaswerepositiveforAlpha-1-antichymotrypsin.Ofthese120gliomas,86(72%)gavediffusedistribution,17(14%)gavefocaldistribution,and17(14%)gavescattereddistributions.Alpha-1-antichymotrypsiningliomatissuemaybeanimportanttumormarkerfordiagnosis.